Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Effect of Human Sperm Mtt Viability Test on Outcome of Intracytoplasmic Sperm Injection



Aboutorabi R1 ; Esfahani MHN2, 3, 4, 5 ; Baharvand H4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Anatomy Department, Faculty of Medical Sciences, Isfahan Medical University, Iran
  2. 2. Embryology Department, Royan Institute, Tehran, Iran
  3. 3. Isfahan Fertility and Infertility Center, Iran
  4. 4. Stem Cells Department, Royan Institute, Tehran, Iran
  5. 5. Embryology and Stem Cells Department, Royan Institute, Tehran, P.O.Box: 19395-4644, Iran

Source: Yakhteh Published:2006

Abstract

Introduction: The aim of this study was to evaluate the effect of human sperm MTT viability assay on outcome of intracytoplasmic sperm injection. MTT is a tetrazolium salt, routinely used for cell proliferation and cytotoxicity assays. Material and Methods: 50μl of processed semen was treated with MTT solution, while the remaining used as the control. Meanwhile, 109 donated human oocytes (metaphase II) obtained from 12 patients were divided into two groups. Fifty five oocytes were injected using MTT positive sperms, while 54 oocytes were injected with sperms from the control group. Then the injected oocytes were cultured and observed at 18, 42, 66, 90, and 114 hours pos-ICSI. Finally, the fertilization and embryo development rates were compared in both groups. Results: No significant differences were observed between fertilization and embryo development rates in the MTT and control groups. Conclusion: After approving that the MTT is not cytotoxic or teratogenic effects, the sperm MTT viability assay may be useful for ICSI in patients with absolute or severe asthenospermia or in patients with highly deformed sperm tails.
Related Docs
View other Related Docs
1. Sperm Mtt Viability Assay: A New Method for Evaluation of Human Sperm Viability, Journal of Assisted Reproduction and Genetics (2002)
2. Artificial Oocyte Activation in Severe Teratozoospermia Undergoing Intracytoplasmic Sperm Injection, Fertility and Sterility (2008)
3. Relationship Between Protamine Deficiency With Fertilization Rate and Incidence of Sperm Premature Chromosomal Condensation Post-Icsi, Andrologia (2004)
4. Relationship Between Sperm Parameters and Intracytoplasmic Sperm Injection Outcome, Middle East Fertility Society Journal (2015)
5. Evaluation of Zeta and Ha-Binding Methods for Selection of Spermatozoa With Normal Morphology, Protamine Content and Dna Integrity, Andrologia (2010)
6. Can Sperm Protamine Deficiency Induce Sperm Premature Chromosomal Condensation?, Andrologia (2006)
7. The Role of Sperm Chromatin Anomalies on the Outcome of Assisted Reproductive Techniques, Yakhteh (2006)
8. Evaluation of Sperm Selection Procedure Based on Its Binding Ability to Hyaluronic Acid on Icsi Outcome, Journal of Isfahan Medical School (2008)
Experts (# of related papers)
View all Related Experts
Shahnaz Razavi (25)
Mohammad Mardani (14)
Other Related Docs
9. Can Zeta Sperm Selection Method, Recover Sperm With Higher Dna Integrity Compare to Density Gradient Centrifugation?, Iranian Journal of Reproductive Medicine (2009)
10. Effect of Human Sperm Chromatin Anomalies on Fertilization Outcome Post-Icsi, Andrologia (2003)
11. Evaluation of Sperm Selection Procedure Based on Hyaluronic Acid Binding Ability on Icsi Outcome, Journal of Assisted Reproduction and Genetics (2008)
12. Effects of Failed Oocyte Activation and Sperm Protamine Deficiency on Fertilization Post-Icsi, Reproductive BioMedicine Online (2007)
13. Rate of Cumulus Cell Apoptosis From Fertilized and Unfertilized Oocytes and Acid Phosphatase Levels in Follicular Fluids After Intracytopasmic Sperm Injection, Iranian Biomedical Journal (2005)
14. Treatment Outcome Following Intracytoplasmic Injection of Sperm Retrieved From Ejaculate, Epididymis, or Testis of Infertile Men, Archives of Iranian Medicine (2004)
15. Effects of Temperature and Storage Time on the Motility, Viability, Dna Integrity and Apoptosis of Processed Human Spermatozoa, Andrologia (2020)
16. Assessment of the Changing Trend of Different Kinds of Sperm Motility During the Storage of Normozoospermic Men’S Semen Samples at 4-6 ºc, Journal of Isfahan Medical School (2017)
17. Effect of Polyvinyl Pyrrolidone on Sperm Membrane Integrity and Chromatin Status, International Journal of Fertility and Sterility (2008)
18. Evaluation of the Effects of Nicotinic Acid and Folic Acid on Morphology, Membrane Integrity and Quality of Sperm Chromatin Concentration in Normozoospermic Men During Cryopreservation., Scientific Journal of Kurdistan University of Medical Sciences (2022)
19. Corifollitropin-Α Compared to Daily R-Fsh in for Patients Undergoing Intracytoplasmic Sperm Injection: Clinical Trial Study, International Journal of Reproductive BioMedicine (2019)
20. Effect of Protamine-2 Deficiency on Icsi Outcome, Reproductive BioMedicine Online (2004)
21. The Effects of Main Semen Parameters on Intra-Uterine Insemination (Iui) Outcome, Journal of Isfahan Medical School (2013)
22. Does Folate Deficiency Alter Sperm Parameters in Infertile Males?, Immunopathologia Persa (2024)
23. The Predictive Value of Pronuclear Morphology Screening on Embryo Development and Pregnancy Outcome in Art Cycles, Middle East Fertility Society Journal (2008)
24. Effects of Biotin and Folic Acid on Motility, Viability, Morphology, Chromatin Density and Integrity of Cryopreserved and Thawed Sperm in Normozoospermic Men, Scientific Journal of Kurdistan University of Medical Sciences (2021)
25. A Survey on Main Semen Parameters in Natural Pregnancy and Intrauterine Insemination: Are There Any Significant Differences?, American Journal of Men's Health (2018)
26. Effect of Sperm Dna Damage and Sperm Protamine Deficiency on Fertilization and Embryo Development Post-Icsi, Reproductive BioMedicine Online (2005)
27. Comparing the Roles of Sperm Chromatin Integrity and Apoptosis in Intrauterine Insemination Outcomes of Couples With Mild Male and Female Factor Infertility, Journal of Reproduction and Infertility (2014)
28. The Methylation Status of Gata3 Potentially Predicts the Outcomes of Assisted Reproductive Technologies, Human Fertility (2023)
29. Comparison Between Open and Closed Systems for Vitrification of Individual Sperm: Assessing Morphometric Measurements and Chromatin Integrity, Middle East Fertility Society Journal (2024)
30. Lack of Anti-Paternal Cytotoxic Antibody Production in the Presence of Spermatozoa in Recurrent Spontaneous Abortion Couples in Contrast to Fertile Couples: A Pilot Study, Clinical and Experimental Obstetrics and Gynecology (2021)
31. Can the Decreased Expression of Human Leukocyte Antigen Class I and Ii by Spermatozoa Lead to Recurrent Spontaneous Abortion?, Iranian Journal of Pathology (2020)
32. The Correlation Between Sperm Dna Fragmentation and the Expression of Long Non-Coding Rna Hotair in Infertile Men, Journal of Isfahan Medical School (2021)
33. Assessment of Adiponectin and Sperm Function Parameters in Obese and Non-Obese: A Comprehensive Study, Cell Journal (2022)
34. The Relationship of Oocyte and Sperm Membrane Binding Protein With the Success of Intrauterine Insemination, Journal of Isfahan Medical School (2014)
35. Influence of Sperm Chromatin Anomalies on Assisted Reproductive Technology Outcome, Fertility and Sterility (2009)
36. Credibility of Chromomycin A3 Staining in Prediction of Fertility, International Journal of Fertility and Sterility (2009)
37. Effects of Main Semen Parameters on Intrauterian Insemination (Iui) Outcome, Journal of Isfahan Medical School (2010)
38. Effect of Zinc on Spermatogenesis and Sperm Chromatin Condensation in Bleomycin, Etoposide, Cisplatin Treated Rats, Cell Journal (2019)
39. Gonadotropins in Infertile Men With Idiopathic Hypogonadotropic Hypogonadism, International Journal of Fertility and Sterility (2008)
40. Selection of Sperm Based on Combined Density Gradient and Zeta Method May Improve Icsi Outcome, Human Reproduction (2009)
41. Failed Fertilization After Icsi and Spermiogenic Defects, Fertility and Sterility (2008)
42. Effects of Low Doses of Exogenous Thymoquinone on Sperm Motility and Viability of Normozoospermic Men, Journal of Isfahan Medical School (2014)
43. Investigating the Correlation Between Ubiquitination With Motility, Morphology, and Dna Methylation in Rat Sperm, Journal of Isfahan Medical School (2017)
44. The Effect of Consecutive Ejaculation on the Sperm Parameters in the Oligo-Astheno-Teratozoospermia (Oat) Men, Zygote (2025)
45. Effect of Preservation of Human Semen Sample at 4–6 and 25 °C on Sperm Motility, Cell and Tissue Banking (2018)
46. Efficiency of Sil-Select and Percoll to Recover Spermatozoa With Normal Chromatin and Morphology and the Effect of These Parameters on Fertilization, Embryo Quality and Cleavage Score, Middle East Fertility Society Journal (2003)
47. Effect of Vitamin D Supplementation on Intracytoplasmic Sperm Injection Outcomes: A Randomized Double-Blind Placebo-Controlled Trial, International Journal of Fertility and Sterility (2019)
48. Relation Between Different Human Sperm Nuclear Maturity Tests and in Vitro Fertilization, Journal of Assisted Reproduction and Genetics (2001)
49. Scsa Results Correlated With Rate of Motility Reduction After Ejaculation in Asthenozoospermia, Andrologia (2019)
50. The Effect of 24 Hours Delay in Oocyte Maturation Triggering in Ivf/Icsi Cycles With Antagonist Protocol and Not-Elevated Progesterone: A Randomized Control Trial, International Journal of Reproductive BioMedicine (2017)