Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
A Novel Approach to Prepare Insulin-Loaded Poly (Lactic-Co-Glycolic Acid) Microcapsules and the Protein Stability Study Publisher Pubmed



Emami J1 ; Hamishehkar H1, 2 ; Najafabadi AR3, 4 ; Gilani K3 ; Minaiyan M1 ; Mahdavi H5 ; Nokhodchi A6
Authors
Show Affiliations
Authors Affiliations
  1. 1. School of Pharmacy and Pharmaceutical Sciences, Isfahan Pharmaceutical Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Pharmaceutical Technology Laboratory, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  3. 3. Aerosol Research Laboratory, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran
  5. 5. Department of Novel Drug Delivery Systems, Iran Polymer and Petrochemical Institute, Tehran, Iran
  6. 6. Medway School of Pharmacy, Universities of Kent and Greenwich, Chatham Maritime, ME4 4TB, Kent, Central Ave., United Kingdom

Source: Journal of Pharmaceutical Sciences Published:2009


Abstract

The purpose of this study was to propose a new preparation method to fabricate insulin-loaded poly(lactic-coglycolic acid) (PLGA) microparticles satisfying protein loading, release profiles, burst release, and particularly stability of the encapsulated protein. Insulin-loaded microcapsules were produced by a single phase o/o solvent evaporation method. The characteristics of the microcapsules were determined by various methods: the surface morphology and size of microparticles by atomic force microscopy and scanning electron microscopy, insulin crystalinity and drug-polymer interactions by XRD, DSC, and FTIR, chemical integrity and aggregation of insulin using HPLC and SDS-PAGE, the protein secondary structure by far ultraviolet-circular dichroism (CD), the antigenicity activity of insulin with ELISA techniques. PLGA microparticles showed smooth surfaces with microcapsule. Encapsulation efficiency of 51% and constant insulin release rate with initial insulin burst release of 24% was obtained. Encapsulated and released insulin was in the intact form and it was dispersed in crystalline state in the polymer matrix. Ease of manufacturing under mild preparation conditions, high level of drug entrapment, desirable release pattern with relatively low initial burst effect and an ability to preserve protein structure are the advantages which are offered by the developed protein encapsulation method. © 2008 Wiley-Liss, Inc. and the American Pharmacists Association.
Related Docs
View other Related Docs
1. The Effect of Formulation Variables on the Characteristics of Insulin-Loaded Poly(Lactic-Co-Glycolic Acid) Microspheres Prepared by a Single Phase Oil in Oil Solvent Evaporation Method, Colloids and Surfaces B: Biointerfaces (2009)
2. Particle Size Design of Plga Microspheres for Potential Pulmonary Drug Delivery Using Response Surface Methodology, Journal of Microencapsulation (2009)
3. Effect of Citric Acid As Cross-Linking Agent on Insulin Loaded Chitosan Microspheres, Iranian Polymer Journal (English Edition) (2005)
4. Effect of Carrier Morphology and Surface Characteristics on the Development of Respirable Plga Microcapsules for Sustained-Release Pulmonary Delivery of Insulin, International Journal of Pharmaceutics (2010)
5. Pharmacokinetics and Pharmacodynamics of Controlled Release Insulin Loaded Plga Microcapsules Using Dry Powder Inhaler in Diabetic Rats, Biopharmaceutics and Drug Disposition (2010)
6. Nasal Delivery of Insulin Using Chitosan Microspheres, Journal of Microencapsulation (2004)
7. Dissolution Enhancement of Gliclazide Using in Situ Micronization by Solvent Change Method, Powder Technology (2008)
8. Insights Into Whey Protein-Based Carriers for Targeted Delivery and Controlled Release of Bioactive Components, Food Hydrocolloids (2022)
Experts (# of related papers)
View all Related Experts
Mohsen Minaiyan (11)
Jaleh Varshosaz (8)
Other Related Docs
9. In Vitro Study of Polyoxyethylene Alkyl Ether Niosomes for Delivery of Insulin, International Journal of Pharmaceutics (2007)
10. Preparation and Characterization of a Hydroxypropyl Methylcellulose Based Wafer for Simultaneous Delivery of Phenytoin and Insulin As Wound Dressing Material, Pharmaceutical Development and Technology (2022)
11. Preparation and Evaluation of a Controlled Drug Release of Repaglinide Through Matrix Pellets: In Vitro and in Vivo Studies, Journal of Microencapsulation (2014)
12. Protein-Polysaccharide Interactions for the Fabrication of Bioactive-Loaded Nanocarriers: Chemical Conjugates and Physical Complexes, Pharmacological Research (2022)
13. Nasal Delivery of Insulin Using Bioadhesive Chitosan Gels, Drug Delivery: Journal of Delivery and Targeting of Therapeutic Agents (2006)
14. Evaluation of Thermosensitive Chitosan Hydrogel Containing Gefitinib Loaded Cellulose Acetate Butyrate Nanoparticles in a Subcutaneous Breast Cancer Model, International Journal of Pharmaceutics (2022)
15. Development and Validation of a New Robust Rp-Hplc Method for Simultaneous Quantitation of Insulin and Pramlintide in Non-Invasive and Smart Glucose-Responsive Microparticles, Research in Pharmaceutical Sciences (2022)
16. Preparation, Characterization and Antibacterial Activities of Chitosan, N-Trimethyl Chitosan (Tmc) and N-Diethylmethyl Chitosan (Demc) Nanoparticles Loaded With Insulin Using Both the Ionotropic Gelation and Polyelectrolyte Complexation Methods, International Journal of Pharmaceutics (2008)
17. Interaction of Insulin, Cholesterol-Derivatized Mannan, and Carboxymethyl Chitin With Liposomes: A Differential Scanning Calorimetry Study, Research in Pharmaceutical Sciences (2012)
18. Enhancement of Solubility and Antidiabetic Effects of Repaglinide Using Spray Drying Technique in Stz-Induced Diabetic Rats, Pharmaceutical Development and Technology (2017)
19. Alginate-Based Nanocomposite Incorporating Chitosan Nanoparticles: A Dual-Drug Delivery System for Infection Control and Wound Regeneration, Journal of Drug Delivery Science and Technology (2025)
20. Influence of Carrier Particle Size, Carrier Ratio and Addition of Fine Ternary Particles on the Dry Powder Inhalation Performance of Insulin-Loaded Plga Microcapsules, Powder Technology (2010)
21. Preparation, Characterization and Optimization of Glipizide Controlled Release Nanoparticles, Research in Pharmaceutical Sciences (2014)
22. Releasing and Structural/Mechanical Properties of Nano-Particle//Punica Granatum (Pomegranate) in Poly(Lactic-Co-Glycolic) Acid/Fibrin As Nano-Composite Scaffold, Bratislava Medical Journal (2021)
23. Pharmacokinetic Study of Niosome-Loaded Insulin in Diabetic Rats, DARU, Journal of Pharmaceutical Sciences (2011)
24. The Effect of Process Parameters on the Size and Morphology of Poly(D, L-Lactide-Co-Glycolide) Micro/Nanoparticles Prepared by an Oil in Oil Emulsion/Solvent Evaporation Technique, Journal of Applied Polymer Science (2010)