Synthesis of Calcium Phosphate-Zirconia Scaffold and Human Endometrial Adult Stem Cells for Bone Tissue Engineering Publisher Pubmed



Alizadeh A^{1, 3} ; Moztarzadeh F² ; Ostad SN¹ ; Azami M¹ ; Geramizadeh B³ ; Hatam G⁴ ; Bizari D² ; Tavangar SM¹ ; Vasei M¹ ; Ai J^{1, 5} Show Affiliations
Source: Artificial Cells# Nanomedicine and Biotechnology Published:2016

Abstract

To address the hypothesis that using a zirconia (ZrO2)/β-tricalcium phosphate (β-TCP) composite might improve both the mechanical properties and cellular compatibility of the porous material, we fabricated ZrO2/β-TCP composite scaffolds with different ZrO2/β-TCP ratios, and evaluated their physical and mechanical characteristics, also the effect of three-dimensional (3D) culture (ZrO2/β-TCP scaffold) on the behavior of human endometrial stem cells. Results showed the porosity of a ZrO2/β-TCP scaffold can be adjusted from 65% to 84%, and the compressive strength of the scaffold increased from 4.95 to 6.25 MPa when the ZrO2 content increased from 30 to 50 wt%. The cell adhesion and proliferation in the ZrO2/β-TCP scaffold was greatly improved when ZrO2 decreased. Moreover, in vitro study showed that an osteoblasts-loaded ZrO2/β-TCP scaffold provided a suitable 3D environment for osteoblast survival and enhanced bone regeneration. We thus showed that a porous ZrO2/β-TCP composite scaffold has excellent mechanical properties, and cellular/tissue compatibility, and would be a promising substrate to achieve both bone reconstruction and regeneration needed during in vivo study for treatment of large bone defects. © Copyright 2014 Informa Healthcare USA, Inc.