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Combination Cancer Immunotherapy With Dendritic Cell Vaccine and Nanoparticles Loaded With Interleukin-15 and Anti-Beta-Catenin Sirna Significantly Inhibits Cancer Growth and Induces Anti-Tumor Immune Response Publisher Pubmed



Kheshti AMS1 ; Hajizadeh F2 ; Barshidi A1 ; Rashidi B1 ; Ebrahimi F3 ; Bahmanpour S1 ; Karpisheh V1 ; Noukabadi FK4 ; Kiani FK1 ; Hassannia H5 ; Atyabi F6 ; Kiaie SH1, 7 ; Kashanchi F8 ; Navashenaq JG9 Show All Authors
Authors
  1. Kheshti AMS1
  2. Hajizadeh F2
  3. Barshidi A1
  4. Rashidi B1
  5. Ebrahimi F3
  6. Bahmanpour S1
  7. Karpisheh V1
  8. Noukabadi FK4
  9. Kiani FK1
  10. Hassannia H5
  11. Atyabi F6
  12. Kiaie SH1, 7
  13. Kashanchi F8
  14. Navashenaq JG9
  15. Mohammadi H10
  16. Bagherifar R7
  17. Jafari R11, 12
  18. Zolbanin NM12, 13, 14
  19. Jadidiniaragh F1, 15, 16
Show Affiliations
Authors Affiliations
  1. 1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  2. 2. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
  3. 3. Nanoparticle Process Technology, Faculty of Engineering, University of Duisburg-Essen, Duisburg, Germany
  4. 4. Department of Biotechnology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
  5. 5. Immunogenetic Research Center, Faculty of Medicine and Amol Faculty of Paramedical Sciences, Mazandaran University of Medical Sciences, Sari, Iran
  6. 6. Nanotechnology Research Centre, Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, 6715847141, Iran
  8. 8. Laboratory of Molecular Virology, School of Systems Biology, George Mason University, Manassas, VA, United States
  9. 9. Noncommunicable Diseases Research Center, Bam University of Medical Sciences, Bam, Iran
  10. 10. Non-communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, Iran
  11. 11. Nephrology and Kidney Transplant Research Center, Clinical Research Institute, Urmia University of Medical Sciences, Urmia, Iran
  12. 12. Hematology, Immune Cell Therapy, and Stem Cell Transplantation Research Center, Clinical Research Institute, Urmia University of Medical Sciences, Urmia, Iran
  13. 13. Experimental and Applied Pharmaceutical Research Center, Urmia University of Medical Sciences, Urmia, Iran
  14. 14. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran
  15. 15. Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  16. 16. Research Center for Integrative Medicine in Aging, Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran

Source: Pharmaceutical Research Published:2022


Abstract

Purpose: The invention and application of new immunotherapeutic methods can compensate for the inefficiency of conventional cancer treatment approaches, partly due to the inhibitory microenvironment of the tumor. In this study, we tried to inhibit the growth of cancer cells and induce anti-tumor immune responses by silencing the expression of the β-catenin in the tumor microenvironment and transmitting interleukin (IL)-15 cytokine to provide optimal conditions for the dendritic cell (DC) vaccine. Methods: For this purpose, we used folic acid (FA)-conjugated SPION-carboxymethyl dextran (CMD) chitosan (C) nanoparticles (NPs) to deliver anti-β-catenin siRNA and IL-15 to cancer cells. Results: The results showed that the codelivery of β-catenin siRNA and IL-15 significantly reduced the growth of cancer cells and increased the immune response. The treatment also considerably stimulated the performance of the DC vaccine in triggering anti-tumor immunity, which inhibited tumor development and increased survival in mice in two different cancer models. Conclusions: These findings suggest that the use of new nanocarriers such as SPION-C-CMD-FA could be an effective way to use as a novel combination therapy consisting of β-catenin siRNA, IL-15, and DC vaccine to treat cancer. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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