Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Preparation and in Vivo Evaluation of a Novel Gel-Based Wound Dressing Using Arginine–Alginate Surface-Modified Chitosan Nanofibers Publisher Pubmed



Najar MH1 ; Minaiyan M2 ; Taheri A1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Novel Drug Delivery Systems Research Center, Department of Pharmaceutics, Faculty of Pharmacy, Isfahan University of Medical sciences, Isfahan, Iran
  2. 2. Department of Pharmacology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Biomaterials Applications Published:2018


Abstract

The development of an effective wound dressing with the ability to induce skin wound healing is a great challenge in medicine. Nanofibers are highly attractive for wound dressing preparation due to their properties such as hemostasis induction, good absorption of wound exudates, and facilitation of cell growth. Chitosan nanofibers have attracted great attention for application in wound dressings due to their accelerating effects on wound healing. In this study, arginine surface-modified chitosan nanofibers were successfully prepared by attachment of arginine molecules on the surface of chitosan nanofibers using sodium alginate through electrostatic interaction. The effect of pH on the amount of attached arginine was evaluated at three different pH values; 5, 6, and 7. Fourier-transform infrared spectroscopy and zeta potential of chitosan nanofibers before and after surface modification suggested the occurrence of the attachment of arginine to chitosan nanofibers. Scanning electron microscope images showed the nanofibrous structure of arginine surface-modified chitosan nanofibers with an average diameter ranging from 100 nm to 150 nm. The release of arginine from arginine surface-modified chitosan nanofibers gel showed a sustained release manner. The suitable viscosity and spreadability of arginine surface-modified chitosan nanofibers gel verified its easy application at the wound site. Arginine surface-modified chitosan nanofibers gel significantly improved the wound healing process including wound closure when tested in vivo using rat model. Additionally, histological examination and immunohistochemical studies showed the significant enhancement of the re-epithelialization, collagen deposition, and angiogenesis in the skin of the animal group treated with arginine surface-modified chitosan nanofibers gel compared with the other control groups. These results suggested that arginine surface-modified chitosan nanofibers gel could be introduced as an effective wound dressing. © The Author(s) 2017.
Related Docs
View other Related Docs
1. A Novel Lignin-Based Nanofibrous Dressing Containing Arginine for Wound-Healing Applications, Drug Delivery and Translational Research (2018)
2. Arginine Functionalized Bacterial Cellulose Nanofibers Containing Gel As an Effective Wound Dressing: In Vitro and in Vivo Evaluation, Current Drug Delivery (2018)
3. Nanocrystalline Cellulose-Hyaluronic Acid Composite Enriched With Gm-Csf Loaded Chitosan Nanoparticles for Enhanced Wound Healing, Biomedical Materials (Bristol) (2019)
4. A Novel Bilayer Drug-Loaded Wound Dressing of Pvdf and Phb/Chitosan Nanofibers Applicable for Post-Surgical Ulcers, International Journal of Polymeric Materials and Polymeric Biomaterials (2019)
5. Preparation and Characterization of Trimethyl Chitosan /Hyaluronic Acid Composite Encapsulated With Ll37 to Promote Wound Healing in an Animal Model, International Journal of Peptide Research and Therapeutics (2023)
6. Preparation of a Biomimetic Bi-Layer Chitosan Wound Dressing Composed of A-Prf/Sponge Layer and L-Arginine/Nanofiber, Carbohydrate Polymers (2022)
7. Development of Novel Topical Formulation From Fullerene With Antibacterial Activity Against Propionibacterium Acnes, Fullerenes Nanotubes and Carbon Nanostructures (2020)
8. Alginate-Based Nanocomposite Incorporating Chitosan Nanoparticles: A Dual-Drug Delivery System for Infection Control and Wound Regeneration, Journal of Drug Delivery Science and Technology (2025)
Experts (# of related papers)
View all Related Experts
Jaleh Varshosaz (31)
Saeed Karbasi (21)
Other Related Docs
9. Antibacterial Self-Healing Bilayer Dressing for Epidermal Sensors and Accelerate Wound Repair, Carbohydrate Polymers (2023)
10. Alginate Hydrogel Enriched With Ambystoma Mexicanum Epidermal Lipoxygenase-Loaded Pectin Nanoparticles for Enhanced Wound Healing, Journal of Biomaterials Applications (2020)
11. Electrospun Core-Shell Polycaprolactone/ Chitosan Nanofibrous Composite With Enhanced Curcumin Loading Capacity for Wound Healing Applications, Materials Today Chemistry (2024)
12. Physical and Biological Properties of Blend-Electrospun Polycaprolactone/Chitosan-Based Wound Dressings Loaded With N-Decyl-N, N-Dimethyl-1-Decanaminium Chloride: An in Vitro and in Vivo Study, Journal of Biomedical Materials Research - Part B Applied Biomaterials (2020)
13. Fabrication and Characterisation of Chitosan/Polyvinyl Alcohol-Based Transparent Hydrogel Films Loaded With Silver Nanoparticles and Sildenafil Citrate for Wound Dressing Applications, Materials Technology (2022)
14. Preparation and Characterization of Metformin Surface Modified Cellulose Nanofiber Gel and Evaluation of Its Anti-Metastatic Potentials, Carbohydrate Polymers (2017)
15. Acceleration of Wound Healing in Rats by Modified Lignocellulose Based Sponge Containing Pentoxifylline Loaded Lecithin/Chitosan Nanoparticles, Gels (2022)
16. Electrosprayed Cefazolin-Loaded Niosomes Onto Electrospun Chitosan Nanofibrous Membrane for Wound Healing Applications, Journal of Biomedical Materials Research - Part B Applied Biomaterials (2022)
17. Core-Shell Nanofibers Containing L-Arginine Stimulates Angiogenesis and Full Thickness Dermal Wound Repair, International Journal of Pharmaceutics (2024)
18. Preparation of an Injectable Doxorubicin Surface Modified Cellulose Nanofiber Gel and Evaluation of Its Anti-Tumor and Anti-Metastasis Activity in Melanoma, Biotechnology Progress (2018)
19. Antimicrobial Synthetic and Natural Polymeric Nanofibers As Wound Dressing: A Review, Advanced Engineering Materials (2022)
20. Fabrication and Characterization of Glycerol/Chitosan/Polyvinyl Alcohol-Based Transparent Hydrogel Films Loaded With Silver Nanoparticles for Antibacterial Wound Dressing Applications, Advanced Biomedical Research (2021)
21. An in Vitro and in Vivo Study of Pcl/Chitosan Electrospun Mat on Polyurethane/Propolis Foam As a Bilayer Wound Dressing, Biomaterials Advances (2022)
22. Novel Electrospun Polyvinyl Alcohol/Chitosan/Polycaprolactone-Diltiazem Hydrochloride Nanocomposite Membranes for Wound Dressing Applications, Emergent Materials (2024)
23. Fabrication and Evaluation of 3D Bio-Scaffold Wound Dressings for Monitoring of Chronic Ph Wounds Using Fuzzy Logic Analysis, Materials Science and Engineering: B (2023)
24. An in Vitro and in Vivo Study of Electrospun Polyvinyl Alcohol/Chitosan/Sildenafil Citrate Mat on 3D-Printed Polycaprolactone Membrane As a Double Layer Wound Dressing, International Journal of Biological Macromolecules (2024)
25. Effects of Multi-Wall Carbon Nanotubes on Structural and Mechanical Properties of Poly(3-Hydroxybutyrate)/Chitosan Electrospun Scaffolds for Cartilage Tissue Engineering, Bulletin of Materials Science (2017)
26. Transferrin-Targeted Poly(Butylene Adipate)/Terephthalate Nanoparticles for Targeted Delivery of 5-Fluorouracil in Ht29 Colorectal Cancer Cell Line, Journal of Bioactive and Compatible Polymers (2017)
27. An Injectable Carboxymethyl Chitosan-Methylcellulose-Pluronic Hydrogel for the Encapsulation of Meloxicam Loaded Nanoparticles, International Journal of Biological Macromolecules (2020)
28. Fabrication and Characterization of Chitosan-Gelatin/Single-Walled Carbon Nanotubes Electrospun Composite Scaffolds for Cartilage Tissue Engineering Applications, Polymers for Advanced Technologies (2022)
29. Regeneration of Rat Sciatic Nerve Using Plga Conduit Containing Rat Adscs With Controlled Release of Bdnf and Gold Nanoparticles, Journal of Molecular Neuroscience (2021)
30. Evaluation of the Effects of Decellularized Wharton Jelly Nanoparticles on Polyhydroxy Butyrate-Chitosan Electrospun Scaffolds for Cartilage Tissue Engineering Applications, Journal of Polymers and the Environment (2025)
31. Effect of Polymer Molecular Weight on Morphology and Particle Size of Chitosan Microspheres Prepared Via Spray Drying Method, IFMBE Proceedings (2009)
32. Effects of Nanozeolite/Starch Thermoplastic Hydrogels on Wound Healing, Journal of Research in Medical Sciences (2017)
33. Evaluation of the Effects of Chitosan Nanoparticles on Polyhydroxy Butyrate Electrospun Scaffolds for Cartilage Tissue Engineering Applications, International Journal of Biological Macromolecules (2023)
34. Ciprofloxacin Loaded Alginate/Chitosan and Solid Lipid Nanoparticles, Preparation, and Characterization, Journal of Dispersion Science and Technology (2012)
35. Polycaprolactone-Chitosan/Multi-Walled Carbon Nanotube: A Highly Strengthened Electrospun Nanocomposite Scaffold for Cartilage Tissue Engineering, International Journal of Biological Macromolecules (2022)
36. Keratin- and Vegf-Incorporated Honey-Based Sponge-Nanofiber Dressing: An Ideal Construct for Wound Healing, ACS Applied Materials and Interfaces (2023)
37. Platelet Rich Fibrin Containing Nanofibrous Dressing for Wound Healing Application: Fabrication, Characterization and Biological Evaluations, Materials Science and Engineering C (2021)
38. A Novel Wound Dressing Nanofiber With Anti-Inflammatory and Anti-Bacterial Drugs Release for Skin Wound Healing, Journal of Isfahan Medical School (2019)
39. Preparation and in Vitro Evaluation of Vancomycin Loaded Montmorillonite-Sodium Alginate Topical Gel for Wound Infection, Brazilian Journal of Pharmaceutical Sciences (2022)
40. Design and Optimization of Alginate-Chitosan-Pluronic Nanoparticles As a Novel Meloxicam Drug Delivery System, Journal of Applied Polymer Science (2015)
41. A High-Efficient Antibacterial and Biocompatible Polyurethane Film With Ag@Rgo Nanostructures Prepared by Microwave-Assisted Method: Physicochemical and Dermal Wound Healing Evaluation, Heliyon (2023)
42. Immobilization of Alkaline Phosphatase Using Chitosan Nanoparticles for Enhancing Its Stability, Russian Journal of Applied Chemistry (2014)
43. Clinical Assessment of Novel Nanofibrous Dressings for Cutaneous Leishmaniasis: A Trial-Based Study, Polymers for Advanced Technologies (2024)
44. Multilayer Pva/Gelatin Nanofibrous Scaffolds Incorporated With Tanacetum Polycephalum Essential Oil and Amoxicillin for Skin Tissue Engineering Application, International Journal of Biological Macromolecules (2024)
45. Fabrication and Characterization of Injectable Thermosensitive Hydrogel Containing Dipyridamole Loaded Polycaprolactone Nanoparticles for Bone Tissue Engineering, Journal of Drug Delivery Science and Technology (2021)
46. Recent Advances in Electrospun Protein Fibers/Nanofibers for the Food and Biomedical Applications, Advances in Colloid and Interface Science (2023)
47. Assessing the Physical and Mechanical Properties of Poly 3-Hydroxybutyrate-Chitosan-Multi-Walled Carbon Nanotube/Silk Nano-Micro Composite Scaffold for Long-Term Healing Tissue Engineering Applications, Micro and Nano Letters (2018)
48. Mupirocin Loaded Core-Shell Pluronic-Pectin-Keratin Nanofibers Improve Human Keratinocytes Behavior, Angiogenic Activity and Wound Healing, International Journal of Biological Macromolecules (2023)
49. Rgd Peptide Grafted Polybutylene Adipate-Co-Terephthalate/Gelatin Electrospun Nanofibers Loaded With a Matrix Metalloproteinase Inhibitor Drug for Alleviating of Wounds: An In Vitro/In Vivo Study, Drug Development and Industrial Pharmacy (2020)
50. Preparation, Characterization and Antibacterial Activities of Chitosan, N-Trimethyl Chitosan (Tmc) and N-Diethylmethyl Chitosan (Demc) Nanoparticles Loaded With Insulin Using Both the Ionotropic Gelation and Polyelectrolyte Complexation Methods, International Journal of Pharmaceutics (2008)