The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder

The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder Publisher Pubmed

Gholami K¹ ; Seyedjafari E² ; Mahdavi FS² ; Naghdipoor M¹ ; Mesbah G^{1, 3} ; Zahmatkesh P¹ ; Akbarzadehmoallemkolaei M⁴ ; Baghdadabad LZ¹ ; Pandian SK⁵ ; Meilika KN⁶ ; Aghamir SMK¹

Show Affiliations

1. Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Biotechnology, College of Science, University of Tehran, Tehran, 1416753955, Iran
3. AshianGanoTeb Biopharmaceutical Company, Golestan University of Medical Sciences, Gorgan, Iran
4. Animal Model Integrated Network (AMIN), Universal Scientific Education and Research Network (USERN), Tehran, Iran
5. Chelsea and Westminster Hospital NHS Foundation Trust, London, United Kingdom
6. Urology Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, 1416753955, NY, United States

Source: Macromolecular Bioscience Published:2024

Abstract

Nanofibrous scaffolds have attracted much attention in bladder reconstruction approaches due to their excellent mechanical properties. In addition, their biological properties can be improved by combination with biological materials. Taking into account the advantages of nanofibrous scaffolds and decellularized extracellular matrix (dECM) in tissue engineering, scaffolds of poly‑L‑lactic acid (PLLA) coated with decellularized human amnion membrane (hAM) or sheep bladder (SB)-derived ECM proteins are developed (amECM-coated PLLA and sbECM-coated PLLA, respectively). The bladder regenerative potential of modified electrospun PLLA scaffolds is investigated in rabbits. The presence of ECM proteins is confirmed on the nanofibers’ surface. Coating the surface of the PLLA nanofibers improves cell adhesion and proliferation. Histological and immunohistochemical evaluations show that rabbits subjected to cystoplasty with a multilayered PLLA scaffold show de novo formation and maturation of the multilayered urothelial layer. However, smooth muscle bundles (myosin heavy chain [MHC] and α-smooth muscle actin [α-SMA] positive) are detected only in ECM-coated PLLA groups. All groups show no evidence of a diverticulumor fistula in the urinary bladder. These results suggest that the biofunctionalization of electrospun PLLA nanofibers with ECM proteins can be a promising option for bladder tissue engineering. Furthermore, hAM can also replace animal-sourced ECM proteins in bladder tissue regeneration approaches. © 2023 Wiley-VCH GmbH.

Style	Citing Format
MLA	Gholami K, et al.. "The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder." Macromolecular Bioscience, vol. 24, no. 3, 2024, pp. -.
APA	Gholami K, Seyedjafari E, Mahdavi FS, Naghdipoor M, Mesbah G, Zahmatkesh P, Akbarzadehmoallemkolaei M, Baghdadabad LZ, Pandian SK, Meilika KN, Aghamir SMK (2024). The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder. Macromolecular Bioscience, 24(3), -.
Chicago	Gholami K, Seyedjafari E, Mahdavi FS, Naghdipoor M, Mesbah G, Zahmatkesh P, Akbarzadehmoallemkolaei M, et al.. "The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder." Macromolecular Bioscience 24, no. 3 (2024): -.
Harvard	Gholami K et al. (2024) 'The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder', Macromolecular Bioscience, 24(3), pp. -.
Vancouver	Gholami K, Seyedjafari E, Mahdavi FS, Naghdipoor M, Mesbah G, Zahmatkesh P, et al.. The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder. Macromolecular Bioscience. 2024;24(3):-.
BibTex	@article{ author = {Gholami K and Seyedjafari E and Mahdavi FS and Naghdipoor M and Mesbah G and Zahmatkesh P and Akbarzadehmoallemkolaei M and Baghdadabad LZ and Pandian SK and Meilika KN and Aghamir SMK}, title = {The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder}, journal = {Macromolecular Bioscience}, volume = {24}, number = {3}, pages = {-}, year = {2024} }
RIS	TY - JOUR AU - Gholami K AU - Seyedjafari E AU - Mahdavi FS AU - Naghdipoor M AU - Mesbah G AU - Zahmatkesh P AU - Akbarzadehmoallemkolaei M AU - Baghdadabad LZ AU - Pandian SK AU - Meilika KN AU - Aghamir SMK TI - The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder JO - Macromolecular Bioscience VL - 24 IS - 3 SP - EP - PY - 2024 ER -

Style

Citing Format

MLA

Gholami K, et al.. "The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder." Macromolecular Bioscience, vol. 24, no. 3, 2024, pp. -.

APA

Gholami K, Seyedjafari E, Mahdavi FS, Naghdipoor M, Mesbah G, Zahmatkesh P, Akbarzadehmoallemkolaei M, Baghdadabad LZ, Pandian SK, Meilika KN, Aghamir SMK (2024). The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder. Macromolecular Bioscience, 24(3), -.

Chicago

Gholami K, Seyedjafari E, Mahdavi FS, Naghdipoor M, Mesbah G, Zahmatkesh P, Akbarzadehmoallemkolaei M, et al.. "The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder." Macromolecular Bioscience 24, no. 3 (2024): -.

Harvard

Gholami K et al. (2024) 'The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder', Macromolecular Bioscience, 24(3), pp. -.

Vancouver

Gholami K, Seyedjafari E, Mahdavi FS, Naghdipoor M, Mesbah G, Zahmatkesh P, et al.. The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder. Macromolecular Bioscience. 2024;24(3):-.

BibTex

@article{ author = {Gholami K and Seyedjafari E and Mahdavi FS and Naghdipoor M and Mesbah G and Zahmatkesh P and Akbarzadehmoallemkolaei M and Baghdadabad LZ and Pandian SK and Meilika KN and Aghamir SMK}, title = {The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder}, journal = {Macromolecular Bioscience}, volume = {24}, number = {3}, pages = {-}, year = {2024} }

RIS

TY - JOUR
AU - Gholami K
AU - Seyedjafari E
AU - Mahdavi FS
AU - Naghdipoor M
AU - Mesbah G
AU - Zahmatkesh P
AU - Akbarzadehmoallemkolaei M
AU - Baghdadabad LZ
AU - Pandian SK
AU - Meilika KN
AU - Aghamir SMK
TI - The Effect of Multilayered Electrospun Plla Nanofibers Coated With Human Amnion or Bladder Ecm Proteins on Epithelialization and Smooth Muscle Regeneration in the Rabbit Bladder
JO - Macromolecular Bioscience
VL - 24
IS - 3
SP -
EP -
PY - 2024
ER -

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