Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation Into Azoospermic Torsion Mouse Model

Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation Into Azoospermic Torsion Mouse Model Publisher Pubmed

Malekzadeh M¹ ; Takzaree N¹ ; Toolee H² ; Kazemzadeh S¹ ; Khanmohammadi N¹ ; Solhjoo S³ ; Sadeghiani G¹ ; Shabani M¹ ; Rastegar T¹

Show Affiliations

1. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Anatomy, School of Medicine, Shahroud University of Medical Sciences, Semnan, Iran
3. Department of Anatomy, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Source: Reproductive Sciences Published:2022

Abstract

Preserving the spermatogonial stem cells (SSCs) in long periods of time during the treatment of male infertility using stem cell banking systems and transplantation is an important issue. Therefore, this study was conducted to develop an optimal cryopreservation protocol for SSCs using 10 mM pentoxifylline (PTX) as an antioxidant in basal freezing medium. Testicular torsion—a mouse model for long-term infertility—was used to transplant fresh SSCs (n = 6), fresh SSCs treated with PTX (n = 6), cryopreserved SSCs with basal freezing medium (n = 6), and cryopreserved SSCs treated with PTX (n = 6). Eight weeks after germ cell transplantation, samples were assessed for proliferation, through evaluation of Ddx4 and Id4 markers, and differentiation via evaluation of C‐Kit and Sycp3, Tnp1, Tnp2, and Prm1 markers. According to morphological and flow cytometry results, SSCs are able to form colonies and express Gfra1, Id4, α6‐integrin, and β1‐integrin markers. We found positive influence from PTX on proliferative and differentiative markers in SSCs transplanted to azoospermic mice. In the recipient testis, donor SSCs formed spermatogenic colonies and sperm. Respecting these data, adding pentoxifylline is a practical way to precisely cryopreserve germ cells enriched for SSCs in cryopreservation, and this procedure could become an efficient method to restore fertility in a clinical setup. However, more studies are needed to ensure its safety in the long term. © 2021, Society for Reproductive Investigation.

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Style	Citing Format
MLA	Malekzadeh M, et al.. "Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation Into Azoospermic Torsion Mouse Model." Reproductive Sciences, vol. 29, no. 2, 2022, pp. 526-539.
APA	Malekzadeh M, Takzaree N, Toolee H, Kazemzadeh S, Khanmohammadi N, Solhjoo S, Sadeghiani G, Shabani M, Rastegar T (2022). Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation Into Azoospermic Torsion Mouse Model. Reproductive Sciences, 29(2), 526-539.
Chicago	Malekzadeh M, Takzaree N, Toolee H, Kazemzadeh S, Khanmohammadi N, Solhjoo S, Sadeghiani G, Shabani M, Rastegar T. "Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation Into Azoospermic Torsion Mouse Model." Reproductive Sciences 29, no. 2 (2022): 526-539.
Harvard	Malekzadeh M et al. (2022) 'Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation Into Azoospermic Torsion Mouse Model', Reproductive Sciences, 29(2), pp. 526-539.
Vancouver	Malekzadeh M, Takzaree N, Toolee H, Kazemzadeh S, Khanmohammadi N, Solhjoo S, et al.. Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation Into Azoospermic Torsion Mouse Model. Reproductive Sciences. 2022;29(2):526-539.
BibTex	@article{ author = {Malekzadeh M and Takzaree N and Toolee H and Kazemzadeh S and Khanmohammadi N and Solhjoo S and Sadeghiani G and Shabani M and Rastegar T}, title = {Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation Into Azoospermic Torsion Mouse Model}, journal = {Reproductive Sciences}, volume = {29}, number = {2}, pages = {526-539}, year = {2022} }
RIS	TY - JOUR AU - Malekzadeh M AU - Takzaree N AU - Toolee H AU - Kazemzadeh S AU - Khanmohammadi N AU - Solhjoo S AU - Sadeghiani G AU - Shabani M AU - Rastegar T TI - Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation Into Azoospermic Torsion Mouse Model JO - Reproductive Sciences VL - 29 IS - 2 SP - 526 EP - 539 PY - 2022 ER -

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