Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Targeted Delivery of Liposomal Ribociclib to Slc7a5 Transporters in Breast Cancer Cells Publisher Pubmed



Afsharzadeh M1 ; Varshosaz J1 ; Mirian M2 ; Hasanzadeh F3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Novel Drug Delivery Systems Research Centre and Department of Pharmaceutics, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, PO Box 81745-359, Isfahan, Iran
  2. 2. Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Novel Drug Delivery Systems Research Centre and Department of Medicinal Chemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Investigational New Drugs Published:2024


Abstract

This study aimed to prepare SLC7A5 transporters targeted liposomes of Ribociclib (RB) by stear(o)yl conjugation of Phe, Asp, Glu amino acids to liposomes as targeting moieties. The liposomes were optimized for their formulations. Cell analysis on two cell lines of MCF-7 and NIH-3T3 were done including; cell viability test by MTT assay, cellular uptake, and cell cycle arrest by flow cytometry. The optimal liposomes showed the particle size of 123.6 ± 1.3 nm, drug loading efficiency and release efficiency of 83.87% ± 1.33% and 60.55% ± 0.46%, respectively. The RB loaded liposomes showed no hemolysis activity. Targeted liposomes increased cytotoxicity on MCF-7 cells more significantly than NIH-3T3 cells. Cell flow cytometry indicated that targeted liposomes uptake was superior to plain (non-targted) liposomes and free drug. Free drug and RB-loaded liposomes interrupted cell cycle in G1. However, amino acid-targeted liposomes arrested cells more than the free drug at this stage. Targeted liposomes reduced cell cycle with more interruption in the G2/M phase compared to the negative control. Graphical abstract: (Figure presented.). © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023.
Related Docs
View other Related Docs
1. Targeted Nanostructured Lipid Carriers for Delivery of Paclitaxel to Cancer Cells: Preparation, Characterization, and Cell Toxicity, Current Drug Delivery (2017)
2. Release Studies on Ciprofloxacin Loaded Non-Ionic Surfactant Vesicles, Avicenna Journal of Medical Biotechnology (2015)
3. Boron Phenyl Alanine Targeted Ionic Liquid Decorated Chitosan Nanoparticles for Mitoxantrone Delivery to Glioma Cell Line, Pharmaceutical Development and Technology (2021)
4. Ciprofloxacin Nano-Niosomes for Targeting Intracellular Infections: An in Vitro Evaluation, Journal of Nanoparticle Research (2013)
5. Co-Delivery of Dasatinib and Mir-30A by Liposomes Targeting Neuropilin-1 Receptors for Triple-Negative Breast Cancer Therapy, Journal of Materials Chemistry B (2024)
6. Gene Delivery by Pei-Nanocomplex Into Breast and Colorectal Tumor Cell Lines, the Impacts of N/P Ratio, Size and Type of the Cell; [Pei-Nanocomplex Tarafindan Meme Ve Kolorektal Tumor Hucre Hatlarinda Gen Dagitimi, N / P Oraninin, Hucrenin Boyutu Ve Turunun Etkileri], Fabad Journal of Pharmaceutical Sciences (2020)
7. A Concise Review on Cancer Treatment Methods and Delivery Systems, Journal of Drug Delivery Science and Technology (2019)
8. Biotin-Encoded Pullulan-Retinoic Acid Engineered Nanomicelles: Preparation, Optimization and in Vitro Cytotoxicity Assessment in Mcf-7 Cells, Indian Journal of Pharmaceutical Sciences (2016)
Experts (# of related papers)
View all Related Experts
Jaleh Varshosaz (17)
Mahboubeh Rostami (7)
Other Related Docs
9. Multifunctional and Stimuli-Responsive Liposomes in Hepatocellular Carcinoma Diagnosis and Therapy, Translational Oncology (2024)
10. Camouflaged Liposomes by 11A4-Nanobody for Co-Delivery of Cisplatin and Nitroxoline in Breast Cancer Tumors: An in Vitro/In Vivo Study, Journal of Drug Delivery Science and Technology (2022)
11. Evaluation of Lipid Nanocarriers in the Form of Sln and Their Application in Drug Delivery, Nanochemistry Research (2023)
12. Docetaxel-Loaded Nanostructured Lipid Carriers Functionalized With Trastuzumab (Herceptin) for Her2-Positive Breast Cancer Cells, Journal of Liposome Research (2018)
13. Development and Optimization of Solid Lipid Nanoparticles of Amikacin by Central Composite Design, Journal of Liposome Research (2010)
14. Cytotoxic Effect of Pluronic F127-Poly (Methyl Vinyl Ether-Alt-Maleic Acid) Micelles Loaded With Doxorubicin on Mcf-7 Cells, Journal of Isfahan Medical School (2013)
15. Biotin Decorated Sunitinib Loaded Nanostructured Lipid Carriers for Tumor Targeted Chemotherapy of Lung Cancer, Journal of Drug Delivery Science and Technology (2019)
16. Delivery of Vinblastine-Containing Niosomes Results in Potent in Vitro/In Vivo Cytotoxicity on Tumor Cells, Drug Development and Industrial Pharmacy (2018)
17. Producing Vesicular Stomatitis Virus G (Vsvg) Protein and Assessment of Its Cytotoxic Activity Against Breast Cancer Cells, Journal of Isfahan Medical School (2015)
18. Lipid Nanocapsules for Imatinib Delivery: Design, Optimization and Evaluation of Anticancer Activity Against Melanoma Cell Line, Iranian Journal of Pharmaceutical Research (2019)
19. Formulation and Characterization of Doxorubicin Nanovesicles, Journal of Vacuum Science and Technology B:Nanotechnology and Microelectronics (2009)
20. Double Flow Focusing Microfluidic-Assisted Based Preparation of Methotrexate–Loaded Liposomal Nanoparticles: Encapsulation Efficacy, Drug Release and Stability, Colloids and Surfaces A: Physicochemical and Engineering Aspects (2021)
21. Cytotoxic Effects of Chitosan/Retinoic Acid/Albumin Targeted Nanoparticles Loaded With Doxorubicin on Human Hepatoma Cells, Journal of Isfahan Medical School (2013)
22. Folic Acid Conjugated Nanoliposomes As Promising Carriers for Targeted Delivery of Bleomycin, Artificial Cells, Nanomedicine and Biotechnology (2018)
23. Preparation and Evaluation of Targeted Albumin Lipid Nanoparticles With Lactobionic Acid for Targeted Drug Delivery of Sorafenib in Hepatocellular Carcinoma, Journal of Drug Delivery Science and Technology (2022)
24. The Methods to Overcome Atp-Binding Cassette Transporters-Mediated Multi-Drug Resistance, Journal of Isfahan Medical School (2013)
25. Lomustine Loaded Superparamagnetic Iron Oxide Nanoparticles Conjugated With Folic Acid for Treatment of Glioblastoma Multiforma (Gbm), Iranian Journal of Pharmaceutical Research (2020)
26. Sucrose Stearate Based Niosomes As an Alternative to Ordinary Vehicles for Efficient Curcumin Delivery, Journal of Food Measurement and Characterization (2022)
27. Targeting Etoposide to Acute Myelogenous Leukaemia Cells Using Nanostructured Lipid Carriers Coated With Transferrin, Nanotechnology (2012)
28. Liposomal Azd5363 Displays Antiproliferation Activities and Induces Apoptosis on Y79 Retinoblastoma Cancer Cells, South Asian Journal of Cancer (2024)
29. Plant Protein-Based Nanoparticles and Their Biomedical Applications, Nanomaterials and Plant Potential (2019)
30. Preparation, Characterization, and in Vitro Evaluation of Bleomycin-Containing Nanoliposomes, Chemical Biology and Drug Design (2017)
31. Pegylated Trimethylchitosan Emulsomes Conjugated to Octreotide for Targeted Delivery of Sorafenib to Hepatocellular Carcinoma Cells of Hepg2, Journal of Liposome Research (2019)
32. Lhrh Targeted Chonderosomes of Mitomycin C in Breast Cancer: An in Vitro/ in Vivo Study, Anti-Cancer Agents in Medicinal Chemistry (2019)
33. Cationic Okra Gum Coated Nanoliposomes As a Ph-Sensitive Carrier for Co-Delivery of Hesperetin and Oxaliplatin in Colorectal Cancers, Pharmaceutical Development and Technology (2022)
34. Redox Responsive Polymeric Micelles of Gellan Gum/Abietic Acid for Targeted Delivery of Ribociclib, International Journal of Biological Macromolecules (2022)