Differentiation of Candida Albicans Complex Species Isolated From Invasive and Non-Invasive Infections Using Hwp1 Gene Size Polymorphism

Differentiation of Candida Albicans Complex Species Isolated From Invasive and Non-Invasive Infections Using Hwp1 Gene Size Polymorphism Publisher

Salehipour K¹ ; Aboutalebian S¹ ; Charsizadeh A² ; Ahmadi B³ ; Mirhendi H^{1, 4}

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1. Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2. Immunology, Asthma, and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Medical Laboratory Sciences, Faculty of Paramedical, Bushehr University of Medical Sciences, Bushehr, Iran
4. Core Facilities Research Laboratory, Mycology Reference Laboratory, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Current Medical Mycology Published:2021

Abstract

Background and Purpose: Taxonomy of Candida is controversial and has changed due to the investigation of the novel species. Candida africana and Candida dubliniensis are new members of the C. albicans complex that are currently gaining both clinical and epidemiologic significance. This study aimed to report the prevalence of C. africana among the strains isolated from patients using hyphal wall protein 1 (HWP1) gene size polymorphism. Materials and Methods: In total, 235 yeasts confirmed as C. albicans complex based on chromogenic media and internal transcribed spacers sequencing isolated from various clinical forms of invasive and non-invasive candidiasis mainly candidemia were re-identified using HWP1 gene polymorphisms. The HWP1polymerase chain reaction amplicons were re-confirmed by sequencing and BLAST analysis. Results: Based on the HWP1 gene size polymorphism, 223 strains were identified as C. albicans (94.89%) from which 7 isolates produced two DNA fragments (850 and 941 bp). The C. dubliniensis (n=4, 1.7%), C. africana (n=1, 0.42%), and mix of C. albicans and C. africana (n=7, 2.97%) were also identified. Conclusion: It can be said that C. albicans remains the most common Candida species, while C. dubliniensis and C. africana are rarely found among the patient isolates. Due to limited information on the molecular epidemiology of this novel yeast, more studies using molecular methods are recommended. © 2021 Mazandaran University of Medical Sciences. All rights reserved.

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Bahram Nikmanesh (3)

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Style	Citing Format
MLA	Salehipour K, et al.. "Differentiation of Candida Albicans Complex Species Isolated From Invasive and Non-Invasive Infections Using Hwp1 Gene Size Polymorphism." Current Medical Mycology, vol. 7, no. 2, 2021, pp. 34-38.
APA	Salehipour K, Aboutalebian S, Charsizadeh A, Ahmadi B, Mirhendi H (2021). Differentiation of Candida Albicans Complex Species Isolated From Invasive and Non-Invasive Infections Using Hwp1 Gene Size Polymorphism. Current Medical Mycology, 7(2), 34-38.
Chicago	Salehipour K, Aboutalebian S, Charsizadeh A, Ahmadi B, Mirhendi H. "Differentiation of Candida Albicans Complex Species Isolated From Invasive and Non-Invasive Infections Using Hwp1 Gene Size Polymorphism." Current Medical Mycology 7, no. 2 (2021): 34-38.
Harvard	Salehipour K et al. (2021) 'Differentiation of Candida Albicans Complex Species Isolated From Invasive and Non-Invasive Infections Using Hwp1 Gene Size Polymorphism', Current Medical Mycology, 7(2), pp. 34-38.
Vancouver	Salehipour K, Aboutalebian S, Charsizadeh A, Ahmadi B, Mirhendi H. Differentiation of Candida Albicans Complex Species Isolated From Invasive and Non-Invasive Infections Using Hwp1 Gene Size Polymorphism. Current Medical Mycology. 2021;7(2):34-38.
BibTex	@article{ author = {Salehipour K and Aboutalebian S and Charsizadeh A and Ahmadi B and Mirhendi H}, title = {Differentiation of Candida Albicans Complex Species Isolated From Invasive and Non-Invasive Infections Using Hwp1 Gene Size Polymorphism}, journal = {Current Medical Mycology}, volume = {7}, number = {2}, pages = {34-38}, year = {2021} }
RIS	TY - JOUR AU - Salehipour K AU - Aboutalebian S AU - Charsizadeh A AU - Ahmadi B AU - Mirhendi H TI - Differentiation of Candida Albicans Complex Species Isolated From Invasive and Non-Invasive Infections Using Hwp1 Gene Size Polymorphism JO - Current Medical Mycology VL - 7 IS - 2 SP - 34 EP - 38 PY - 2021 ER -

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