Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Unrestricted Somatic Stem Cells Loaded in Nanofibrous Conduit As Potential Candidate for Sciatic Nerve Regeneration Publisher Pubmed



Farzamfar S1 ; Ehterami A2 ; Salehi M3, 4 ; Vaeez A1 ; Atashi A4, 5 ; Sahrapeyma H6
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Mechanical and Aerospace Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
  3. 3. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
  4. 4. Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
  5. 5. Department of Hematology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
  6. 6. Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran

Source: Journal of Molecular Neuroscience Published:2019


Abstract

Motor and sensory recovery following critical size peripheral nerve defects is often incomplete. Although nerve grafting has been proposed as the gold standard, it is associated with several disadvantages. Here we report a novel approach to peripheral nerve repair using Human Unrestricted Somatic Stem Cells (USSC) delivered through an electrospun neural guidance conduit. Conduits were produced from PCL and gelatin blend. Several in vitro methods were utilized to investigate the conduit’s physicochemical and biological characteristics. Nerve regeneration was studied across a 10-mm sciatic nerve gap in Wistar rats. For functional analysis, the conduits were seeded with 3 × 10 4 USSCs and implanted into a 10-mm sciatic nerve defect. After 14 weeks, the results of functional recovery analysis and histopathological examinations showed that animals implanted with USSC containing conduits exhibited improved functional and histopathological recovery which was more close to the autograft group compared to other groups. Our results support the potential applicability of USSCs to treat peripheral nerve injury in the clinic. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
Related Docs
View other Related Docs
1. A Novel Polycaprolactone/Carbon Nanofiber Composite As a Conductive Neural Guidance Channel: An in Vitro and in Vivo Study, Progress in Biomaterials (2019)
2. Polyurethane/Gelatin Nanofibrils Neural Guidance Conduit Containing Platelet-Rich Plasma and Melatonin for Transplantation of Schwann Cells, Cellular and Molecular Neurobiology (2018)
3. Naturally Occurring Biological Macromolecules-Based Hydrogels: Potential Biomaterials for Peripheral Nerve Regeneration, International Journal of Biological Macromolecules (2020)
4. Neural Tissue Regeneration by a Gabapentin-Loaded Cellulose Acetate/Gelatin Wet-Electrospun Scaffold, Cellulose (2018)
5. Fabrication of Poly(L-Lactic Acid)/Chitosan Scaffolds by Solid-Liquid Phase Separation Method for Nerve Tissue Engineering: An in Vitro Study on Human Neuroblasts, Journal of Craniofacial Surgery (2019)
6. Electrospun Nerve Guide Scaffold of Poly(Ε-Caprolactone)/Collagen/Nanobioglass: An in Vitro Study in Peripheral Nerve Tissue Engineering, Journal of Biomedical Materials Research - Part A (2017)
7. Polycaprolactone/Gelatin Nanofibrous Scaffolds for Tissue Engineering, Biointerface Research in Applied Chemistry (2021)
8. Sciatic Nerve Regeneration by Transplantation of Schwann Cells Via Erythropoietin Controlled-Releasing Polylactic Acid/Multiwalled Carbon Nanotubes/Gelatin Nanofibrils Neural Guidance Conduit, Journal of Biomedical Materials Research - Part B Applied Biomaterials (2018)
Experts (# of related papers)
View all Related Experts
Somayeh Ebrahimi Barough (19)
Mahmoud Azami (5)
Other Related Docs
9. Preparation and Characterization of Pcl Polymeric Scaffolds Coated With Chitosan/ Bioactive Glass/Gelatin Nanoparticles Using the Tips Methodology for Bone Tissue Engineering, Nanomedicine Journal (2019)
10. Concurrent Application of Conductive Biopolymeric Chitosan/ Polyvinyl Alcohol/ Mwcnts Nanofibers, Intracellular Signaling Manipulating Molecules and Electrical Stimulation for More Effective Cardiac Tissue Engineering, Materials Chemistry and Physics (2021)
11. Prospects of Peripheral Nerve Tissue Engineering Using Nerve Guide Conduits Based on Silk Fibroin Protein and Other Biopolymers, International Materials Reviews (2017)
12. Bioengineered Scaffolds for Stem Cell Applications in Tissue Engineering and Regenerative Medicine, Advances in Experimental Medicine and Biology (2018)
13. Improvement of Sciatic Nerve Regeneration by Multichannel Nanofibrous Membrane-Embedded Electro-Conductive Conduits Functionalized With Laminin, Journal of Materials Science: Materials in Medicine (2022)
14. In Vitro Differentiation of Human Ips Cells Into Neural Like Cells on a Biomimetic Polyurea, Molecular Neurobiology (2017)
15. Biomaterials for Regenerative Medicine: Historical Perspectives and Current Trends, Advances in Experimental Medicine and Biology (2018)
16. Taurine-Loaded Poly (Ε-Caprolactone)/Gelatin Electrospun Mat As a Potential Wound Dressing Material: In Vitro and in Vivo Evaluation, Journal of Bioactive and Compatible Polymers (2018)
17. Conductive Biomaterials As Nerve Conduits: Recent Advances and Future Challenges, Applied Materials Today (2020)
18. In Vitro and in Vivo Studies of Biaxially Electrospun Poly(Caprolactone)/Gelatin Nanofibers, Reinforced With Cellulose Nanocrystals, for Wound Healing Applications, Cellulose (2020)
19. Electrospun Cellulose Acetate/Gelatin Nanofibrous Wound Dressing Containing Berberine for Diabetic Foot Ulcer Healing: In Vitro and in Vivo Studies, Scientific Reports (2020)
20. Bioinspired Nanofiber Scaffold for Differentiating Bone Marrow-Derived Neural Stem Cells to Oligodendrocyte-Like Cells: Design, Fabrication, and Characterization, International Journal of Nanomedicine (2020)
21. In Vitro and in Vivo Study of Pcl/Coll Wound Dressing Loaded With Insulin-Chitosan Nanoparticles on Cutaneous Wound Healing in Rats Model, International Journal of Biological Macromolecules (2018)
22. Characterization of Wet-Electrospun Cellulose Acetate Based 3-Dimensional Scaffolds for Skin Tissue Engineering Applications: Influence of Cellulose Acetate Concentration, Cellulose (2016)
23. Bridging Potential of Taurine-Loading Pcl Conduits Transplanted With Henscs on Resected Sciatic Nerves, Regenerative Therapy (2022)
24. Design and Fabrication of Poly (Glycerol Sebacate)-Based Fibers for Neural Tissue Engineering: Synthesis, Electrospinning, and Characterization, Polymers for Advanced Technologies (2019)
25. Effect of Mesenchymal Stem Cells-Derived Exosomes on Tumor Microenvironment: Tumor Progression Versus Tumor Suppression, Journal of Cellular Physiology (2019)
26. Evaluation Resistance Levels of the Pcl/Gt Nanofiber Mats During Exposure to Pahs for Use in the Occupational Setting, SN Applied Sciences (2019)
27. Design and Fabrication of a Nanofibrous Polycaprolactone Tubular Nerve Guide for Peripheral Nerve Tissue Engineering Using a Two-Pole Electrospinning System, Biomedical Materials (Bristol) (2016)
28. Developed Methods for the Preparation of Electrospun Nanofibers Containing Plant-Derived Oil or Essential Oil: A Systematic Review, Polymer Bulletin (2020)
29. Regeneration of Sciatic Nerve Injury Through Nanofiber Neural Guidance Channels Containing Collagen Hydrogel and Acetyl L Carnitine: An in Vitro and in Vivo Study, Journal of Bioactive and Compatible Polymers (2023)
30. Sciatic Nerve Regeneration With Collagen Type I Hydrogel Containing Chitosan Nanoparticle Loaded by Insulin, International Journal of Polymeric Materials and Polymeric Biomaterials (2019)
31. Development of New Nanofibrous Nerve Conduits by Pcl-Chitosan-Hyaluronic Acid Containing Piracetam-Vitamin B12 for Sciatic Nerve: A Rat Model, International Journal of Pharmaceutics (2024)
32. In Vitro Comparative Survey of Cell Adhesion and Proliferation of Human Induced Pluripotent Stem Cells on Surfaces of Polymeric Electrospun Nanofibrous and Solution-Cast Film Scaffolds, Journal of Biomedical Materials Research - Part A (2015)
33. Unrestricted Somatic Stem Cells, As a Novel Feeder Layer: Ex Vivo Culture of Human Limbal Stem Cells, Journal of Cellular Biochemistry (2018)
34. Design and Characterization of Biodegradable Multi Layered Electrospun Nanofibers for Corneal Tissue Engineering Applications, Journal of Biomedical Materials Research - Part A (2019)
35. Fabrication and in Vitro Evaluation of 3D Composite Scaffold Based on Collagen/Hyaluronic Acid Sponge and Electrospun Polycaprolactone Nanofibers for Peripheral Nerve Regeneration, Journal of Biomedical Materials Research - Part A (2021)
36. Extracellular Micro/Nanovesicles Rescue Kidney From Ischemia-Reperfusion Injury, Journal of Cellular Physiology (2019)
37. Enhanced Sciatic Nerve Regeneration by Poly-L-Lactic Acid/Multi-Wall Carbon Nanotube Neural Guidance Conduit Containing Schwann Cells and Curcumin Encapsulated Chitosan Nanoparticles in Rat, Materials Science and Engineering C (2020)
38. Encapsulation of Curcumin Loaded Chitosan Nanoparticle Within Poly (Ε-Caprolactone) and Gelatin Fiber Mat for Wound Healing and Layered Dermal Reconstitution, International Journal of Biological Macromolecules (2020)
39. Cerium Oxide Nanoparticle-Containing Poly (Ε-Caprolactone)/Gelatin Electrospun Film As a Potential Wound Dressing Material: In Vitro and in Vivo Evaluation, Materials Science and Engineering C (2017)
40. Human Unrestricted Somatic Stem Cells Ameliorate Sepsis-Related Acute Lung Injury in Mice, Journal of Cellular Physiology (2019)
41. Fabrication and Evaluation of Porous and Conductive Nanofibrous Scaffolds for Nerve Tissue Engineering, Journal of Materials Science: Materials in Medicine (2021)
42. Sophisticated Polycaprolactone/Gelatin Nanofibrous Nerve Guided Conduit Containing Platelet-Rich Plasma and Citicoline for Peripheral Nerve Regeneration: In Vitro and in Vivo Study, International Journal of Biological Macromolecules (2020)
43. Preparation of Fibrin Gel Scaffolds Containing Mwcnt/Pu Nanofibers for Neural Tissue Engineering, Journal of Biomedical Materials Research - Part A (2019)
44. Tissue-Engineered Core-Shell Silk-Fibroin/Poly- L -Lactic Acid Nerve Guidance Conduit Containing Encapsulated Exosomes of Human Endometrial Stem Cells Promotes Peripheral Nerve Regeneration, ACS Biomaterials Science and Engineering (2023)
45. Erythropoietin/Aloe Vera-Releasing Wet-Electrospun Polyvinyl Alcohol/Chitosan Sponge-Like Wound Dressing: In Vitro and in Vivo Studies, Journal of Bioactive and Compatible Polymers (2018)
46. Osteoconduction of Unrestricted Somatic Stem Cells on an Electrospun Polylactic-Co-Glycolic Acid Scaffold Coated With Nanohydroxyapatite, Cells Tissues Organs (2018)
47. An Investigation of Electrospun Henna Leaves Extract-Loaded Chitosan Based Nanofibrous Mats for Skin Tissue Engineering, Materials Science and Engineering C (2017)
48. Recent Approaches to Enhance Osteogenesis of Dental Pulp Stem Cells on Electrospun Scaffolds, Current Stem Cell Research and Therapy (2024)
49. Reinforcing Mechanical Strength of Electrospun Chitosan Nanofibrous Scaffold Using Cellulose Nanofibers, Journal of Nano Research (2018)
50. The Therapeutic Potential of Mesenchymal Stem Cell–Derived Exosomes in Treatment of Neurodegenerative Diseases, Molecular Neurobiology (2019)