Tehran University of Medical Sciences

Science Communicator Platform

Share By
Amniotic Membrane Mesenchymal Stem Cells Can Differentiate Into Germ Cells in Vitro Publisher Pubmed



Afsartala Z1 ; Rezvanfar MA2 ; Hodjat M3 ; Tanha S3 ; Assadollahi V4 ; Bijangi K5 ; Abdollahi M2 ; Ghasemzadehhasankolaei M6
Authors

Source: In Vitro Cellular and Developmental Biology - Animal Published:2016


Abstract

This is the first report on differentiation of mouse amniotic membrane mesenchymal stem cells (AM-MSCs) into male germ cells (GCs). AM-MSCs have the multipotent differentiation capacity and can be differentiated into various cell types. In the present study, AM-MSCs were induced for differentiation into GCs. AM-MSCs were isolated from mouse embryonic membrane by enzymatic digestion. AM-MSCs were characterized with osteogenic and adipogenic differentiation test and flow cytometric analysis of some CD-markers. AM-MSCs were induced to differentiate into GCs using a creative two-step method. Passage-3 AM-MSCs were firstly treated with 25 ng/ml bone morphogenetic protein 4 (BMP4) for 5 d and in continuing with 1 μM retinoic acid (RA) for 12 d (total treatment time was 17 d). At the end of the treatment period, real-time reverse transcription (RT)-PCR was performed to evaluate the expression of GC-specific markers—Itgb1, Dazl, Stra8, Piwil2, Mvh, Oct4, and c-Kit- in the cells. Moreover, flow cytometry and immunofluorescence staining were performed to evaluate the expression of Mvh and Dazl at protein level. Real-time RT-PCR showed that most of the tested markers were upregulated in the treated AM-MSCs. Furthermore, flow cytometric and immunofluorescence analyses both revealed that a considerable part of the treated cells expressed GC-specific markers. The percentage of positive cells for Mvh and Dazl was about 23 and 46%, respectively. Our results indicated that a number of AM-MSCs successfully differentiated into the GCs. Finally, it seems that AM-MSCs would be a potential source of adult pluripotent stem cells for in vitro generation of GCs and cell-based therapies for treatment of infertility. © 2016, The Society for In Vitro Biology.
Related Docs
View other Related Docs
1. Mesenchymal Stromal Cells; a New Horizon in Regenerative Medicine, Journal of Cellular Physiology (2020)
2. Comparison of the Efficacy of Bone Morphogenetic Protein-4 on in Vitro Differentiation of Murine Adipose and Bone Marrow Mesenchymal Stem Cells Into Primordial Germ Cells, Research in Pharmaceutical Sciences (2022)
3. A Comparative Study of the Bone Marrow and Umbilical Cord-Derived Mesenchymal Stem Cells (Mscs) Efficiency on Generating Msc-Educated Macrophages (Mems), Asian Pacific Journal of Cancer Prevention (2022)
Experts (# of related papers)
View all Related Experts
Ashraf Moini (2)
Fardin Amidi (1)
Other Related Docs
4. Optimizing the Seeding Density of Human Mononuclear Cells to Improve the Purity of Highly Proliferative Mesenchymal Stem Cells, Bioengineering (2023)
5. Dynamic Changes of Epigenetic Signatures During Chondrogenic and Adipogenic Differentiation of Mesenchymal Stem Cells, Biomedicine and Pharmacotherapy (2017)
6. Induction of Mouse Peritoneum Mesenchymal Stem Cells Into Germ Cell-Like Cells Using Follicular Fluid and Cumulus Cells-Conditioned Media, Stem Cells and Development (2019)
7. A Comparative Study to Evaluate Myogenic Differentiation Potential of Human Chorion Versus Umbilical Cord Blood-Derived Mesenchymal Stem Cells, Tissue and Cell (2017)
8. Challenges in Mesenchymal Stromal Cell-Based Therapies, Current Stem Cell Research and Therapy (2023)
9. Effect of Secreted Molecules of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells on Acute Hepatic Failure Model, Stem Cells and Development (2016)
10. The Effect of Mesenchymal Stem Cell-Derived Microvesicles on Differentiation of Umbilical Cord Blood-Derived Cd34+ Cells Toward Myeloid Lineage, Gene Reports (2022)
11. Wharton's Jelly Derived Mesenchymal Stem Cells Differentiate Into Oocyte Like Cells in Vitro by Follicular Fluid and Cumulus Cells Conditioned Medium, Heliyon (2020)
12. Mesenchymal Stem/Stromal Cells As a Valuable Source for the Treatment of Immune-Mediated Disorders, Stem Cell Research and Therapy (2021)
13. Rapid Induction of Neural Differentiation in Human Umbilical Cord Matrix Mesenchymal Stem Cells by Camp-Elevating Agents, International Journal of Molecular and Cellular Medicine (2016)
14. Crispr/Cas9-Engineered Mesenchymal Stromal/Stem Cells and Their Extracellular Vesicles: A New Approach to Overcoming Cell Therapy Limitations, Biomedicine and Pharmacotherapy (2022)
15. Signaling Crosstalk Between Mesenchymal Stem Cells and Tumor Cells: Implications for Tumor Suppression or Progression, Cytokine and Growth Factor Reviews (2024)
16. The Restorative Effect of Human Amniotic Fluid Stem Cells on Spinal Cord Injury, Cells (2021)
17. Transduction of Mesenchymal Stem Cells That Express Vegf-A and Pre-Conditioning With Sdf1α in Order to Increase Survival for Use in Myocardial Infarction Treatment in a Rat Model, Modares Journal of Medical Sciences: Pathobiology (2015)
18. The Beneficial Effects of Mesenchymal Stem Cells and Their Exosomes on Myocardial Infarction and Critical Considerations for Enhancing Their Efficacy, Ageing Research Reviews (2023)
19. Biological Characteristics and Optical Reflectance Spectroscopy of Human Placenta Derived Mesenchymal Stem Cells for Application in Regenerative Medicine, Journal of Lasers in Medical Sciences (2021)
20. Bones Morphogenic Protein-4 and Retinoic Acid Combined Treatment Comparative Analysis for in Vitro Differentiation Potential of Murine Mesenchymal Stem Cells Derived From Bone Marrow and Adipose Tissue Into Germ Cells, Microscopy Research and Technique (2017)