Inclusion of Ovine Enriched Serum With Vitamin E and Polyunsaturated Fatty Acids in the Freezing Medium: A New Strategy to Improve Human Frozen-Thawed Sperm Parameters

Inclusion of Ovine Enriched Serum With Vitamin E and Polyunsaturated Fatty Acids in the Freezing Medium: A New Strategy to Improve Human Frozen-Thawed Sperm Parameters Publisher Pubmed

Seifi S¹ ; Shahverdi A^{1, 2} ; Rezaei Topraggaleh T^{1, 3} ; Sharafi M^{1, 4} ; Esmaeili V¹ ; Choobineh H⁵ ; Zamiri MJ⁶ ; Habibi M⁷ ; Alizadeh A¹

Show Affiliations

1. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
2. Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
3. Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
4. Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
5. School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
6. Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
7. Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK, United States

Source: Andrologia Published:2020

Abstract

The objective was to evaluate the effect of inclusion of 2.5% and 5% ovine serum, enriched with vitamin E (Vit E) and fish oil (FO), in human sperm freezing medium. Serum samples were prepared from sixteen rams (n = 4) feeding on a without supplemented diet, and diets supplemented with Vit E, FO and Vit E + FO. Semen samples, from 60 normozoospermic men, were frozen in: (I) a commercial freezing medium (SpermFreeze™; control medium), (II) the commercial freezing medium containing foetal bovine serum, (III) the commercial freezing medium + nonenriched serum (serum group), (IV) the commercial freezing medium + Vit E enriched serum (Vit E group), (V) the commercial freezing medium + FO enriched serum (FO group) and (VI) the commercial freezing medium + Vit E + FO enriched serum (Vit E + FO group). Sperm total and progressive motility, morphology, viability and plasma membrane integrity were significantly higher (p ≤.05) in Vit E and Vit E + FO groups compared with the control group. Mitochondrial membrane potential did not differ between treatments (p >.05). It was concluded that ovine serum enriched with vitamin E and vitamin E + FO improved the quality of human spermatozoa but enriched serum containing FO could not improve the sperm cryo-injuries. © 2020 Blackwell Verlag GmbH

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Style	Citing Format
MLA	Seifi S, et al.. "Inclusion of Ovine Enriched Serum With Vitamin E and Polyunsaturated Fatty Acids in the Freezing Medium: A New Strategy to Improve Human Frozen-Thawed Sperm Parameters." Andrologia, vol. 52, no. 4, 2020, pp. -.
APA	Seifi S, Shahverdi A, Rezaei Topraggaleh T, Sharafi M, Esmaeili V, Choobineh H, Zamiri MJ, Habibi M, Alizadeh A (2020). Inclusion of Ovine Enriched Serum With Vitamin E and Polyunsaturated Fatty Acids in the Freezing Medium: A New Strategy to Improve Human Frozen-Thawed Sperm Parameters. Andrologia, 52(4), -.
Chicago	Seifi S, Shahverdi A, Rezaei Topraggaleh T, Sharafi M, Esmaeili V, Choobineh H, Zamiri MJ, Habibi M, Alizadeh A. "Inclusion of Ovine Enriched Serum With Vitamin E and Polyunsaturated Fatty Acids in the Freezing Medium: A New Strategy to Improve Human Frozen-Thawed Sperm Parameters." Andrologia 52, no. 4 (2020): -.
Harvard	Seifi S et al. (2020) 'Inclusion of Ovine Enriched Serum With Vitamin E and Polyunsaturated Fatty Acids in the Freezing Medium: A New Strategy to Improve Human Frozen-Thawed Sperm Parameters', Andrologia, 52(4), pp. -.
Vancouver	Seifi S, Shahverdi A, Rezaei Topraggaleh T, Sharafi M, Esmaeili V, Choobineh H, et al.. Inclusion of Ovine Enriched Serum With Vitamin E and Polyunsaturated Fatty Acids in the Freezing Medium: A New Strategy to Improve Human Frozen-Thawed Sperm Parameters. Andrologia. 2020;52(4):-.
BibTex	@article{ author = {Seifi S and Shahverdi A and Rezaei Topraggaleh T and Sharafi M and Esmaeili V and Choobineh H and Zamiri MJ and Habibi M and Alizadeh A}, title = {Inclusion of Ovine Enriched Serum With Vitamin E and Polyunsaturated Fatty Acids in the Freezing Medium: A New Strategy to Improve Human Frozen-Thawed Sperm Parameters}, journal = {Andrologia}, volume = {52}, number = {4}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Seifi S AU - Shahverdi A AU - Rezaei Topraggaleh T AU - Sharafi M AU - Esmaeili V AU - Choobineh H AU - Zamiri MJ AU - Habibi M AU - Alizadeh A TI - Inclusion of Ovine Enriched Serum With Vitamin E and Polyunsaturated Fatty Acids in the Freezing Medium: A New Strategy to Improve Human Frozen-Thawed Sperm Parameters JO - Andrologia VL - 52 IS - 4 SP - EP - PY - 2020 ER -

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