Display of B. Pumilus Chitinase on the Surface of B. Subtilis Spore As a Potential Biopesticide

Display of B. Pumilus Chitinase on the Surface of B. Subtilis Spore As a Potential Biopesticide Publisher Pubmed

Rostami A^{1, 3} ; Hinc K² ; Goshadrou F³ ; Shali A¹ ; Bayat M¹ ; Hassanzadeh M⁴ ; Amanlou M⁴ ; Eslahi N¹ ; Ahmadian G¹

Show Affiliations

1. Department of Industrial and Environmental Biotechnology, National Institute of Genetics Engineering and Biotechnology (NIGEB), Tehran, Iran
2. Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology, UG-MUG, Gdansk, Poland
3. Department of Physiology, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4. Department of Medicinal Chemistry, Drug Design and Development Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: Pesticide Biochemistry and Physiology Published:2017

Abstract

Background Chitinases can inhibit the growth of many fungal diseases which are a great threat for global agricultural production. Biological control of pathogens like fungi, is believed to be one of the best ways to eliminate the adverse effects of plant pathogens. To this end, we expressed and displayed a chitinase from Bacillus pumilus (ChiS) on the surface of Bacillus subtilis spores, as a biocontrol agent. Result ChiS enzyme from B. pumilus was expressed on the spores of B. subtilis using CotG as a carrier protein. Immunofluorescence microscopy confirmed the expression of ChiS on the surface of the spores. Enzyme activity assay showed that the surface displayed ChiS was active and was also able to inhibit the growth of Rhizoctonia solani and Trichoderma harzianum fungi. Western blot analysis also indicated that CotG-ChiS is partially processed after display. Molecular dynamics simulation showed that the stability of the heterologous protein was decreased after fusion. Conclusion ChiS was successfully displayed on the surface of Bacillus spores by fusion to the CotG, one of the main spore coat proteins. In-vitro experiments showed that the displayed enzyme was effective in growth inhibition of R. solani and T. harzianum fungi. © 2017 Elsevier B.V.

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Style	Citing Format
MLA	Rostami A, et al.. "Display of B. Pumilus Chitinase on the Surface of B. Subtilis Spore As a Potential Biopesticide." Pesticide Biochemistry and Physiology, vol. 140, no. , 2017, pp. 17-23.
APA	Rostami A, Hinc K, Goshadrou F, Shali A, Bayat M, Hassanzadeh M, Amanlou M, Eslahi N, Ahmadian G (2017). Display of B. Pumilus Chitinase on the Surface of B. Subtilis Spore As a Potential Biopesticide. Pesticide Biochemistry and Physiology, 140(), 17-23.
Chicago	Rostami A, Hinc K, Goshadrou F, Shali A, Bayat M, Hassanzadeh M, Amanlou M, Eslahi N, Ahmadian G. "Display of B. Pumilus Chitinase on the Surface of B. Subtilis Spore As a Potential Biopesticide." Pesticide Biochemistry and Physiology 140, no. (2017): 17-23.
Harvard	Rostami A et al. (2017) 'Display of B. Pumilus Chitinase on the Surface of B. Subtilis Spore As a Potential Biopesticide', Pesticide Biochemistry and Physiology, 140(), pp. 17-23.
Vancouver	Rostami A, Hinc K, Goshadrou F, Shali A, Bayat M, Hassanzadeh M, et al.. Display of B. Pumilus Chitinase on the Surface of B. Subtilis Spore As a Potential Biopesticide. Pesticide Biochemistry and Physiology. 2017;140():17-23.
BibTex	@article{ author = {Rostami A and Hinc K and Goshadrou F and Shali A and Bayat M and Hassanzadeh M and Amanlou M and Eslahi N and Ahmadian G}, title = {Display of B. Pumilus Chitinase on the Surface of B. Subtilis Spore As a Potential Biopesticide}, journal = {Pesticide Biochemistry and Physiology}, volume = {140}, number = {}, pages = {17-23}, year = {2017} }
RIS	TY - JOUR AU - Rostami A AU - Hinc K AU - Goshadrou F AU - Shali A AU - Bayat M AU - Hassanzadeh M AU - Amanlou M AU - Eslahi N AU - Ahmadian G TI - Display of B. Pumilus Chitinase on the Surface of B. Subtilis Spore As a Potential Biopesticide JO - Pesticide Biochemistry and Physiology VL - 140 IS - SP - 17 EP - 23 PY - 2017 ER -

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