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Immunohistochemical Observation of Local Inflammatory Cell Infiltration in the Host-Tissue Reaction Site of Human Hydatid Cysts Publisher Pubmed



Jafari R1 ; Sanei B2 ; Baradaran A3 ; Kolahdouzan M2 ; Bagherpour B4 ; Yousofi Darani H1
Authors
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Authors Affiliations
  1. 1. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Surgery, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Acquired Immunodeficiency Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Helminthology Published:2019


Abstract

The aim of this study was to evaluate the pattern of local immune cell infiltration in human cystic echinococcosis (CE) by identifying the subtypes of immune cells using immunohistochemistry (IHC). Fifty surgically removed hydatid cyst samples and surrounding tissues were collected from patients referred to Al-Zahra Hospital, Isfahan, Iran. IHC was performed on the surrounding host tissue of hydatid cysts using anti-human CD3, CD19, CD8, CD4, CD68, CD56, Ki-67 and Foxp3 (forkhead box P3) antibodies. The results were then compared to hepatocellular carcinoma and chronic hepatitis. In the host-tissue reaction site of liver hydatid cysts, a distinct pattern of local immune cell response, which outwardly consisted of a pack of the fibrous elements, a layer of palisading macrophages, an eosinophil-containing layer and a layer of accumulated lymphocytes, was observed. However, in some cases there were no positive cells for CD56+ natural killer cells and Foxp3+ regulatory T cells. The CD3+ T cells were the predominant inflammatory cells in all groups, followed by CD19+ B cells. It can be concluded that different immune cells are involved in the local response to human hydatid cysts. Copyright © Cambridge University Press 2018.
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