Detection of Glioblastoma Multiforme Using Quantitative Molecular Magnetic Resonance Imaging Based on 5-Aminolevulinic Acid: In Vitro and in Vivo Studies Publisher Pubmed



Ebrahimpour A¹ ; Tirgar F² ; Hajipourverdom B³ ; Abbasi A⁴ ; Hadjighassem M⁵ ; Abdolmaleki P³ ; Hosseindoost S⁵ ; Javadi SAH⁶ ; Hashemi H⁷ ; Foroushani AR⁸ ; Alam NR^{1, 9} ; Khoobi M^{10, 11}
Source: Magnetic Resonance Materials in Physics# Biology and Medicine Published:2022

Abstract

Objectives: We demonstrated a novel metabolic method based on sequential administration of 5-aminolevulinic acid (ALA) and iron supplement, and ferric ammonium citrate (FAC), for glioblastoma multiforme (GBM) detection using R2’ and quantitative susceptibility mapping (QSM). Materials and methods: Intra-cellular iron accumulation in glioblastoma cells treated with ALA and/or FAC was measured. Cell phantoms containing glioblastoma cells and Wistar rats bearing C6 glioblastoma were imaged using a 3 T MRI scanner after sequential administration of ALA and FAC. The relaxivity and QSM analysis were performed on the images. Results: The intra-cellular iron deposition was significantly higher in the glioma cells with sequential treatment of ALA and FAC for 6 h compared to those treated with the controls. The relaxivity and magnetic susceptibility values of the glioblastoma cells and rat brain tumors treated with ALA + FAC (115 ± 5 s-1 for R2’, and 0.1 ± 0.02 ppm for magnetic susceptibility) were significantly higher than those treated with the controls (55 ± 18 (FAC), 45 ± 15 (ALA) s-1 for R2’, p < 0.05, and 0.03 ± 0.03 (FAC), 0.02 ± 0.02 (ALA) ppm for magnetic susceptibility, p < 0.05). Discussion: Sequential administration of ALA and iron supplements increases the iron deposition in glioblastoma cells, enabling clinical 3 T MRI to detect GBM using R2’ or QSM. © 2021, The Author(s), under exclusive licence to European Society for Magnetic Resonance in Medicine and Biology (ESMRMB).