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Free Radical Scavengers Improve Liver Function But Not Morphological Changes Induced by Reperfusion Injury Publisher Pubmed



Arab HA1 ; Walker NI2 ; Cheung K3, 4 ; Hickman PE4 ; Potter JM4 ; Kadkhodaee M5 ; Roberts MS6, 7
Authors
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Authors Affiliations
  1. 1. Faculty of Veterinary Medicine, University of Tehran, Azadi Street., Tehran, 14155-6453, Iran
  2. 2. Department of Pathology, University of Queensland, Brisbane, 4006, QLD, Australia
  3. 3. Department of Chemical Pathology, Princess Alexandra Hospital, Brisbane, 4102, QLD, Australia
  4. 4. ANU Medical School and ACT Pathology, Canberra, 4005, ACT, Australia
  5. 5. Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Therapeutics Research Centre, University of Queensland, Princess Alexandra Hospital, Brisbane, 4102, QLD, Australia
  7. 7. Sansom Centre for Health Research, University of South Australia, Adelaide, 5000, Australia

Source: Journal of Investigative Surgery Published:2015


Abstract

Objective: Reperfusion injury (RI) is associated with high generation of reactive oxygen species (ROS), but the extent of involvement of these agents in the injury remains controversial. The present study aimed to examine the effectiveness of ROS scavengers against hepatic reperfusion injury in the rat. Methods: The RI was induced in the liver using an isolated slow-flow, reflow perfused rat liver in both anterograde and retrograde perfusion. The effects of gentisic acid, N-acetyl cysteine, and trolox C on the superoxide production, liver function, and morphological changes were examined using different biochemical and histological assays. Results: The hepatic RI caused a significant (p < 0.05) increase in superoxide production and enzyme releases and a decrease in bile flow in both directions. Histological changes induced by RI include apoptosis, necrosis, pale cytoplasm, cell vacuolation, and attenuation of cell cords. Although the production of superoxide in retrograde direction was significantly less than the anterograde, the extent of the injury in the retrograde was greater than the anterograde direction. Pretreatment of the livers with each of the test compounds significantly reduced the release of lactate dehydrogenase and aspartate aminotransferase and improved bile flow in the liver exposed to hypoxia/reperfusion. However, they failed to protect the liver against the structural alterations induced by RI. Conclusion: ROS scavengers can reduce superoxide-induced damage and improve the liver function, but they are not able to prevent the structural changes. It shows that ROS are not the sole causative mechanism of hepatic RI and other mechanisms and mediators may be involved. © 2015 Informa Healthcare USA, Inc.