Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Male Germ-Like Cell Differentiation Potential of Human Umbilical Cord Wharton’S Jelly-Derived Mesenchymal Stem Cells in Co-Culture With Human Placenta Cells in Presence of Bmp4 and Retinoic Acid



Ataie Nejad N1 ; Amidi F2 ; Agha Hoseini M3 ; Nayer Nia K4 ; Habibi M5 ; Kajbafzadeh AM6 ; Mazaheri Z7 ; Yamini N1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Reproductive Biology, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Anatomical Sciences, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. IVF Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Institute for Molecular Medicine and Cell Therapy, Dusseldorf, Germany and GENEOCELL, Advanced Molecular and Cellular Technologies, Tehran, Iran
  5. 5. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
  6. 6. Pediatric Urology Research Center, Children Hospital Medical Center, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Anatomical Sciences, Tarbiat Modares University, Tehran, Iran

Source: Iranian Journal of Basic Medical Sciences Published:2015

Abstract

Objective(s): Mesenchymal stem cells (MSCs) derived from Wharton’s jelly (WJ-MSCs) are now much more appealing for cell-based infertility therapy. Hence, WJ-MSCs differentiation toward germ layer cells for cell therapy purposes is currently under intensive study. Materials and Methods: MSCs were isolated from human Wharton’s jelly and treated with BMP4, retinoic acid (RA) or co-cultured on human amniotic epithelial (HAE) and chorionic plate (HCP) placenta feeder cells. profile of POU5F1, Fragilis, Plzf, DDX4, Piwil2, Stra8, Dazl, β1- and α6-integrins (ITΒ1, ITA6) genes expression as germ cell markers were analyzed using RT-PCR and real-time PCR. Immunocytochemistry of surface markers were conducted. Results: After 3 weeks treatment with different reagents and co-culture system, morphology of WJ-MSCs changed to shiny clusters and germ cell specific markers in mRNA were up-regulated in both placental feeder + RA and BMP4 + RA. Induction of hWJ-MSCs with BMP4 in presence of RA resulted in significant up-regulation (P≤0.05) of all germ cell specific genes (c-Kit; 2.84±0.59, DDX4; 1.69±0.39, Piwil2; 1.14±0.21, Dazl; 0.65±0.25, α6 integrin; 1.26±0.53, β1 integrins; 1.18±0.65) compared to control and placental feeder cells + RA. Our results indicated that HAE and HCP followed by RA treatment were involved in human germ cell development. Conclusion: We demonstrated that under the right conditions, hWJ-MSCs have the ability to differentiate to germ cells and this provides an excellent pattern to study infertility cause and treatment. © 2015, Mashhad University of Medical Sciences. All rights reserved.
Related Docs
View other Related Docs
1. In Vitro Differentiation Process of Human Wharton’S Jelly Mesenchymal Stem Cells to Male Germ Cells in the Presence of Gonadal and Non-Gonadal Conditioned Media With Retinoic Acid, In Vitro Cellular and Developmental Biology - Animal (2015)
2. Comparison of Human Amniotic, Chorionic, and Umbilical Cord Multipotent Mesenchymal Stem Cells Regarding Their Capacity for Differentiation Toward Female Germ Cells, Cellular Reprogramming (2017)
3. Germline Cells Derived From Mesenchymal Stem Cells, With the Focus on Wharton's Jelly, Asian Pacific Journal of Reproduction (2018)
4. Amniotic Membrane Mesenchymal Stem Cells Can Differentiate Into Germ Cells in Vitro, In Vitro Cellular and Developmental Biology - Animal (2016)
5. Possibilities in Germ Cell Research: An Engineering Insight, Trends in Biotechnology (2015)
6. Sertoli Cell Condition Medium Can Induce Germ Like Cells From Bone Marrow Derived Mesenchymal Stem Cells, Iranian Journal of Basic Medical Sciences (2016)
7. Human Wharton’S Jelly-Derived Mesenchymal Stem Cells Express Oocyte Developmental Genes During Co-Culture With Placental Cells, Iranian Journal of Basic Medical Sciences (2015)
8. Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea], International Journal of Morphology (2017)
Experts (# of related papers)
View all Related Experts
Tayebeh Rastegar (3)
Iraj Ragerdi Kashani (3)
Other Related Docs
9. Embryonic Stem Cells and Infertility, American Journal of Perinatology (2018)
10. Bone Tissue Engineering Using Human Cells: A Comprehensive Review on Recent Trends, Current Prospects, and Recommendations, Applied Sciences (Switzerland) (2019)
11. Derivation of Male Germ Cells From Ram Bone Marrow Mesenchymal Stem Cells by Three Different Methods and Evaluation of Their Fate After Transplantation Into the Testis, In Vitro Cellular and Developmental Biology - Animal (2016)
12. Differentiation of Bone Marrow-Derived Stage-Specific Embryonic Antigen 1 Positive Pluripotent Stem Cells Into Male Germ Cells, Microscopy Research and Technique (2017)
13. Comparison of the Efficacy of Bone Morphogenetic Protein-4 on in Vitro Differentiation of Murine Adipose and Bone Marrow Mesenchymal Stem Cells Into Primordial Germ Cells, Research in Pharmaceutical Sciences (2022)
14. Retinoic Acid As the Stimulating Factor for Differentiation of Wharton’S Jelly– Mesenchymal Stem Cells Into Hepatocyte-Like Cells, Avicenna Journal of Medical Biotechnology (2015)
15. Differentiation of Wharton’S Jelly-Derived Mesenchymal Stem Cells Into Motor Neuron-Like Cells on Three-Dimensional Collagen-Grafted Nanofibers, Molecular Neurobiology (2016)
16. Application of Stem Cells to Infertility Treatment With Emphasis on Mesenchymal Stem Cells and Ovarian Stem Cells, American Journal of Perinatology (2018)
17. Differentiation of Human Endometrial Stem Cells Encapsulated in Alginate Hydrogel Into Oocyte-Like Cells, BioImpacts (2023)
18. Application of Mesenchymal Stem Cells (Mscs) in Neurodegenerative Disorders: History, Findings, and Prospective Challenges, Pathology Research and Practice (2023)
19. Bones Morphogenic Protein-4 and Retinoic Acid Combined Treatment Comparative Analysis for in Vitro Differentiation Potential of Murine Mesenchymal Stem Cells Derived From Bone Marrow and Adipose Tissue Into Germ Cells, Microscopy Research and Technique (2017)
20. Sertoli Cell–Conditioned Medium Induces Differentiation of Bone Marrow–Derived Mesenchymal Stem Cells to Male Germ-Like Cells in Busulfan-Induced Azoospermic Mouse Model, Reproductive Sciences (2024)
21. Rapid Induction of Neural Differentiation in Human Umbilical Cord Matrix Mesenchymal Stem Cells by Camp-Elevating Agents, International Journal of Molecular and Cellular Medicine (2016)
22. Primordial Germ Cell Differentiation of Nuclear Transfer Embryonic Stem Cells Using Surface Modified Electroconductive Scaffolds, In Vitro Cellular and Developmental Biology - Animal (2017)
23. Expression Analysis of Tsga10 During in Vitro Differentiation of Germ Cells From Mouse Embryonic Stem Cell, Tehran University Medical Journal (2015)
24. Multipotent Stem Cell and Current Application, Acta Medica Iranica (2017)