Investigation of Cgra and Cyp51a Gene Alternations in Aspergillus Fumigatus Strains Exposed to Kombucha Fermented Tea

Investigation of Cgra and Cyp51a Gene Alternations in Aspergillus Fumigatus Strains Exposed to Kombucha Fermented Tea Publisher

Nazemi L¹ ; Hashemi SJ¹ ; Ghazvini RD¹ ; Saeedi M^{2, 3} ; Khodavaisy S¹ ; Barac A⁵ ; Modiri M¹ ; Dana MA¹ ; Shahrabadi ZZ¹ ; Rezaie S^{1, 4}

Show Affiliations

1. Division of Molecular Biology, Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2. Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3. Persian Medicine and Pharmacy Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
5. Clinic for Infectious and Tropical Diseases, Clinical Center of Serbia, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

Source: Current Medical Mycology Published:2019

Abstract

Background and Purpose: Aspergillus fumigatus is one of the most common opportunistic fungus, which causes infection in immunocompromised and neutropenic patients. The current guidelines recommend voriconazole as the initial therapeutic and prophylactic agent for almost all cases, especially in patients with organ transplants, which leads to increased medication resistance in A. fumigatus. The aim of the present study was to evaluate the antifungal activity and effect of kombucha as a natural compound on A. fumigatus growth, as well as on the expression of cgrA and cyp51A genes. Materials and Methods: A panel of 15 A. fumigatus strains with two quality controls of CM237 and CM2627 as susceptible and resistant strains were obtained from Tehran Medical Mycology Laboratory, Tehran,Iran(TMML).Antifungal susceptibility testing assay was performed according to the Clinical and Laboratory Standards Institute (CLSI) M38-A2 document. Moreover, the mycelial dry weight of the fungus was calculated before and after being treated with kombucha. In addition, the quantitative changes in the expression of cgrA and cyp51A genes were analyzed by real-time polymerase chain reaction (real-time PCR) technique. Results: In the present study, the minimum inhibitory concentration ranges of kombucha were measured at 6,170 and 12,300 μg/mL for ten A. fumigatus azole-susceptible strains and 24,700 μg/mL for five A. fumigatus resistant strains. Moreover, changes in mycelial dry weight under kombucha treatment conditions underwent a significant reduction (P≤0.05). A coordinate down-regulation of expression in cgrA and cyp51A genes was observed in all azole-susceptible and -resistant A. fumigatus strains, after treating the fungus with different concentrations of kombucha (P≤0.05). Conclusion: According to the obtained results, kombucha as a natural antioxidant, can exert inhibitory effects against the growth and expression of some genes in A. fumigatusstrains. Copyright © 2019, Published by Mazandaran University of Medical Sciences on behalf of Iranian Society of Medical Mycology and Invasive Fungi Research Center.

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Sadegh Khodavaisy (9)

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Style	Citing Format
MLA	Nazemi L, et al.. "Investigation of Cgra and Cyp51a Gene Alternations in Aspergillus Fumigatus Strains Exposed to Kombucha Fermented Tea." Current Medical Mycology, vol. 5, no. 3, 2019, pp. 36-42.
APA	Nazemi L, Hashemi SJ, Ghazvini RD, Saeedi M, Khodavaisy S, Barac A, Modiri M, Dana MA, Shahrabadi ZZ, Rezaie S (2019). Investigation of Cgra and Cyp51a Gene Alternations in Aspergillus Fumigatus Strains Exposed to Kombucha Fermented Tea. Current Medical Mycology, 5(3), 36-42.
Chicago	Nazemi L, Hashemi SJ, Ghazvini RD, Saeedi M, Khodavaisy S, Barac A, Modiri M, Dana MA, Shahrabadi ZZ, Rezaie S. "Investigation of Cgra and Cyp51a Gene Alternations in Aspergillus Fumigatus Strains Exposed to Kombucha Fermented Tea." Current Medical Mycology 5, no. 3 (2019): 36-42.
Harvard	Nazemi L et al. (2019) 'Investigation of Cgra and Cyp51a Gene Alternations in Aspergillus Fumigatus Strains Exposed to Kombucha Fermented Tea', Current Medical Mycology, 5(3), pp. 36-42.
Vancouver	Nazemi L, Hashemi SJ, Ghazvini RD, Saeedi M, Khodavaisy S, Barac A, et al.. Investigation of Cgra and Cyp51a Gene Alternations in Aspergillus Fumigatus Strains Exposed to Kombucha Fermented Tea. Current Medical Mycology. 2019;5(3):36-42.
BibTex	@article{ author = {Nazemi L and Hashemi SJ and Ghazvini RD and Saeedi M and Khodavaisy S and Barac A and Modiri M and Dana MA and Shahrabadi ZZ and Rezaie S}, title = {Investigation of Cgra and Cyp51a Gene Alternations in Aspergillus Fumigatus Strains Exposed to Kombucha Fermented Tea}, journal = {Current Medical Mycology}, volume = {5}, number = {3}, pages = {36-42}, year = {2019} }
RIS	TY - JOUR AU - Nazemi L AU - Hashemi SJ AU - Ghazvini RD AU - Saeedi M AU - Khodavaisy S AU - Barac A AU - Modiri M AU - Dana MA AU - Shahrabadi ZZ AU - Rezaie S TI - Investigation of Cgra and Cyp51a Gene Alternations in Aspergillus Fumigatus Strains Exposed to Kombucha Fermented Tea JO - Current Medical Mycology VL - 5 IS - 3 SP - 36 EP - 42 PY - 2019 ER -

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