Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Effect of Lyophilization on Light Transmission of Amniotic Membrane: A Comparison With Rabbit Cornea



Deihim T1 ; Yazdanpanah G1 ; Niknejad H1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Journal of Kerman University of Medical Sciences Published:2016

Abstract

Background & Aims: Amniotic membrane persists for a long time after ocular transplantation (as corneal substitute) and can affect light transmission (transparency). The aim of this study was to evaluate the transparency of amniotic membrane after freeze-drying (lyophilization) and to compare the results with transparency of rabbit cornea. Methods: Transparency of rabbits’ corneas and fresh and lyophilized AMs from disk and peripheral (out of disk) regions of placenta were measured. The epithelial cells which have stem cell properties were removed with enzymatic digestion. The structure of amniotic membrane was evaluated using H&E, periodic acid-schiff and masson’s trichrome staining, and ultrastructural evaluation of surface was carried out using scanning electron microscope. Results: The transparency of lyophilized amniotic membrane was not different with that of fresh one. The light transmission of amniotic membrane from disk region was less than peripheral region. Removing of the epithelial layer improved the light transmission of the amniotic membrane up to the transparency of rabbit cornea. Scanning electron microscopy revealed that the epithelial cell remnants scatter the light after lyophilization. Conclusion: Based on the results of this study, it is recommended to remove the epithelial layer of the amniotic membrane before lyophilization to increase its transparency amniotic membrane for corneal transplantation and ocular tissue engineering. © 2016, Kerman University of Medical Sciences. All rights reserved.
Related Docs
View other Related Docs
1. Different Light Transmittance of Placental and Reflected Regions of Human Amniotic Membrane That Could Be Crucial for Corneal Tissue Engineering, Cornea (2016)
2. The Effects of Cryopreservation on Angiogenesis Modulation Activity of Human Amniotic Membrane, Cryobiology (2015)
3. How Preparation and Preservation Procedures Affect the Properties of Amniotic Membrane? How Safe Are the Procedures?, Burns (2020)
4. Induction of Apoptosis, Stimulation of Cell-Cycle Arrest and Inhibition of Angiogenesis Make Human Amnion-Derived Cells Promising Sources for Cell Therapy of Cancer, Cell and Tissue Research (2016)
5. Extract of Fetal Membrane Would Inhibit Thrombosis and Hemolysis, Medical Hypotheses (2015)
6. Evaluation of Blood Compatibility of Amnion Epithelial Side Compared to Artificial Vessel (Polytetrafluoroethylene), Journal of Mazandaran University of Medical Sciences (2015)
7. Anticancer Properties of Amniotic Membrane Epithelial Cells, Journal of Global Pharma Technology (2016)
8. Low Ph Preconditioned Amniotic Epithelial Cells for Stem Cell Therapy of Cancer, Physiology and Pharmacology (2016)
Experts (# of related papers)
View all Related Experts
Mehdi Yaseri (2)
Ehsan Taghiabadi (2)
Other Related Docs
9. Evaluating the Effects of Fresh and Cryopreserved Amniotic Membrane on Viability of Hela and Mda-Mb-231 Cancer Cells and Angiogenesis of Rat Aorta Ring, Journal of Isfahan Medical School (2017)
10. Evaluating the Efficacy of Tissue-Engineered Human Amniotic Membrane in the Treatment of Myocardial Infarction, Regenerative Medicine (2019)
11. Decellularized Human Amniotic Membrane: How Viable Is It As a Delivery System for Human Adipose Tissue-Derived Stromal Cells?, Cell Proliferation (2016)
12. Silk Fibroin/Amniotic Membrane 3D Bi-Layered Artificial Skin, Biomedical Materials (Bristol) (2018)
13. Bioinformatics Analysis of Hsp90 in Amniotic Fluid and Amniotic Membrane Cells As a Potential Anticancer Drug Target, Journal of Global Pharma Technology (2016)
14. Design of Experiment, Preparation, and in Vitro Biological Assessment of Human Amniotic Membrane Extract Loaded Nanoparticles, Current Pharmaceutical Biotechnology (2020)
15. Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration Due to Pseudomonas Keratitis in a Rabbit Model, Journal of Ophthalmic and Vision Research (2021)
16. Systematic Review and Meta-Analysis Investigating Autograft Versus Allograft Cultivated Limbal Epithelial Transplantation in Limbal Stem Cell Deficiency, International Ophthalmology (2019)
17. Unrestricted Somatic Stem Cells, As a Novel Feeder Layer: Ex Vivo Culture of Human Limbal Stem Cells, Journal of Cellular Biochemistry (2018)
18. A Randomized Clinical Trial to Evaluate the Usefulness of Amniotic Membrane Transplantation in Bacterial Keratitis Healing, Ocular Surface (2017)
19. Management Strategies of Ocular Chemical Burns: Current Perspectives, Clinical Ophthalmology (2020)
20. Amniotic Membrane Seeded Fetal Fibroblasts As Skin Substitute for Wound Regeneration, Methods in Molecular Biology (2019)
21. Near-Ir Absorbing Quantum Dots Might Be Usable for Growth Factor-Based Differentiation of Stem Cells, Journal of Medical Hypotheses and Ideas (2015)
22. Human Amniotic Epithelial Cells Exert Anti-Cancer Effects Through Secretion of Immunomodulatory Small Extracellular Vesicles (Sev), Cancer Cell International (2022)
23. Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering, Methods in Molecular Biology (2019)
24. Decellularized Human Amniotic Membrane: More Is Needed for an Efficient Dressing for Protection of Burns Against Antibiotic-Resistant Bacteria Isolated From Burn Patients, Burns (2015)
25. Promotion of Excisional Wound Repair by a Menstrual Blood-Derived Stem Cell-Seeded Decellularized Human Amniotic Membrane, Biomedical Engineering Letters (2018)
26. Update Review on Five Top Clinical Applications of Human Amniotic Membrane in Regenerative Medicine, Placenta (2021)