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Evaluation of Hydrostatic Pressure on Metabolism of the Articular Chondrocytes Seeded on Biodegradable Polyurethane As Tissue Engineering Scaffold Publisher



Karbasi S1
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Authors Affiliations
  1. 1. Biomedical Engineering Department, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran

Source: IFMBE Proceedings Published:2007


Abstract

One of the most effective parameters in articular cartilage tissue engineering is cell stimulation and growth. Using a reaction system which incorporates many of the same conditions as an articulating joint such as intermittent loading, long term culture and a suitable 3-D growth environment could stimulate chondrocytes metabolism. It has demonstrated that hydrostatic pressure, as a physicochemical parameter, also could increase chondrocytes metabolism in tissue constructs. In this research, the effect of hydrostatic pressure was investigated on lactate production and GAG (glycosaminoglycane) content produced by chondrocytes. The isolated chondrocytes, from animal joint, were seeded on a biodegradable polyesterurethane scaffold (BPUS) namely Degrapol® and 4 MPa was applied to the samples for 4 h per day as a cyclic (1HZ, Sinusoidal) load. The results showed that in constant physicochemical conditions, the hydrostatic pressure could increase the amount of lactate, rate of lactate production and GAG as a significant cartilage metabolism on BPUS. In comparison to other biodegradable polymers, this research was showed that BPUS has great potential for articular cartilage tissue engineering, in vitro. © International Federation for Medical and Biological Engineering 2007.
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