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Molecular Identification of Rhipicephalus Species (Acari: Ixodidae) Parasitizing Livestock From Iran Publisher Pubmed



Hosseinichegeni A1, 2 ; Nasrabadi M3 ; Sadat Hashemiaghdam S3 ; Oshaghi MA3 ; Lotfi A4 ; Telmadarraiy Z3 ; Sedaghat MM3
Authors
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Authors Affiliations
  1. 1. Department of Plant Protection Faculty of Agriculture, University of Lorestan, Khorramabad, Iran
  2. 2. Razi Herbal Medicines Research Center Lorestan University of Medical Sciences, Khorramabad, Iran
  3. 3. Department of Medical Entomology and Vector Control School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Damavand Agricultural College Technical and Vocational University, Tehran, Iran

Source: Mitochondrial DNA Part A: DNA Mapping# Sequencing# and Analysis Published:2019


Abstract

This study was designed to identify populations of Rhipicephalus sanguineus collected from Iran and also to study molecular taxonomy of Rhipicephalus species using cytochrome C oxidase subunit 1 (COI) and internal transcribed spacer 2 (ITS2) sequences. Tick specimens were collected from livestock (sheep and goat) in 14 Iranian provinces. DNA of individual specimens was extracted and PCR was done on these samples. So, 62 sequencing (33 COI and 29 ITS2) were done, successfully. Morphologically, we identified four Rhipicephalus species, namely R. bursa, R. sanguineus (s.l.), R. sanguineus (s.s.), and R. turanicus based on taxonomic keys. The data obtained from the phylogenetic analyses of COI and ITS2 fragments present a possible conflict regarding the identity of R. sanguineus species. Thus, the molecular identification of R. sanguineus group might be different according to mitochondrial and nuclear DNA. The results show a phylogenic conflict based on COI and ITS2 phylogeny in a tree topology. We dealt with three genetic entities in R. sanguineus group (i.e. R. sanguineus (s.s.), R. sanguineus (s.l.), and R. turanicus) based on COI phylogeny and two genetic clades (i.e. R. sanguineus (s.s.) and R. sanguineus (s.l.)/R. turanicus) according to ITS2 phylogeny. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
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