Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Comparison of Phenotypic Characterization Between Differentiated Osteoblasts From Stem Cells and Calvaria Osteoblasts in Vitro



Hashemibeni B1 ; Jafary F2 ; Esmaeil N3 ; Goharian V4, 5 ; Feizi G6 ; Heidari F6 ; Moosavinejad SZ2 ; Zarkesh SH3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomical Sciences and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Biology, Sciences School, Alzahra University, Tehran, Iran
  3. 3. Department of Immunology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Surgery, Amine hospital, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. Institute of Novin Tahlilgarane Nesfe-Jahan, Isfahan, Iran
  6. 6. Torabinegad Research Center, Department of Endodontics, Dental School, Isfahan University of Medical Sciences, Isfahan, Iran

Source: International Journal of Preventive Medicine Published:2013

Abstract

Background: Characteristics of differentiated osteoblasts from adipose derived stem cells (ADSCs) in compared with isolated osteoblasts from normal bone such as calvaria are unknown. The aim of this study was determination and comparison of phenotypic characterization between differentiated osteoblasts from stem cells and calvaria osteoblasts in vitro. Methods: In this study, mesenchymal stem cells were isolated from adipose tissue of human by enzymatic digestion and were differentiated into osteoblasts using osteogenic medium. Characteristics of these cells at first, second, third and fourth weeks were comprised with calvaria osteoblasts that were isolated from human calvaria by explanation culture method. To screen the characteristics of both calvaria and the differentiated osteoblasts, we used western blot to identify protein levels, von Kossa staining for mineral matrix detection and alkaline phosphatase (ALP) assay kit (Sigma) for ALP activity measurement. Difference between calvaria and differentiated osteoblast cells were analyzed by one-way ANOVA and P < 0.05 was considered as statistically significant. Results: Alkaline phosphatase activity, collagen and mineral material production in differentiated osteoblasts at third week were more significantly than calvaria cells (P < 0.05). Our results indicated that there was no significant different in osteocalcin (OC) production between differentiated osteoblast at first, second and third weeks and calvaria cells but declined at fourth week (P < 0.05). Conclusions: Our survey showed that cellular traits of differentiated osteoblasts presented better than calvaria osteoblasts in vitro conditions. Therefore, we suggest that ADSCs could be used in next studies for bone tissue engineering.
Related Docs
View other Related Docs
1. An Animal Model Study for Bone Repair With Encapsulated Differentiated Osteoblasts From Adipose-Derived Stem Cells in Alginate, Iranian Journal of Basic Medical Sciences (2014)
2. Comparative Study of Microtubule-Associated Protein-2 and Glial Fibrillary Acidic Proteins During Neural Induction of Human Bone Marrow Mesenchymal Stem Cells and Adipose-Derived Stem Cells, International Journal of Preventive Medicine (2014)
3. Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells With Tgf-Β3 in Pellet Culture System, Iranian Journal of Basic Medical Sciences (2008)
4. Histopathological Comparison Between Bone Marrow- and Periodontium-Derived Stem Cells for Bone Regeneration in Rabbit Calvaria, International Journal of Organ Transplantation Medicine (2016)
5. Effects of Human Placental Serum on Proliferation and Morphology of Human Adipose Tissue-Derived Stem Cells, Bone Marrow Transplantation (2011)
6. Bone Repair With Differentiated Osteoblasts From Adipose-Derived Stem Cells in Hydroxyapatite/Tricalcium Phosphate in Vivo, International Journal of Preventive Medicine (2016)
7. The Expression of Osteogenic Markers in Differentiated Osteoblasts From Adipose-Derived Stem Cells in Pellet Culture and Monolayer Systems, Journal of Isfahan Medical School (2012)
8. Stability of Neural Differentiation in Human Adipose Derived Stem Cells by Two Induction Protocols, Tissue and Cell (2012)
Experts (# of related papers)
View all Related Experts
Batool Hashemibeni (17)
Ebrahim Esfandiari (13)
Other Related Docs
9. Adipose-Derived Stem Cells Combined With Beta-Tricalcium Phosphate: A Novel Possible Strategy for Periodontal Defects Regeneration, Medical Hypotheses (2014)
10. Effects of Nano-Biphasic Calcium Phosphate Composite on Bioactivity and Osteoblast Cell Behavior in Tissue Engineering Applications, Journal of Medical Signals and Sensors (2016)
11. The Role of Calcium in Differentiation of Human Adipose-Derived Stem Cells to Adipocytes, Molecular Biotechnology (2018)
12. Isolation of Mesenchymal Stem Cells From Dental Pulp of Exfoliated Human Deciduous Teeth, Yakhteh (2008)
13. An Overview of Neural Differentiation Potential of Human Adipose Derived Stem Cells, Stem Cell Reviews and Reports (2016)
14. Comparison of Aggrecan Gene Expression in Chondrogenesis of Adipose-Derived Stem Cells in Pellet and Micromass Culture Systems, Tehran University Medical Journal (2018)
15. Poly (Glycerol Sebacate) and Polyhydroxybutyrate Electrospun Nanocomposite Facilitates Osteogenic Differentiation of Mesenchymal Stem Cells, Journal of Drug Delivery Science and Technology (2021)
16. Modification of Porous Calcium Phosphate Surfaces With Different Geometries of Bioactive Glass Nanoparticles, Materials Science and Engineering C (2012)
17. Preparation, Chemistry and Physical Properties of Bone-Derived Hydroxyapatite Particles Having a Negative Zeta Potential, Materials Chemistry and Physics (2012)
18. Fabrication of Elastin Additive on Polymethyl Methacrylate and Hydroxyapatite-Based Bioactive Bone Cement, Materials Chemistry and Physics (2022)
19. The Characteristics of Human Osteoblast Cells in Two Methods of Monolayer Culture in Hydroxyapatite-Tricalcium Phosphate Scafolds, Journal of Isfahan Medical School (2011)
20. Therapeutic Application of Multipotent Stem Cells, Journal of Cellular Physiology (2018)
21. Calcitonin-Loaded Octamaleimic Acid–Silsesquioxane Nanoparticles in Hydrogel Scaffold Support Osteoinductivity in Bone Regeneration, Pharmaceutical Development and Technology (2021)
22. Current Advances in Stem Cell Therapy in the Treatment of Multiple Sclerosis, Reviews in the Neurosciences (2023)
23. The Effect of Bmp-6 Growth Factor on Differentiation of Adipose-Derived Stem Cells Into Chondrocyte in Pellet Culture System, Journal of Isfahan Medical School (2009)
24. Relation Between Biochemical Parameters and Bone Density in Postmenopausal Women With Osteoporosis, Advanced Biomedical Research (2023)
25. Comparison Between Chondrogenic Markers of Differentiated Chondrocytes From Adipose Derived Stem Cells and Articular Chondrocytes in Vitro, Iranian Journal of Basic Medical Sciences (2013)
26. Overview of Retinal Differentiation Potential of Mesenchymal Stem Cells: A Promising Approach for Retinal Cell Therapy, Annals of Anatomy (2017)
27. Induction of Mineralized Nodule Formation in Rat Bone Marrow Stromal Cell Cultures by Silk Fibroin, Iranian Biomedical Journal (2006)
28. Transcriptomic Evaluation of Cbfa1 Gene During Osteogenic Differentiation of Adipose Derived Mesenchymal Stem Cells, Journal of Isfahan Medical School (2013)
29. Comparison of Cd44 and Cd14 Surface Markers in Adipose-Derived Stem Cells and Differentiated Chondrocytes During Chondrogenesis, Journal of Isfahan Medical School (2014)
30. The Relationship Between Maternal Vitamin D Levels and Osteopenia Development in Preterm Infants: A Cross-Sectional Study, International Journal of Reproductive BioMedicine (2024)
31. Preparation and Characterization of Silk-Chitosan Composite As a Three- Dimensional Tool for Culturing Osteoblast-Like Cells, Journal of Isfahan Medical School (2015)
32. Comparison of Human Osteoblast Proliferation in Alginate and Hydroxyapatite-Tricalcium Phosphate Scafolds, Journal of Isfahan Medical School (2010)
33. Promoting Effect of Nano Hydroxyapatite and Vitamin D3 on the Osteogenic Differentiation of Human Adipose-Derived Stem Cells in Polycaprolactone/Gelatin Scaffold for Bone Tissue Engineering, Materials Science and Engineering C (2019)
34. Effect of Transforming Growth Factor-Β3 and Bone Morphogenetic Protein-6 Growth Factors on Chondrogenic Differentiation of Adipose-Derived Stem Cells in Alginate Scaffold, Journal of Isfahan Medical School (2010)
35. Effects of Fgf-2 on Human Adipose Tissue Derived Adult Stem Cells Morphology and Chondrogenesis Enhancement in Transwell Culture, Biochemical and Biophysical Research Communications (2012)
36. Isolation and Differentiation of Adipose-Derived Stem Cells Into Odontoblast-Like Cells: A Preliminary in Vitro Study, Cell Journal (2021)
37. Application of Mesenchymal Stem Cells in Melanoma: A Potential Therapeutic Strategy for Delivery of Targeted Agents, Current Medicinal Chemistry (2016)
38. In Vivo Assessment of Bone Repair by an Injectable Nanocomposite Scaffold for Local Co-Delivery of Autologous Platelet-Rich Plasma and Calcitonin in a Rat Model, Drug Development and Industrial Pharmacy (2022)
39. Effects of Estrogen on the Expression of Neural Markers in Differentiated Adipose-Derived Stem Cells, Journal of Isfahan Medical School (2015)
40. Comparison of Autogenous Bone Graft and Tissue-Engineered Bone Graft in Alveolar Cleft Defects in Canine Animal Models Using Digital Radiography, Indian Journal of Dental Research (2020)
41. Overview of Bone Graft Materials, Osteomyelitis and Discitis of the Spine: A Guide for the Management of Infectious Etiology of the Vertebral Column and Neurological Components (2024)
42. Combined Effect of Retinoic Acid and Calcium on the in Vitro Differentiation of Human Adipose-Derived Stem Cells to Adipocytes, Archives of Physiology and Biochemistry (2018)
43. Investigating the Effect of Human Adipose Stem Cell-Derived Exosomes on Survival and Neural Differentiation of Pc12 Cells, Journal of Isfahan Medical School (2019)
44. Potential Probiotic Lactobacillus Delbrueckii Subsp. Lactis Kums-Y33 Suppresses Adipogenesis and Promotes Osteogenesis in Human Adipose-Derived Mesenchymal Stem Cell, Scientific Reports (2024)