Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Inhibition of Hif-1Α/Ep4 Axis by Hyaluronate-Trimethyl Chitosan-Spion Nanoparticles Markedly Suppresses the Growth and Development of Cancer Cells Publisher Pubmed



Karpisheh V1, 2 ; Fakkari Afjadi J3 ; Nabi Afjadi M4 ; Haeri MS1 ; Abdpoor Sough TS2 ; Heydarzadeh Asl S2 ; Edalati M5 ; Atyabi F6 ; Masjedi A2 ; Hajizadeh F7 ; Izadi S8 ; Mirzazadeh Tekie FS6 ; Hajiramezanali M9 ; Sojoodi M10 Show All Authors
Authors
  1. Karpisheh V1, 2
  2. Fakkari Afjadi J3
  3. Nabi Afjadi M4
  4. Haeri MS1
  5. Abdpoor Sough TS2
  6. Heydarzadeh Asl S2
  7. Edalati M5
  8. Atyabi F6
  9. Masjedi A2
  10. Hajizadeh F7
  11. Izadi S8
  12. Mirzazadeh Tekie FS6
  13. Hajiramezanali M9
  14. Sojoodi M10
  15. Jadidiniaragh F2, 11
Show Affiliations
Authors Affiliations
  1. 1. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
  2. 2. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  3. 3. Department of Biology, Faculty of Science, Islamic Azad University, Ashkezar Branch, Yazd, Iran
  4. 4. Department of Biochemistry, Faculty of Biological Sciences, University of Tarbiat Modares, Tehran, Iran
  5. 5. Department of Laboratory Sciences, Paramedical Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
  6. 6. Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  8. 8. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  9. 9. Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  10. 10. Division of Surgical Oncology, Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, United States
  11. 11. Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

Source: International Journal of Biological Macromolecules Published:2021


Abstract

Increased expression of Hypoxia-inducible factor-1α (HIF-1α) in the tumor microenvironment, mainly due to tumor growth, plays a major role in the growth of cancer. Tumor cells induce the expression of cyclooxygenase 2 (COX2) and its product, prostaglandin E2 (PGE2), through overexpression of HIF-1α. It has been shown that ligation of PGE2 with its receptor, EP4, robustly promotes cancer progression. HIF-1α/COX2/PGE2/EP4 signaling pathways appear to play an important role in tumor growth. Therefore, we decided to block the expansion of cancer cells by blocking the initiator (HIF-1α) and end (EP4) of this pathway. In this study, we used hyaluronate (HA), and trimethyl chitosan (TMC) recoated superparamagnetic iron oxide nanoparticles (SPIONs) loaded with HIF-1α-silencing siRNA and the EP4 antagonist (E7046) to treat cancer cells and assessed the effect of combination therapy on cancer progression. The results showed that optimum physicochemical characteristics of NPs (size 126.9 nm, zeta potential 27 mV, PDI < 0.2) and linkage of HA with CD44 molecules overexpressed on cancer cells could deliver siRNAs to cancer cells and significantly suppress the HIF-1α in them. Combination therapy of cancer cells by using HIF-1α siRNA-loaded SPION-TMC-HA NPs and E7046 also prevent proliferation, migration, invasion, angiogenesis, and colony formation of the cancer cells, remarkably. © 2020
Related Docs
View other Related Docs
1. Silencing of Hif-1Α/Cd73 Axis by Sirna-Loaded Tat-Chitosan-Spion Nanoparticles Robustly Blocks Cancer Cell Progression, European Journal of Pharmacology (2020)
2. Combined Inhibition of Cd73 and Zeb1 by Arg-Gly-Asp (Rgd)-Targeted Nanoparticles Inhibits Tumor Growth, Colloids and Surfaces B: Biointerfaces (2021)
3. Codelivery of Hif-1Α Sirna and Dinaciclib by Carboxylated Graphene Oxide-Trimethyl Chitosan-Hyaluronate Nanoparticles Significantly Suppresses Cancer Cell Progression, Pharmaceutical Research (2020)
4. Silencing of Il-6 and Stat3 by Sirna Loaded Hyaluronate-N,N,N-Trimethyl Chitosan Nanoparticles Potently Reduces Cancer Cell Progression, International Journal of Biological Macromolecules (2020)
5. Coinhibition of S1pr1 and Gp130 by Sirna-Loaded Alginate-Conjugated Trimethyl Chitosan Nanoparticles Robustly Blocks Development of Cancer Cells, Journal of Cellular Physiology (2020)
6. Concomitant Blockade of A2ar and Ctla-4 by Sirna-Loaded Polyethylene Glycol-Chitosan-Alginate Nanoparticles Synergistically Enhances Antitumor T-Cell Responses, Journal of Cellular Physiology (2020)
7. Inhibition of Cd73 Using Folate Targeted Nanoparticles Carrying Anti-Cd73 Sirna Potentiates Anticancer Efficacy of Dinaciclib, Life Sciences (2020)
8. Blockade of Ctla-4 Increases Anti-Tumor Response Inducing Potential of Dendritic Cell Vaccine, Journal of Controlled Release (2020)
Experts (# of related papers)
View all Related Experts
Fatemeh Atyabi (21)
Jamshid Hadjati (4)
Other Related Docs
9. Codelivery of Stat3 Sirna and Bv6 by Carboxymethyl Dextran Trimethyl Chitosan Nanoparticles Suppresses Cancer Cell Progression, International Journal of Pharmaceutics (2020)
10. Simultaneous Inhibition of Cd73 and Il-6 Molecules by Sirna-Loaded Nanoparticles Prevents the Growth and Spread of Cancer, Nanomedicine: Nanotechnology# Biology# and Medicine (2021)
11. Nanomedicine for Improvement of Dendritic Cell-Based Cancer Immunotherapy, International Immunopharmacology (2020)
12. Silencing Adenosine A2a Receptor Enhances Dendritic Cell-Based Cancer Immunotherapy, Nanomedicine: Nanotechnology# Biology# and Medicine (2020)
13. Combination Cancer Immunotherapy With Dendritic Cell Vaccine and Nanoparticles Loaded With Interleukin-15 and Anti-Beta-Catenin Sirna Significantly Inhibits Cancer Growth and Induces Anti-Tumor Immune Response, Pharmaceutical Research (2022)
14. Silencing of P68 and Stat3 Synergistically Diminishes Cancer Progression, Life Sciences (2020)
15. Hypoxia Inducible Factors in the Tumor Microenvironment As Therapeutic Targets of Cancer Stem Cells, Life Sciences (2019)
16. Blockage of Immune Checkpoint Molecules Increases T-Cell Priming Potential of Dendritic Cell Vaccine, Immunology (2020)
17. Chitosan-Based Smart Stimuli-Responsive Nanoparticles for Gene Delivery and Gene Therapy: Recent Progresses on Cancer Therapy, International Journal of Biological Macromolecules (2024)
18. Increased Susceptibility to Doxorubicin-Induced Cell Death in Acute Lymphocytic Leukemia Cells by Inhibiting Serine/Threonine Wee1 Kinase Expression Using the Chitosan-Carboxymethyl Dextran-Polyethylene Glycol-Tat Nanoparticles, Journal of Drug Delivery Science and Technology (2022)
19. Regulatory T Cells in Breast Cancer As a Potent Anti-Cancer Therapeutic Target, International Immunopharmacology (2020)
20. Development of a T Cell-Targeted Sirna Delivery System Against Hiv-1 Using Modified Superparamagnetic Iron Oxide Nanoparticles: An in Vitro Study, Journal of Pharmaceutical Sciences (2022)
21. Hypoxia Inducible Factor: It’S Role in Angiogenesis and Tumor, Tehran University Medical Journal (2016)
22. 68 Ga-Radiolabeled Bombesin-Conjugated to Trimethyl Chitosan-Coated Superparamagnetic Nanoparticles for Molecular Imaging: Preparation, Characterization and Biological Evaluation, International Journal of Nanomedicine (2019)
23. How Micrornas Affect the Pd-L1 and Its Synthetic Pathway in Cancer, International Immunopharmacology (2020)
24. Downregulation of A2ar by Sirna Loaded Peg-Chitosan-Lactate Nanoparticles Restores the T Cell Mediated Anti-Tumor Responses Through Blockage of Pka/Creb Signaling Pathway, International Journal of Biological Macromolecules (2019)
25. Deferoxamine Preconditioning to Restore Impaired Hif-1Α-Mediated Angiogenic Mechanisms in Adipose-Derived Stem Cells From Stz-Induced Type 1 Diabetic Rats, Cell Proliferation (2015)
26. Carboxymethyl Dextran-Trimethyl Chitosan Coated Superparamagnetic Iron Oxide Nanoparticles: An Effective Sirna Delivery System for Hiv-1 Nef, Journal of Cellular Physiology (2019)
27. Biomedical Applications of Superparamagnetic Nanoparticles in Molecular Scale, Current Organic Chemistry (2015)
28. Unveiling the Theranostic Potential of Spions in Alzheimer's Disease Management, Journal of Psychiatric Research (2024)
29. Theranostic Applications of Stimulus-Responsive Systems Based on Fe2 O3, Pharmaceutical Nanotechnology (2022)
30. Theranostic Α-Lactalbumin-Polymer-Based Nanocomposite As a Drug Delivery Carrier for Cancer Therapy, ACS Biomaterials Science and Engineering (2019)
31. Adenosine Blockage in Tumor Microenvironment and Improvement of Cancer Immunotherapy, Immune Network (2019)
32. Targeting Sirna in Colorectal Cancer Therapy: Nanotechnology Comes Into View, Journal of Cellular Physiology (2019)
33. Nano-Delivery System Targeting to Cancer Stem Cell Cluster of Differentiation Biomarkers, Journal of Controlled Release (2017)
34. Targeted Superparamagnetic Iron Oxide Nanoparticles for Early Detection of Cancer: Possibilities and Challenges, Nanomedicine: Nanotechnology# Biology# and Medicine (2016)
35. Specific Targeting Delivery to Muc1 Overexpressing Tumors by Albumin-Chitosan Nanoparticles Conjugated to Dna Aptamer, International Journal of Pharmaceutics (2016)
36. Application of Nanomedicine for Crossing the Blood–Brain Barrier: Theranostic Opportunities in Multiple Sclerosis, Journal of Immunotoxicology (2016)
37. Signal Amplification Strategy Using Gold/N-Trimethyl Chitosan/Iron Oxide Magnetic Composite Nanoparticles As a Tracer Tag for High-Sensitive Electrochemical Detection, IET Nanobiotechnology (2016)
38. The Antitumor Effect of Topotecan Loaded Thiolated Chitosan-Dextran Nanoparticles for Intravitreal Chemotherapy: A Xenograft Retinoblastoma Model, Journal of Ophthalmic and Vision Research (2023)
39. Anti-Angiogenic Effects of Cd73-Specific Sirna-Loaded Nanoparticles in Breast Cancer-Bearing Mice, Journal of Cellular Physiology (2018)
40. Nanoparticles and Targeted Drug Delivery in Cancer Therapy, Immunology Letters (2017)
41. Optimized Dose of Dendritic Cell-Based Vaccination in Experimental Model of Tumor Using Artificial Neural Network, Iranian Journal of Allergy# Asthma and Immunology (2020)
42. Novel Approach to Improve Vaccine Immunogenicity: Mannosylated Chitosan Nanoparticles Loaded With Recombinant Hepatitis B Antigen As a Targeted Vaccine Delivery System, Journal of Drug Delivery Science and Technology (2018)
43. Targeting Hypoxia and Hypoxia-Inducible Factor-1 in the Tumor Microenvironment for Optimal Cancer Immunotherapy, Journal of Cellular Physiology (2022)
44. Advancing the Therapeutic Effectiveness of Paclitaxel in Chronic Lymphocytic Leukemia Through the Simultaneous Inhibition of Notch1 and Sf3b1, Cancer Cell International (2025)
45. Multiple Sclerosis: Therapeutic Applications of Advancing Drug Delivery Systems, Biomedicine and Pharmacotherapy (2017)
46. Nano-Immunotherapy: Overcoming Delivery Challenge of Immune Checkpoint Therapy, Journal of Nanobiotechnology (2023)
47. Application of Nanoparticle Technology in the Treatment of Systemic Lupus Erythematous, Biomedicine and Pharmacotherapy (2016)