Non-Invasive Diagnosis of Early-Onset Coronary Artery Disease Based on Cell Type-Specific Gene Expression Analyses

Non-Invasive Diagnosis of Early-Onset Coronary Artery Disease Based on Cell Type-Specific Gene Expression Analyses Publisher Pubmed

Ziaee S¹ ; Boroumand MA² ; Salehi R¹ ; Sadeghian S³ ; Hosseindokht M¹ ; Sharifi M¹

Show Affiliations

1. Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2. Department of Pathology and Laboratory Medicine, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran
3. Research Department, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Biomedicine and Pharmacotherapy Published:2018

Abstract

A non-invasive diagnostic method based on biomarkers related to endothelial and mononuclear cell dysfunction can provide opportunities for screening and early treatment of atherosclerosis. This study aimed to construct a risk scoring model based on clinical risk factors and molecular markers (lncRNA SENCR and CD markers) at single-cell level for early diagnosis of early-onset coronary artery disease (EOCAD). A single-cell expression analysis was performed on peripheral blood mononuclear cell subsets derived from 253 young individuals (Males ≤45 and Females ≤55 years old) in two training and validation sets using FISH-Flow assay. Concurrent quantifications of intracellular SENCR and surface/intracellular CD31, CD146, CD45 and CD14 in mononuclear cell fractions (Circulating endothelial cell, Monocyte and Lymphocyte) showed a significant reduction in intra-CEC SENCR, increased in intra-monocyte SENCR and also increased surface/intracellular CD146 and CD14 in patients with EOCAD as compared to the controls. Altered biomarkers were combined together as a risk scoring model. The ROC curve analysis on the combination model showed a high-performance in the distinction of our patients with EOCAD and healthy controls. A positive correlation between SENCR and CD14 in monocytes led us to find a binding site corresponding to SENCR and CD14 mRNA interaction. Our study suggested that combination of our molecular and clinical factors can be benefit to early diagnosis of EOCAD. CECs in peripheral blood as the novel approach could reflect molecular alteration in vascular endothelium. Bimodal variation in intracellular SENCR at the single-cell transcriptional level suggests that SENCR has cell-specific function(s) in its epigenetic gene regulation mechanisms. © 2018 Elsevier Masson SAS

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Zohreh Heidary (1)

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Style	Citing Format
MLA	Ziaee S, et al.. "Non-Invasive Diagnosis of Early-Onset Coronary Artery Disease Based on Cell Type-Specific Gene Expression Analyses." Biomedicine and Pharmacotherapy, vol. 108, no. , 2018, pp. 1115-1122.
APA	Ziaee S, Boroumand MA, Salehi R, Sadeghian S, Hosseindokht M, Sharifi M (2018). Non-Invasive Diagnosis of Early-Onset Coronary Artery Disease Based on Cell Type-Specific Gene Expression Analyses. Biomedicine and Pharmacotherapy, 108(), 1115-1122.
Chicago	Ziaee S, Boroumand MA, Salehi R, Sadeghian S, Hosseindokht M, Sharifi M. "Non-Invasive Diagnosis of Early-Onset Coronary Artery Disease Based on Cell Type-Specific Gene Expression Analyses." Biomedicine and Pharmacotherapy 108, no. (2018): 1115-1122.
Harvard	Ziaee S et al. (2018) 'Non-Invasive Diagnosis of Early-Onset Coronary Artery Disease Based on Cell Type-Specific Gene Expression Analyses', Biomedicine and Pharmacotherapy, 108(), pp. 1115-1122.
Vancouver	Ziaee S, Boroumand MA, Salehi R, Sadeghian S, Hosseindokht M, Sharifi M. Non-Invasive Diagnosis of Early-Onset Coronary Artery Disease Based on Cell Type-Specific Gene Expression Analyses. Biomedicine and Pharmacotherapy. 2018;108():1115-1122.
BibTex	@article{ author = {Ziaee S and Boroumand MA and Salehi R and Sadeghian S and Hosseindokht M and Sharifi M}, title = {Non-Invasive Diagnosis of Early-Onset Coronary Artery Disease Based on Cell Type-Specific Gene Expression Analyses}, journal = {Biomedicine and Pharmacotherapy}, volume = {108}, number = {}, pages = {1115-1122}, year = {2018} }
RIS	TY - JOUR AU - Ziaee S AU - Boroumand MA AU - Salehi R AU - Sadeghian S AU - Hosseindokht M AU - Sharifi M TI - Non-Invasive Diagnosis of Early-Onset Coronary Artery Disease Based on Cell Type-Specific Gene Expression Analyses JO - Biomedicine and Pharmacotherapy VL - 108 IS - SP - 1115 EP - 1122 PY - 2018 ER -

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