Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Inflammatory Responses of Women With Polycystic Ovary Syndrome in Vitro Differ From Healthy Women Publisher



Rezayat F1, 2 ; Hajiaghayi M1 ; Ghasemi N1 ; Mesdaghi M1 ; Tehrani FR3 ; Mosaffa N1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Reproductive Endocrinology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: International Journal of Molecular and Cellular Medicine Published:2023


Abstract

Polycystic ovary syndrome is a low-grade inflammatory state with increased serum levels of TNF-α. The present study has compared the inflammatory responses to breast cancer cell lines in women with PCOS with healthy women. Peripheral blood mononuclear cells (PBMCs) isolated from 50 women with PCOS and 50 healthy controls were cultured in the trans-well co-culture system. These cells were stimulated with two distinct breast cancer cell lines. The proliferation of PBMCs, CD3+CD8+T cell percentages, and tumor necrosis factor-alpha (TNFα) concentration were evaluated after 48 and 72 hours of incubation. TNF-α concentration and the proliferation rate of PBMCs after 48 hours of incubation significantly increased in the PCOS group. However, after 72 hours, TNF-α secretion significantly decreased in the PCOS group. The ability of PBMCs to produce TNF-α decreased gradually in women with PCOS. When the effects of low-grade inflammation and endocrine conditions on the cells decrease, the inability of PBMCs to create an inflammatory response will be altered. © The Author(s). Publisher: Babol University of Medical Sciences
Related Docs
View other Related Docs
1. Effects of Probiotic Supplementation on Hormonal and Clinical Outcomes of Women Diagnosed With Polycystic Ovary Syndrome: A Double-Blind, Randomized, Placebo-Controlled Clinical Trial, Journal of Functional Foods (2022)
2. The Effects of Curcumin Supplementation on Glycemic Status, Lipid Profile and Hs-Crp Levels in Overweight/Obese Women With Polycystic Ovary Syndrome: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial, Complementary Therapies in Medicine (2019)
3. Dash Diet, Insulin Resistance, and Serum Hs-Crp in Polycystic Ovary Syndrome: A Randomized Controlled Clinical Trial, Hormone and Metabolic Research (2015)
4. Comparison of the Metabolic Parameters and Androgen Level of Umbilical Cord Blood in Newborns of Mothers With Polycystic Ovary Syndrome and Controls, Journal of Research in Medical Sciences (2012)
5. Chronic Low-Grade Inflammation: Etiology and Its Effects, Journal of Isfahan Medical School (2016)
6. Cardiometabolic Risk Factors in Polycystic Ovary Syndrome, Pakistan Journal of Medical Sciences (2012)
7. Influence of Ω-3 Fatty Acid Eicosapentaenoic Acid on Igf-1 and Cox-2 Gene Expression in Granulosa Cells of Pcos Women, Iranian Journal of Reproductive Medicine (2015)
8. Omega-3 Supplementation Effects on Polycystic Ovary Syndrome Symptoms and Metabolic Syndrome, Journal of Research in Medical Sciences (2017)
Experts (# of related papers)
View all Related Experts
Hatav GhasemiTehrani (3)
Roya Kelishadi (2)
Other Related Docs
9. Calcium Plus Vitamin D Supplementation Influences Biomarkers of Inflammation and Oxidative Stress in Overweight and Vitamin D-Deficient Women With Polycystic Ovary Syndrome: A Randomized Double-Blind Placebo-Controlled Clinical Trial, Clinical Endocrinology (2015)
10. The Effect of Concentrated Pomegranate Juice on Biomarkers of Inflammation, Oxidative Stress, and Sex Hormones in Overweight and Obese Women With Polycystic Ovary Syndrome: A Randomized Controlled Trial, Phytotherapy Research (2023)
11. Diagnostic Value of Prostate-Specific Antigen in Women With Polycystic Ovary Syndrome, Journal of Research in Medical Sciences (2011)
12. The Association Between Dietary Antioxidants, Oxidative Stress Markers, Abdominal Obesity and Poly-Cystic Ovary Syndrome: A Case Control Study, Journal of Obstetrics and Gynaecology (2020)
13. Estrogen Attenuates Leptin-Induce Mrna Expression of Tumour Necrosis Factor Alpha in Human Mononuclear Cells, Biochemical and Cellular Archives (2012)
14. Omega-3 and Vitamin E Co-Supplementation Can Improve Antioxidant Markers in Obese/Overweight Women With Polycystic Ovary Syndrome, International Journal for Vitamin and Nutrition Research (2020)
15. Metformin-Therapy Effects in 50 Clomiphene Citrate Resistant Pcos Patients, Journal of Medical Sciences (2006)
16. Evaluation of Serum Prolactin Levels in Patients With Polycystic Ovary Syndrome, Journal of Isfahan Medical School (2012)
17. Serum Prostate-Specific Antigen Level in Women With Polycystic Ovary Syndrome: A Systematic Review and Meta-Analysis, Hormone and Metabolic Research (2019)
18. A Meta-Analysis of Effects of Vitamin E Supplementation Alone and in Combination With Omega-3 or Magnesium on Polycystic Ovary Syndrome, Scientific Reports (2022)
19. Effects of Inulin-Type Fructans With Different Degrees of Polymerization on Inflammation, Oxidative Stress and Endothelial Dysfunction in Women With Polycystic Ovary Syndrome: A Randomized, Double-Blind, Placebo-Controlled Trial, Clinical Endocrinology (2022)