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Molecular Typing of Clostridioides Difficile Isolates From Clinical and Non-Clinical Samples in Iran Publisher Pubmed



Kouhsari E1, 2 ; Douraghi M3 ; Fakhre Yaseri H4 ; Talebi M1 ; Ahmadi A2 ; Sholeh M1 ; Amirmozafari N1
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran
  3. 3. Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Research Center for Gastroenterology and Liver Disease, Firouzgar Hospital, Iran University of Medical Sciences, Tehran, Iran

Source: APMIS Published:2019


Abstract

Clostridioides difficile is a major cause of nosocomial infectious diarrhea in hospitalized patients throughout the world. We aimed to characterize C. difficile isolates among hospitalized patients, hospital staffs, and hospital environment samples obtained in three tertiary care hospitals of Iran with regard to their molecular types between June 2016 and November 2017. The toxigenicity of C. difficile isolates was determined by toxigenic culture and multiplex-PCR. Toxigenic C. difficile isolates collected were ribotyped using capillary gel electrophoresis-based PCR and the database of WEBRIBO (http://webribo.ages.at). Of 500 clinical and non-clinical samples, toxigenic C. difficile were identified in 35 of 250 stool samples (14%) and in 3 of 250 swabs (1.2%). The most frequently found ribotypes (RTs) were 039, AI-12, and AI-21 (15.8, 10.52, and 10.52% of all isolates, respectively). Further RTs were: 017, 001, AI-3, AI-15, AI-18, AI-10, AI-4, and PR21195 (as new ribotype). The epidemic RTs (027 and 078) seen in the Europe, North America, and Asia were completely absent in this study. © 2019 APMIS. Published by John Wiley & Sons Ltd
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