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Effect of Hydatid Cyst Antigens Polyspecific Antisera on Breast Cancer Cells (4T1) Growth in Cell Culture Medium Publisher



Darani HY1, 2 ; Sharafi SM3
Authors
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Authors Affiliations
  1. 1. Cancer Prevention Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Environment Research Center, Research Institute For Primordial Prevention of Noncommunicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran

Source: International Journal of Environmental Health Engineering Published:2022


Abstract

Aim: Hydatid cyst is the Echinococcus granulosus larvae stage and is responsible for echinococcosis. Anticancer effects of hydatid cyst have been shown in human population, experimental animals, and in vitro works. However, the mechanisms of this anticancer activity are not clarified. Hence, in this work, the effect of antisera raised against hydatid cyst antigens on the growth of breast cancer cells has been investigated. Materials and Methods: In this experimental study, the various hydatid cyst antigens were prepared. To raise antisera, each of the hydatid cyst antigens (hydatid cyst fluid, germinal and laminated, protoscolex, and excretory-secretory [ES]) and toxoplasma trophozoite were injections to rabbits. The production of specific antibodies in rabbits determinate by enzyme-linked immunoassay, and then, the rabbit’s blood was taken, and their sera isolated under the hood in sterile conditions. Before use, all antisera were inactivated at 56°C for half an hour and also placed under ultraviolet light for 20 min to disinfect. Breast cancer cells in the culture medium were purchased from the Pasteur Institute of Iran and growth in CO2 incubator in the Roswell Park Memorial Institute (RPMI) medium. After appropriate, the cells were counted and divided equally in eight-cell culture flasks and treated with different antisera of hydatid cyst. After 32-h incubation, the number of live cells was counted by trypan blue methods and compared with control groups. Results: Based on the results of this research, the difference between the number of live cells after treatment with antisera against hydatid cyst fluid, toxoplasma trophozoite, and ES antigen was significantly different from number of cells in flask treated with normal rabbit serum. Conclusion: In conclusion, due to the presence of common antigens between parasites and cancer, probably antibodies produced against hydatid cyst antigens may affect the growth of cells in the culture media. © 2022 International Journal of Environmental Health Engineering.
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