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Developmental Regulation and Lateralisation of the Α7 and Α4 Subunits of Nicotinic Acetylcholine Receptors in Developing Rat Hippocampus Publisher Pubmed



Baradaran R1 ; Khoshdelsarkarizi H1 ; Kargozar S2 ; Hami J3 ; Mohammadipour A1 ; Sadrnabavi A4, 5 ; Peyvandi Karizbodagh M1 ; Kheradmand H6 ; Haghir H1, 5
Authors
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Authors Affiliations
  1. 1. Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  2. 2. Tissue Engineering Research Group (TERG), Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  3. 3. Department of Anatomical Sciences, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran
  4. 4. Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  5. 5. Medical Genetic Research Center (MGRC), School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  6. 6. Hazrat Rasoul Hospital, Tehran University of Medical Sciences, Tehran, Iran

Source: International Journal of Developmental Neuroscience Published:2020


Abstract

The purpose of this study was to describe the distinct regional distribution patterns of expression of the α7 and α4 subunits of nicotinic acetylcholine receptors (nAChRs) and their left-right lateralisation in the rat hippocampus during the first 2 weeks of postnatal (P) development. Eighteen male pups were randomly divided into three groups: P0, P7, and P14. After removing the newborn brains, real-time polymerase chain reaction, western blot, and immunohistochemistry techniques were used to evaluate expression of the receptors. Results indicated that the expression profile of these receptors were time- and spatially dependent. A significant increase was observed in the distribution of α7 and α4 nAChR subunits in the developing rat hippocampus from P0 to P7 (p <.001); however, there was a significant decrease from P7 to P14 (p <.05). As a spatial effect, the highest optical density (OD) was observed in the CA3 and CA2 regions of the hippocampus, while the lowest OD was in the dentate gyrus. Moreover, the distribution of α7 and α4 nAChR subunits in the left hippocampus was significantly higher than their counterparts in the right (p <.05). From these data, the expression patterns of α7 and α4 nAChR subunits exhibited left-right asymmetry in the developing rat hippocampus. © 2020 International Society for Developmental Neuroscience
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