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Evaluation of Sars-Cov-2 Genome Detection by Real-Time Pcr Assay Using Pooled Specimens Publisher



Tavakoli F1, 2 ; Yavarian J1 ; Shafiei Jandaghi NZ1 ; Sadeghi K1 ; Ghavami N1 ; Salimi V1 ; Mokhtariazad T1
Authors
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Authors Affiliations
  1. 1. Virology Department, School Of Public Health, Tehran University Of Medical Sciences, Tehran, Iran
  2. 2. Department Of Bacteriology And Virology, Faculty Of Medicine, Isfahan University Of Medical Science, Isfahan, Iran

Source: Future Virology Published:2022


Abstract

Objective: To evaluate SARS-CoV-2 genome detection using pooled samples by RT-qPCR assay, compared to individual samples. Method: At first all samples were tested individually using two commercial methods targeting ORF1ab, NP and E genes. Then, four experimental groups of samples were pooled and evaluated using the same detection methods. Findings: Compared to the individual sample testing, the sample pooling conserved the sensitivity of the detection in all groups of pooled samples when the Ct value in single test was lower than 33. Conclusion: Specimen pooling may fail to detect positive samples with high Ct values. However, in scarcity of reagents or in population surveys, it could be considered as an alternative method in low prevalence settings. © 2022 Future Medicine Ltd.
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