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Evaluation of Ros-Tac Score and Dna Damage in Fertile Normozoospermic and Infertile Asthenozoospermic Males Pubmed



Vatannejad A1, 2 ; Tavilani H3 ; Sadeghi MR4 ; Amanpour S5 ; Shapourizadeh S6 ; Doosti M1
Authors
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Authors Affiliations
  1. 1. Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Student's Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Urology and nephrology research center, Hamadan University of Medical Sciences, Hamadan, Iran
  4. 4. Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
  5. 5. Cancer biology research Centre, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Payame Noor University, Tehran, Iran

Source: Urology Journal Published:2017


Abstract

Purpose: The aim of study was to evaluate reactive oxygen species (ROS), total antioxidant capacity (TAC) and ROS-TAC score as indicator for oxidative stress status as well as 8-hydrodeoxyguanosine (8-OHdG) levels as a marker for DNA damage in the seminal plasma of asthenozoospermia patients compared to normozoospermia samples. Materials and Methods: The semen samples of 28 fertile normozoospermic donors and 25 infertile men with asthenozoospermia were analyzed according to World Health Organization (WHO) criteria. ROS production was measured in neat semen samples by the chemiluminescent assay. Plasma levels of TAC was measured by commercially available colorimetric assays. The levels of DNA oxidative damage were measured by seminal plasma levels of 8-OHdG using ELISA method. ROS-TAC score was measured using principal component analysis. Results: Asthenozoospermic men had a higher ROS levels compared to the normozoospermic men (P = .01). However, no significant difference was observed in TAC levels between the groups. ROS-TAC score in asthenozoospermic men was lower than normozoospermic men (P = .02). The levels of 8-OHdG in the asthenozoospermic men were higher than normozoospermic men (P = .01). Conclusion: The present study demonstrated a decrease in ROS-TAC score and, a high DNA damage in asthenozoospermia compared to normozoospermia. ROS-TAC score can predict the oxidative damage of semen samples of astenozoospermic infertile males.
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