A Comparative Study of Articular Chondrocytes Metabolism on a Biodegradable Polyesterurethane Scaffold and Alginate in Different Oxygen Tension and Ph Publisher



Karbasi S¹ Show Affiliations
Source: IFMBE Proceedings Published:2009

Abstract

There are some different methods in the literatures that used for healing and repairing of cartilage. One of the methods for increasing of regeneration and metabolism in cartilage, is stimulating physicochemical parameters on cellpolymer systems, as cartilage based cells. In this research, two physicochemical parameters, oxygen tension and pH, was changed to measure the lactate production after 1, 2 and 3 days culture and GAG(glycosaminoglycan) production after 3, 7 and 14 days culture of chondrocytes on DegraPol®, as a biodegradable polyurethane scaffold (BPUS), and alginate scaffolds. The results finally were compared on both scaffolds. The results showed that physicochemical parameters like oxygen tension and pH could change cell metabolism. In fact, the physicochemical parameters could affect lactate production and GAG content of chondrocyte cells and it does not depend on the type of scaffold. The best condition of the articular chondrocytes metabolism was for 5% O2 and pH=7.4(p<0.001). The comparison between BPUS and alginate scaffold is showing that the results are better for alginate beads (p<0.001). In fact, hydrophilicity of alginate causes better cell distribution and nutrition than BPUS; because the cells are able to transfer the ions and the products through the medium easily.