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Human Amniotic Fluid Derived Mesenchymal Stem Cells Cause an Anti-Cancer Effect on Breast Cancer Cell Line in Vitro Publisher Pubmed



Ghafarzadeh M1 ; Eatemadi A2 ; Fakhravar Z3
Authors
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Authors Affiliations
  1. 1. Assalian Hospital, Center for Obstetrics and Gynecology, Lorestan University of Medical sciences, Khoramabad, Iran
  2. 2. Department of Medical Biotechnology, School of advance Science in Medicine, Tehran University of Medical sciences, Tehran, Iran
  3. 3. Razi Drug applied research center, Lorestan University of Medical sciences, Khoramabad, Iran

Source: Cellular and Molecular Biology Published:2016


Abstract

Human amniotic fluid stem cells (hAFSCs) have the ability to self-renew, and multipotent differentiation into three germ layer cells. We obtained 5 ml amniotic fluid from ten 16-20 week pregnant women undergoing amniocentesis. hAFSCs were isolated from all samples, co-cultured with T47D breast cancer cell line and characterized using flow cytometry and RT-PCR. After 3, 4 and 5 days, T47D and HSFCs viability were evaluated with MTT assay. After 5 days of co-culture T47D cells viability were decreased. Our findings showed that hAFSCs can release soluble factors in cell culture, causing an efficient anticancer effect. © 2016 by the C.M.B. Association. All rights reserved.