Electrospun Mat With Eyelid Fat-Derived Stem Cells As a Scaffold for Ocular Epithelial Regeneration

Electrospun Mat With Eyelid Fat-Derived Stem Cells As a Scaffold for Ocular Epithelial Regeneration Publisher Pubmed

Momenzadeh D¹ ; Baradaranrafii A² ; Keshel SH³ ; Ebrahimi M⁴ ; Biazar E⁵

Show Affiliations

1. Brain and Spinal Injury Research Center, Tehran University of Medical Sciences, Tehran, Iran
2. Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3. Stem Cell Preparation Unit, Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran
4. Tissue Engineering Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
5. Department of Biomaterials Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

Source: Artificial Cells# Nanomedicine and Biotechnology Published:2017

Abstract

The aim of this study was to develop nanofibrous gelatin substrates for eyelid fat stem cell (EFSC) expansion that can serve as a potential alternative substrate to replace human amniotic membrane. Biocompatibility results indicated that all substrates were highly biocompatible, as EFSCs could favorably attach and proliferate on the nanofibrous surfaces. Microscopic figures showed that the EFSC were firmly anchored to the substrates and were able to retain a normal stem cell phenotype. Immunocytochemistry (ICC) and real time-PCR results revealed change in the expression profile of EFSCs grown on nanofibrous substrates when compared to those grown on control in epithelial induction condition. In addition, electrospun gelatin mats especially oriented scaffold provides not only a milieu supporting EFSCs expansion, but also serves as a useful alternative carrier for ocular surface tissue engineering and could be used as an alternative substrate to amniotic membrane. © 2016 Taylor & Francis.

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Style	Citing Format
MLA	Momenzadeh D, et al.. "Electrospun Mat With Eyelid Fat-Derived Stem Cells As a Scaffold for Ocular Epithelial Regeneration." Artificial Cells# Nanomedicine and Biotechnology, vol. 45, no. 1, 2017, pp. 120-127.
APA	Momenzadeh D, Baradaranrafii A, Keshel SH, Ebrahimi M, Biazar E (2017). Electrospun Mat With Eyelid Fat-Derived Stem Cells As a Scaffold for Ocular Epithelial Regeneration. Artificial Cells# Nanomedicine and Biotechnology, 45(1), 120-127.
Chicago	Momenzadeh D, Baradaranrafii A, Keshel SH, Ebrahimi M, Biazar E. "Electrospun Mat With Eyelid Fat-Derived Stem Cells As a Scaffold for Ocular Epithelial Regeneration." Artificial Cells# Nanomedicine and Biotechnology 45, no. 1 (2017): 120-127.
Harvard	Momenzadeh D et al. (2017) 'Electrospun Mat With Eyelid Fat-Derived Stem Cells As a Scaffold for Ocular Epithelial Regeneration', Artificial Cells# Nanomedicine and Biotechnology, 45(1), pp. 120-127.
Vancouver	Momenzadeh D, Baradaranrafii A, Keshel SH, Ebrahimi M, Biazar E. Electrospun Mat With Eyelid Fat-Derived Stem Cells As a Scaffold for Ocular Epithelial Regeneration. Artificial Cells# Nanomedicine and Biotechnology. 2017;45(1):120-127.
BibTex	@article{ author = {Momenzadeh D and Baradaranrafii A and Keshel SH and Ebrahimi M and Biazar E}, title = {Electrospun Mat With Eyelid Fat-Derived Stem Cells As a Scaffold for Ocular Epithelial Regeneration}, journal = {Artificial Cells# Nanomedicine and Biotechnology}, volume = {45}, number = {1}, pages = {120-127}, year = {2017} }
RIS	TY - JOUR AU - Momenzadeh D AU - Baradaranrafii A AU - Keshel SH AU - Ebrahimi M AU - Biazar E TI - Electrospun Mat With Eyelid Fat-Derived Stem Cells As a Scaffold for Ocular Epithelial Regeneration JO - Artificial Cells# Nanomedicine and Biotechnology VL - 45 IS - 1 SP - 120 EP - 127 PY - 2017 ER -

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