Echhinococcus Granulosus Lnfection in Mice Following Lmmunization With Protoscoleces Antigens



Haghpanah B¹ ; Ghayur Z¹ ; Ahmadi N¹ ; Hejazi H¹ Show Affiliations
Source: Journal of Isfahan Medical School Published:2009

Abstract

Background: Hydatidosis, caused by the metacestode stage of Echinococcus granulosus, is a major zoonotic disease of worldwide distribution. Any success in recognition of immunological reaction of intermediate hosts and in production of vaccine can lead to a decrease in human infections and also in economic loss of animals. Methods: Immunization was achieved by inject on of surface proteins of Echinococcus granulosus protoscoleces (PSC) in 30 male mice (C57 BL/C6). Surface proteins of PSC were prepared by using of solution nonionic detergent Mega-10. Then 100 µg of this protein was emulsified in Freunds Incomplete Adjuvant (FIA) and injected intraperitoneally to immunized mice and 15 normal mice as a control group. For immunization, mice were boosted similarly after 27 days and they were challenged by intraperitoneally injection of 2000 bovine viable protoscoleces after two weeks. Resistant rate (size, weight, number of cysts) were determined after challenge. Findings: Autopsies of mice were carried out 240 days post challenge. In immunized mice, cyst either were not observed, or in compare with control group smaller cysts were observed. The mean weight of cysts was 0.39 ± 0.36 and 1.7 ± 1.8 grams in immunized and control groups respectively (P < 0.015). The average size of cysts in these two groups were 10.7 ± 15.6 and 7.4 ± 4.2 mm (P < 0.006), and the mean number of cysts were 10.7 ± 15.6 and 52.6 ± 44.2 (P < 0.002) respectively. Conclusion: In this study the observed protection with immunization was 76.6-79.7 percent. Results of this study showed that surface protein of protoscoleces emulsified in Freunds Incomplete Adjuvant probably can be used for immunization of intermediate host. © 2009, Isfahan University of Medical Sciences(IUMS). All rights reserved.