Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Growth Inhibitory Effect of Resveratrol and Gallic Acid on Prostate Cancer Cell Lines Through the Alteration of Oxidative Stress Balance: The Interplay Between Nrf2, Ho-1, and Bach1 Genes Publisher Pubmed



Moghadam D1 ; Zarei R1 ; Rostami A2 ; Samarenajaf M1 ; Ghojoghi R3 ; Savardashtaki A4 ; Jafarinia M5 ; Vakili S6 ; Irajie C4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Autophagy Research Center, Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  2. 2. Department of Internal Medicine, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  4. 4. Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
  5. 5. Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  6. 6. Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Source: Anti-Cancer Agents in Medicinal Chemistry Published:2024


Abstract

Background: The association between oxidative stress and prostate cancer (PC) has been demonstrated both epidemiologically and experimentally. Balance in reactive oxygen species (ROS) levels depends on multiple factors, such as the expression of Nrf2, HO-1, and BACH1 genes. Natural polyphenols, such as resveratrol (RSV) and gallic acid (GA), affect cellular oxidative profiles. Objective: The present study investigated the possible effects of GA and RSV on the oxidative profiles of PC3 and DU145 cells, as well as Nrf2, HO-1, and BACH1 gene expression to achieve an understanding of the mechanisms involved. Methods: PC3 and DU145 cells were treated with ascending concentrations of RSV and GA for 72 h. Then cell growth and mRNA expression of Nrf2, HO-1, and BACH1 genes were analyzed by real-time PCR. Various spec-trophotometric analyses were performed to measure oxidative stress markers. Results: RSV and GA significantly decreased the growth of PC3 and DU145 cells compared to the control group in a concentration-dependent manner. RSV and GA also decreased ROS production in PC3 cells, but in DU145 cells, only the latter polyphenol significantly decreased ROS content. In addition, RSV and GA had ameliorating effects on SOD, GR, GPX, and CAT activities and GSH levels in both cell lines. Also, RSV and GA induced HO-1 and Nrf2 gene expression in both cell lines. BACH1 gene expression was induced by RSV only at lower concentrations, in contrast to GA in both cell lines. Conclusion: Our data suggest that RSV and GA can prevent the growth of prostate cancer cells by disrupting oxidative stress-related pathways, such as changes in Nrf2, HO-1, and BACH1 gene expression. © 2024 Bentham Science Publishers.
Related Docs
View other Related Docs
1. Nrf-Mediated Autophagy and Upr: Exploring New Avenues to Overcome Cancer Chemo-Resistance, European Journal of Pharmacology (2025)
2. How Gallic Acid Regulates Molecular Signaling: Role in Cancer Drug Resistance, Medical Oncology (2023)
3. Enhancing Prostate Cancer Cells’ Sensitivity to Flutamide by Resveratrol: An In-Vitro Study, Tissue and Cell (2025)
4. A Comparison of Thiazolyl Blue (Mtt) Versus Sulforhodamine B (Srb) Assay in Assessment of Antiproliferative Effect of Bromelain on 4T1, Ags, and Pc3 Cancer Cell Lines, Journal of Isfahan Medical School (2017)
5. Natural Products As Non-Covalent and Covalent Modulators of the Keap1/Nrf2 Pathway Exerting Antioxidant Effects, European Journal of Medicinal Chemistry (2024)
6. Bladder Cancer: Total Antioxidant Capacity and Pharmacotherapy With Vitamin-E, International Urology and Nephrology (2020)
7. Belinostat Effects on Expression of Rbm5 Tumor Suppressor Gene and Inhibits Prostate Cancer Cell Line (Pc3) Proliferation, Electronic Journal of General Medicine (2018)
8. Expression Assay of Pten, Cdk4, and Mtor Genes and Cell Proliferation in Breast Cancer Cell Line (T47d) Under Grape Seed Extract Treatment, Journal of Isfahan Medical School (2022)
Experts (# of related papers)
View all Related Experts
Zahra Tolou-Ghamari (2)
Daryoush Shahbazi-Gahrouei (1)
Other Related Docs
9. Jpx and Linc00641 Ncrnas Expression in Prostate Tissue: A Case-Control Study, Research in Pharmaceutical Sciences (2021)
10. Resveratrol Enhances Sensitivity of Renal Cell Carcinoma to Tivozanib: An In-Vitro Study, Tissue and Cell (2024)
11. Structure and Function of Fus Gene in Prostate Cancer, Bratislava Medical Journal (2018)
12. The Tangled Web of Dyslipidemia and Cancer: Is There Any Association?, Journal of Research in Medical Sciences (2022)
13. Anticancer Activity of Zinc Oxide Nanoparticles on Prostate and Colon Cancer Cell Line, Toxicology Research (2024)
14. Association of Sod2 Rs2758339, Rs5746136 and Rs2842980 Polymorphisms With Increased Risk of Breast Cancer: A Haplotype-Based Case–Control Study, Genes and Genomics (2023)
15. The Modulatory Effects of Two Bioflavonoids, Quercetin and Thymoquinone on the Expression Levels of Dna Damage and Repair Genes in Human Breast, Lung and Prostate Cancer Cell Lines, Pathology Research and Practice (2022)
16. On the Occasion of World Cancer Day 2015; the Possibility of Cancer Prevention or Treatment With Antioxidants: The Ongoing Cancer Prevention Researches, International Journal of Preventive Medicine (2015)
17. Comprehensive Analysis of the Prognostic Value of Glutathione S-Transferases Mu Family Members in Breast Cancer, Cell Biology International (2024)
18. Nrf2 Signaling Pathway in Trace Metal Carcinogenesis: A Cross-Talk Between Oxidative Stress and Angiogenesis, Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology (2022)
19. A Quick Review of Redox State in Cancer: Focus to Bladder, The Gulf journal of oncology (2020)
20. The Effect of Ganoderma Lucidum Polysaccharide Extract on Sensitizing Prostate Cancer Cells to Flutamide and Docetaxel: An in Vitro Study, Scientific Reports (2023)
21. Metformin and Aspirin: Anticancer Effects on A549 and Pc3 Cancer Cells and the Mechanisms of Action, Toxicology Research (2023)