Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! By
Adipose Tissue-Derived Mesenchymal Stromal Cells Modulate Inflammatory Response and Improve Allograft Islet Transplant in Mice Model of Type 1 Diabetes Publisher Pubmed



B Niknam BAHARE ; Jm Ayenehdeh Jamal MOHAMMADI ; Npd Hosseinkhannazer Nikoo Ph D ; M Vosough MASSOUD ; N Tajik NADER
Authors

Source: Endocrine Research Published:2024


Abstract

Objective: Type 1 diabetes mellitus (T1DM) is an autoimmune disease where immune cells attack insulin-producing beta cells. Islet transplantation is a promising treatment for T1DM. This study aims to evaluate the effects of adipose tissue-derived mesenchymal stem cells (AT-MSCs) in combination with pancreatic islet transplantation using hydrogel. Methods: T1DM mouse model was established using streptozotocin (STZ). Islets and AT-MSCs were co-embedded in a hydrogel and transplanted into diabetic mice. Five groups with six animals in each (control, hydrogel alone, AT-MSCs embedded hydrogel, islet embedded in hydrogel, and islet + AT-MSCs co-imbedded into a hydrogel) were evaluated in terms of blood glucose, insulin levels and serum and lavage cytokine production. Results: During 32 days, blood glucose levels decreased from over 400 mg/dl to less than 150 mg/dl in the transplanted mice. Analysis showed increased transformation growth factor beta (TGF-β1) and IL-4 levels, while IL-17 and IFN-γ levels significantly decreased in the MSC-treated groups. Conclusion: These findings suggest that using AT-MSCs with hydrogel could be a beneficial alternative for enhancing pancreatic islet engraftment and function. © 2024 Elsevier B.V., All rights reserved.
Related Docs
View other Related Docs
1. Immunomodulatory and Protective Effects of Adipose Tissue-Derived Mesenchymal Stem Cells in an Allograft Islet Composite Transplantation for Experimental Autoimmune Type 1 Diabetes, Immunology Letters (2017)
2. Reduction of Marginal Mass Required for Successful Islet Transplantation in a Diabetic Rat Model Using Adipose Tissue–Derived Mesenchymal Stromal Cells, Cytotherapy (2018)
3. Adipose Tissue-Derived Mesenchymal Stem Cells Exert in Vitro Immunomodulatory and Beta Cell Protective Functions in Streptozotocin-Induced Diabetic Mice Model, Journal of Diabetes Research (2015)
Experts (# of related papers)
View all Related Experts
Bagher Larijani (4)
Nima Rezaei (2)
Other Related Docs
4. Microfluidically Fabricated Fibers Containing Pancreatic Islets and Mesenchymal Stromal Cells Improve Longevity and Sustained Normoglycemia in Diabetic Rats, Biofabrication (2023)
5. Mesenchymal Stem Cells: Rising Concerns Over Their Application in Treatment of Type One Diabetes Mellitus, Journal of Diabetes Research (2015)
6. Therapy of Endocrine Disease: Islet Transplantation for Type 1 Diabetes: So Close and yet so Far Away, European Journal of Endocrinology (2015)
7. Investigating the Route of Administration and Efficacy of Adipose Tissue-Derived Mesenchymal Stem Cells and Conditioned Medium in Type 1 Diabetic Mice, Inflammopharmacology (2020)
8. Treatment of Diabetic Mice by Microfluidic System-Assisted Transplantation of Stem Cells-Derived Insulin-Producing Cells Transduced With Mirna, Life Sciences (2021)
9. Introducing a New Experimental Islet Transplantation Model Using Biomimetic Hydrogel and a Simple High Yield Islet Isolation Technique, Iranian Biomedical Journal (2017)
10. Comparison of Human-Induced Pluripotent Stem Cells and Mesenchymal Stem Cell Differentiation Potential to Insulin Producing Cells in 2D and 3D Culture Systems in Vitro, Journal of Cellular Physiology (2020)
11. Vildagliptin Enhances Differentiation of Insulin Producing Cells From Adipose-Derived Mesenchymal Stem Cells, Cell Journal (2019)
12. Assessment of Immune-Alternations and Their Correlations With Therapeutic Outcomes of Transplantation of Autologous Mesenchymal and Allogenic Fetal Stem Cells in Patients With Type 1 Diabetes: A Study Protocol, Journal of Diabetes and Metabolic Disorders (2021)
13. Immunoengineering Biomaterials in Cell-Based Therapy for Type 1 Diabetes, Tissue Engineering - Part B: Reviews (2022)
14. The Generation of Islet-Like Insulin-Producing Cells From Wharton's Jelly-Derived Mesenchymal Stem Cells on the Pes/Fish Gelatin Scaffold, Regenerative Therapy (2024)
15. Skin Wound Healing Following the Spray of Human Abdominal Adipose Tissue-Derived Mesenchymal Stem Cells in Diabetic Male Rat, Koomesh (2022)
16. Generation of High Yield Insulin-Producing Cells (Ipcs) From Various Sources of Stem Cells, Vitamins and Hormones (2021)
17. Insulin Producing Cells Generation by Overexpression of Mir-375 in Adipose-Derived Mesenchymal Stem Cells From Diabetic Patients, Biologicals (2017)
18. Three-Dimensional Nanofiberous Plla/Pcl Scaffold Improved Biochemical and Molecular Markers Hips Cell-Derived Insulin-Producing Islet-Like Cells, Artificial Cells# Nanomedicine and Biotechnology (2018)
19. Investigating the Safety and Efficacy of Hematopoietic and Mesenchymal Stem Cell Transplantation for Treatment of T1dm: A Systematic Review and Meta-Analysis, Systematic Reviews (2022)
20. Administration of Autologous Mesenchymal Stem Cell Transplantation for Treatment of Type 1 Diabetes Mellitus, Iranian Journal of Public Health (2015)