Evaluation of Semi-Nested Pcr Compared With Indirect-Elisa to Diagnose Human Fasciolosis Publisher



Alizadeh G¹ ; Aryaeipour M¹ ; Mohebali M^{1, 2} ; Mowlavi GR¹ ; Raissi V¹ ; Rokni MB^{1, 2}
Source: Iranian Journal of Public Health Published:2022

Abstract

Background: We aimed to compare semi-nested PCR with indirect ELISA to diagnose human fasciolosis. Methods: Overall, 70 serum samples were collected from different areas in Iran suspected for fascioliasis. Indi-viduals were classified based on diagnostic of fascioliasis and habitat in endemic areas. Finally, all serum samples were tested by indirect ELISA (using secretory excretory antigen) and semi-nested PCR (using ITS1 gene). The study was conducted in the School of Publish Health, Tehran University of Medical Sciences, Iran in 2021. Results: Significant differences were found between agreement and similarity of patients' results of indirect ELISA and semi-nested PCR 94.46% and 98.4% respectively (Cohen's kappa ≥0.6; P≤0.05). No cross-reactions were observed with other parasitic diseases (toxocariasis, hydatidosis, strongyloidiasis, toxoplasmosis, cutaneous leishmaniasis, taeniasis and trichinosis). 69.84% of samples were positive by both techniques. In addition, the percentage of agreement and similarity between the results of the two techniques based on habitat in endemic areas was 88.9-100% and 97.7-100%, respectively (Cohen's kappa ≥0.6; P≤0.05). Conclusion: Semi-nested PCR could be a suitable method for following up on patients' treatment and a con-firmatory method for ELISA as for diagnosis of human fascioliasis. © 2022 Alizadeh et al. Published by Tehran University of Medical Sciences.