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Stimulation of Osteogenic Differentiation of Induced Pluripotent Stem Cells (Ipscs) Using Bioactive Glasses: An in Vitro Study Publisher



Kargozar S1 ; Lotfibakhshaeish N2 ; Ebrahimibarough S2 ; Nazari B3 ; Hill RG4
Authors
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Authors Affiliations
  1. 1. Tissue Engineering Research Group, Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  2. 2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Unit of Dental Physical Sciences, Barts, London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom

Source: Frontiers in Bioengineering and Biotechnology Published:2019


Abstract

Selection and use of an optimal cell source for bone tissue engineering (BTE) remain a challenging issue; the invention of induced pluripotent stem cells (iPSCs) have created new hopes on this regard. At the present study, we attempted to show the usability of iPSCs in combination with bioactive glasses (BGs) for bone regeneration applications. For this aim, iPSCs were cultured and incubated with the strontium and cobalt-containing BGs for different intervals (1, 5, and 7 days). The cell cytotoxicity and attachment were assessed using MTT assay and scanning electron microscopy (SEM), respectively. Moreover, the osteogenic differentiation of iPSCs seeded onto the glasses was evaluated using alkaline phosphatase (ALP) activity assay and real-time PCR. The obtained results clarified that although the cell viability is decreased during a 7 day period, the iPSCs could adhere and expand onto the BGs particles and over-express the osteogenic markers, including osteocalcin, osteonectin, and Runx2. Based on the data, we conclude that iPSCs in a combination of BGs can be considered as a potential candidate for BTE strategies. © Copyright © 2019 Kargozar, Lotfibakhshaeish, Ebrahimi-Barough, Nazari and Hill.
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