Cytotoxicity Effect of Gold Nanoparticles Against a Rabbit Model of Retinoblastoma



Moradi S¹ ; Mokhtaridizaji M¹ ; Ghasemi F² ; Sheibani S³ ; Amoli FA⁴ Show Affiliations
Source: Koomesh Published:2019

Abstract

Introduction: Retinoblastoma is a malignant tumor in children. The goal of retinoblastoma treatment is to maintain vision and minimize side effects. In this study, the toxicity of the gold nanoparticle safety in vitro was investigated on an eye tumor of retinoblastoma under in vivo conditions. Materials and Methods: For the evaluation of the toxicity of gold nanoparticles using MTT test after 48 hours in a CO2 incubator at 37 ° C, Y79 cell line was administered under in vitro conditions at concentrations of 1.69, 1.75, 3.5, 7, 14 and 28 μg/ml of gold nanoparticles. Safe dose in vitro was extracted. The toxicity of gold nanoparticles in the treatment of retinoblastoma tumor under in vivo conditions in a rabbit animal model using histochemistry analysis and hemoxin-eosin staining, as well as measuring the cross-sectional area of the tumor using ultrasonic sequential image analysis and extraction using software Image J was studied. Results: The results showed that with increasing the concentration of gold nanoparticles, the percentage of live cells in comparison with the control group had a significant difference (p <0.05) at concentrations greater than 1.69 μg/ml and no significant difference (p> 0.05) in the concentration of 1.69 μg/ml. The results indicated that the injection of gold nanoparticles into the rabbit's eye, with retinoblastoma tumor reduced the size of tumor in the rabbit's eye. Conclusion: It is concluded that the toxicity of gold nanoparticles has a therapeutic effect on Y79 cell line in vitro and in the eye of retinoblastoma tumors in vivo. © 2019, Semnan University of Medical Sciences. All rights reserved.