Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Enhanced Sciatic Nerve Regeneration by Human Endometrial Stem Cells in an Electrospun Poly (Ε-Caprolactone)/Collagen/Nbg Nerve Conduit in Rat Publisher Pubmed



Mohamadi F1 ; Ebrahimibarough S1 ; Nourani MR2 ; Mansoori K3 ; Salehi M1 ; Alizadeh AA4 ; Tavangar SM5 ; Sefat F6 ; Sharifi S7 ; Ai J1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Nano Biotechnology Research Centre, Baqiyatallah University of Medical Sciences, Tehran, Iran
  3. 3. Neuromusculoskletal Research Centre Firozgar Hospital, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Tissue Engineering and Applied Cell Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
  5. 5. Department of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Medical Engineering, School of Engineering, University of Bradford, Bradford, United Kingdom
  7. 7. Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

Source: Artificial Cells# Nanomedicine and Biotechnology Published:2018


Abstract

In recent years, for neurodegenerative diseases therapy, research has focused on the stem cells therapy. Due to promising findings in stem cell therapy, there are various sources of stem cells for transplantation in human. The aim of this study was to evaluate sciatic nerve regeneration in the rat after nerve transaction followed by human endometrial stem cells (hEnSCs) treatment into poly (e-caprolactone)/collagen/nanobioglass (PCL/collagen/NBG) nanofibrous conduits. After treatment of animals, the performance in motor and sensory tests, showed significant improvement in rats treated with hEnSCs as an autograft. H&E images provided from cross-sectional and, longitudinal-sections of the harvested regenerative nerve as well as immunohistochemistry results indicated that regenerative nerve fibres had been formed and accompanied with new blood vessels in the conduit cell group. Due to the advantage of high surface area for cell attachment, it is reported that this electrospun nerve conduit could find more application in cell therapy for nerve regeneration in future, to further improve the functional regeneration outcome, especially for longer nerve defect restoration. In conclusion, our results suggest that the PCL/collagen/NBG nanofibrous conduit filled with hEnSCs is a suitable strategy to improve nerve regeneration after a nerve transaction in rat. © 2017, © 2017 Informa UK Limited, trading as Taylor & Francis Group.
Related Docs
View other Related Docs
1. Use New Poly (Ε-Caprolactone/Collagen/Nbg) Nerve Conduits Along With Ngf for Promoting Peripheral (Sciatic) Nerve Regeneration in a Rat, Artificial Cells# Nanomedicine and Biotechnology (2018)
2. Electrospun Nerve Guide Scaffold of Poly(Ε-Caprolactone)/Collagen/Nanobioglass: An in Vitro Study in Peripheral Nerve Tissue Engineering, Journal of Biomedical Materials Research - Part A (2017)
3. Bridging Potential of Taurine-Loading Pcl Conduits Transplanted With Henscs on Resected Sciatic Nerves, Regenerative Therapy (2022)
4. Enhanced Sciatic Nerve Regeneration With Fibrin Scaffold Containing Human Endometrial Stem Cells and Insulin Encapsulated Chitosan Particles: An in Vivo Study, Injury (2023)
5. Preparation of Bilayer Tissue-Engineered Polyurethane/Poly-L-Lactic Acid Nerve Conduits and Their in Vitro Characterization for Use in Peripheral Nerve Regeneration, Journal of Biological Engineering (2024)
6. Mesenchymal Endometrial Stem/Stromal Cells for Hard Tissue Engineering: A Review of in Vitro and in Vivo Evidence, Regenerative Medicine (2017)
7. Enhanced Sciatic Nerve Regeneration by Poly-L-Lactic Acid/Multi-Wall Carbon Nanotube Neural Guidance Conduit Containing Schwann Cells and Curcumin Encapsulated Chitosan Nanoparticles in Rat, Materials Science and Engineering C (2020)
8. Prospects of Peripheral Nerve Tissue Engineering Using Nerve Guide Conduits Based on Silk Fibroin Protein and Other Biopolymers, International Materials Reviews (2017)
Experts (# of related papers)
View all Related Experts
Somayeh Ebrahimi Barough (24)
Mahmoud Azami (6)
Other Related Docs
9. Evaluation of Motor Neuron-Like Cell Differentiation of Henscs on Biodegradable Plga Nanofiber Scaffolds, Molecular Neurobiology (2015)
10. Endometrial and Menstrual Blood Mesenchymal Stem/Stromal Cells: Biological Properties and Clinical Application, Frontiers in Cell and Developmental Biology (2020)
11. Bioactive Glasses: Sprouting Angiogenesis in Tissue Engineering, Trends in Biotechnology (2018)
12. Pcl/Gelatin Nanofibrous Scaffolds With Human Endometrial Stem Cells/Schwann Cells Facilitate Axon Regeneration in Spinal Cord Injury, Journal of Cellular Physiology (2019)
13. Effectiveness of Reinforced 45S5 Bioglass With Yttria-Stabilized Tetragonal Zirconia and Iron in the Regeneration of Rabbit Calvarial Defects, Acta Veterinaria Eurasia (2022)
14. Purmorphamine As a Shh Signaling Activator Small Molecule Promotes Motor Neuron Differentiation of Mesenchymal Stem Cells Cultured on Nanofibrous Pcl Scaffold, Molecular Neurobiology (2017)
15. Polyurethane/Gelatin Nanofibrils Neural Guidance Conduit Containing Platelet-Rich Plasma and Melatonin for Transplantation of Schwann Cells, Cellular and Molecular Neurobiology (2018)
16. Stem Cell Therapy in Limb Ischemia: State-Of-Art, Perspective, and Possible Impacts of Endometrial-Derived Stem Cells, Frontiers in Cell and Developmental Biology (2022)
17. Inhibitor of Pi3k/Akt Signaling Pathway Small Molecule Promotes Motor Neuron Differentiation of Human Endometrial Stem Cells Cultured on Electrospun Biocomposite Polycaprolactone/Collagen Scaffolds, Molecular Neurobiology (2017)
18. Differentiation of Human Endometrial Stem Cells Into Schwann Cells in Fibrin Hydrogel As 3D Culture, Molecular Neurobiology (2016)
19. Defining the Role of 17Β-Estradiol in Human Endometrial Stem Cells Differentiation Into Neuron-Like Cells, Cell Biology International (2021)
20. Fabrication and Characterization of Nanofibrous Poly (L-Lactic Acid)/Chitosan-Based Scaffold by Liquid–Liquid Phase Separation Technique for Nerve Tissue Engineering, Molecular Biotechnology (2021)
21. Transplantation of Mir-219 Overexpressed Human Endometrial Stem Cells Encapsulated in Fibrin Hydrogel in Spinal Cord Injury, Journal of Cellular Physiology (2019)
22. Improvement of Sciatic Nerve Regeneration by Multichannel Nanofibrous Membrane-Embedded Electro-Conductive Conduits Functionalized With Laminin, Journal of Materials Science: Materials in Medicine (2022)
23. Bioactive Glasses and Calcium Phosphates, Biomaterials for Oral and Dental Tissue Engineering (2017)
24. Mouse Preantral Follicle Growth in 3D Co-Culture System Using Human Menstrual Blood Mesenchymal Stem Cell, Reproductive Biology (2018)
25. Investigating the Neuroglial Differentiation Effect of Neuroblastoma Conditioned Medium in Human Endometrial Stem Cells Cultured on 3D Nanofibrous Scaffold, Journal of Biomedical Materials Research - Part A (2015)
26. Augmenting Peripheral Nerve Regeneration Using Hair Follicle Stem Cells in Rats, Basic and Clinical Neuroscience (2022)
27. The Effect of Purmorphamine on Differentiation of Endometrial Stem Cells Into Osteoblast-Like Cells on Collagen/Hydroxyapatite Scaffolds, Artificial Cells# Nanomedicine and Biotechnology (2017)
28. Differentiation of Endometrial Stem Cells Into Motor Neurons by the Use of Purmorphamin Small Molecule, Tehran University Medical Journal (2017)
29. Bioinspired Nanofiber Scaffold for Differentiating Bone Marrow-Derived Neural Stem Cells to Oligodendrocyte-Like Cells: Design, Fabrication, and Characterization, International Journal of Nanomedicine (2020)
30. Design and Fabrication of Poly (Glycerol Sebacate)-Based Fibers for Neural Tissue Engineering: Synthesis, Electrospinning, and Characterization, Polymers for Advanced Technologies (2019)
31. A Novel Polycaprolactone/Carbon Nanofiber Composite As a Conductive Neural Guidance Channel: An in Vitro and in Vivo Study, Progress in Biomaterials (2019)
32. Differentiation of Human Endometrial Stem Cells Into Endothelial-Like Cells on Gelatin/Chitosan/Bioglass Nanofibrous Scaffolds, Artificial Cells# Nanomedicine and Biotechnology (2016)
33. Unrestricted Somatic Stem Cells Loaded in Nanofibrous Conduit As Potential Candidate for Sciatic Nerve Regeneration, Journal of Molecular Neuroscience (2019)
34. Naturally Occurring Biological Macromolecules-Based Hydrogels: Potential Biomaterials for Peripheral Nerve Regeneration, International Journal of Biological Macromolecules (2020)
35. In Vitro Effect of Graphene Structures As an Osteoinductive Factor in Bone Tissue Engineering: A Systematic Review, Journal of Biomedical Materials Research - Part A (2018)
36. Comparison of Capability of Human Bone Marrow Mesenchymal Stem Cells and Endometrial Stem Cells to Differentiate Into Motor Neurons on Electrospun Poly(Ε-Caprolactone) Scaffold, Molecular Neurobiology (2016)
37. Hybrid Pcl/Chitosan-Peo Nanofibrous Scaffolds Incorporated With A. Euchroma Extract for Skin Tissue Engineering Application, Carbohydrate Polymers (2022)
38. Bioactive Glasses and Glass/Polymer Composites for Neuroregeneration: Should We Be Hopeful?, Applied Sciences (Switzerland) (2020)
39. Characterization of Wet-Electrospun Cellulose Acetate Based 3-Dimensional Scaffolds for Skin Tissue Engineering Applications: Influence of Cellulose Acetate Concentration, Cellulose (2016)
40. Oleic Acid Promotes the Expression of Neural Markers in Differentiated Human Endometrial Stem Cells, Journal of Chemical Neuroanatomy (2017)
41. Fabrication and in Vitro Evaluation of 3D Composite Scaffold Based on Collagen/Hyaluronic Acid Sponge and Electrospun Polycaprolactone Nanofibers for Peripheral Nerve Regeneration, Journal of Biomedical Materials Research - Part A (2021)
42. Induction of Human Umbilical Wharton’S Jelly-Derived Mesenchymal Stem Cells Toward Motor Neuron-Like Cells, In Vitro Cellular and Developmental Biology - Animal (2015)
43. Regeneration of Sciatic Nerve Crush Injury by a Hydroxyapatite Nanoparticle-Containing Collagen Type I Hydrogel, Journal of Physiological Sciences (2018)
44. Preparation of Fibrin Gel Scaffolds Containing Mwcnt/Pu Nanofibers for Neural Tissue Engineering, Journal of Biomedical Materials Research - Part A (2019)
45. Differentiation of Wharton’S Jelly-Derived Mesenchymal Stem Cells Into Motor Neuron-Like Cells on Three-Dimensional Collagen-Grafted Nanofibers, Molecular Neurobiology (2016)
46. Fabrication of Hydrogel Based Nanocomposite Scaffold Containing Bioactive Glass Nanoparticles for Myocardial Tissue Engineering, Materials Science and Engineering C (2016)
47. Synergistic Combination of Bioactive Glasses and Polymers for Enhanced Bone Tissue Regeneration, Materials Today: Proceedings (2018)
48. Potential of Bioactive Glasses for Cardiac and Pulmonary Tissue Engineering, Materials (2017)
49. Ultra-Fast Microwave-Assisted Synthesis of Diopside Nanopowder for Biomedical Applications, Ceramics International (2018)