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The Modulating Effects of Vitamin D on the Activity of Β-Catenin in the Endometrium of Women With Endometriosis: A Randomized Exploratory Trial Publisher Pubmed



Pazhohan A1 ; Danaeimehrabad S2 ; Mohamadrezaeii Z1 ; Amidi F3 ; Khodarahmian M4 ; Shabani Nashtaei M3 ; Sobhani A3 ; Farajzadeh MA5
Authors
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Authors Affiliations
  1. 1. Department of Midwifery, Urmia Branch, Islamic Azad University, Urmia, Iran
  2. 2. Infertility Center, Academic Center for Education, Culture and Research, East Azarbaijan, Tabriz, Iran
  3. 3. Department of Anatomy, School of medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of ART, Embryology Laboratory, Arash Women’s Hospital, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Engineering Faculty, Near East University, Nicosia, Turkey

Source: Gynecological Endocrinology Published:2021


Abstract

Aims: The aim of this prospective study was to investigate the effects of vitamin D on the expression and activity of β-catenin, as the key molecule of the Wnt/β-catenin signaling pathway, in endometriosis women. Materials and methods: Thirty four infertile women with stage III or IV endometriosis were randomly divided to two groups. The control group received the routine treatment and the treatment group, beside the routine protocol, received 50000 IU vitamin D weekly for 12–14 weeks. Blood and endometrial tissue were collected from both groups before and after the intervention. Protein and Gene expression levels of β-catenin were assessed by Western blotting and Real-Time PCR, respectively. Results: Compared to before intervention, the expression of active form of β-catenin reduced significantly within treatment group (p =.000), in addition, the difference between control and treatment groups (p =.012) was significant after intervention, too. Also, the ratio of active/total form of β-catenin protein expression was significantly decreased within the treatment group at the end of intervention period (p =.000). Conclusions: It seems vitamin D can change the activity of β-catenin protein in the endometrial cells of endometriosis patients. Further studies on the therapeutic potential of vitamin D in modifying the β-catenin activity in endometriosis patients are warranted. Clinical Trial Registration Number: IRCT2015081823678N1 Trial registration date: 29 September 2015. © 2020 Informa UK Limited, trading as Taylor & Francis Group.
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