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Bone Repair With Differentiated Osteoblasts From Adipose-Derived Stem Cells in Hydroxyapatite/Tricalcium Phosphate in Vivo Publisher



Hashemibeni B1 ; Dehghani L2 ; Sadeghi F1 ; Esfandiari E1 ; Gorbani M3, 4 ; Akhavan A5 ; Tahani ST2, 6 ; Bahramian H1 ; Goharian V7, 8
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Authors Affiliations
  1. 1. Department of Anatomical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Medical Sciences, Islamic Azad University, Najafabad Branch, Isfahan, Iran
  3. 3. Department of Tissue Engineering and Cell Therapy, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Applied Biotechnology Center, Pajooheshgah Baqiatallah University and Medical Sciences, Tehran, Iran
  5. 5. Torabinegad Dental Research Center, Dental School, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Isfahan Neurosciences Research Center, Al Zahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
  7. 7. Department of Surgery, Amine Hospital, Isfahan, Iran
  8. 8. Institute of Novin Tahlilgarane Nesfe-Jahan, Isfahan, Iran

Source: International Journal of Preventive Medicine Published:2016


Abstract

Background: Recently, tissue engineering has developed approaches for repair and restoration of damaged skeletal system based on different scaffolds and cells. This study evaluated the ability of differentiated osteoblasts from adipose-derived stem cells (ADSCs) seeded into hydroxyapatite/ tricalcium phosphate (HA-TCP) to repair bone. Methods: In this study, ADSCs of 6 canines were seeded in HA-TCP and differentiated into osteoblasts in osteogenic medium in vitro and bone markers evaluated by reverse transcription polymerase chain reaction (RT-PCR). Scanning electron microscopy (SEM) was applied for detection of cells in the pores of scaffold. HA-TCP with differentiated cells as the test group and without cells as the cell-free group were implanted in separate defected sites of canine’s tibia. After 8 weeks, specimens were evaluated by histological, immunohistochemical methods, and densitometry test. The data were analyzed using the SPSS 18 version software. Results: The expression of Type I collagen and osteocalcin genes in differentiated cells were indicated by RT-PCR. SEM results revealed the adhesion of cells in scaffold pores. Formation of trabecular bone confirmed by histological sections that revealed the thickness of bone trabecular was more in the test group. Production of osteopontin in extracellular matrix was indicated in both groups. Densitometry method indicated that strength in the test group was similar to cell-free group and natural bone (P > 0.05). Conclusions: This research suggests that ADSCs-derived osteoblasts in HA-TCP could be used for bone tissue engineering and repairing. © 2016 International Journal of Preventive Medicine.
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