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Antimicrobial Photodynamic Therapy: Modern Technology in the Treatment of Wound Infections in Patients With Burns Publisher Pubmed



Mahmoudi H1 ; Pourhajibagher M2 ; Chiniforush N3 ; Alikhani MY1 ; Bahador A4
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran
  2. 2. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Oral Microbiology Laboratory, Department of Microbiology, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Wound Care Published:2023


Abstract

Background: Staphylococcus aureus is one of the most frequently isolated microorganisms from burn wounds. Antimicrobial photodynamic therapy (aPDT) is a new strategy that may improve antimicrobial treatment. Method: This study evaluated three meticillin-resistant Staphylococcus aureus (MRSA) and three meticillin-sensitive Staphylococcus aureus (MSSA) clinical isolates, which produced a biofilm with 0.1mg/ml Toluidine Blue O (TBO) (Sigma-Aldrich, Germany) with an energy density of 45J/cm2 and 90J/cm2, for MRSA and MSSA, respectively. The antibiofilm potential of aPDT with TBO was analysed using crystal violet assays and scanning electron microscopy. Results: TBO-aPDT significantly degraded the biofilm formed by MRSA and MSSA clinical isolates (p < 0.05). Conclusion: Our results indicated that aPDT is an effective approach to combat bacterial biofilms associated with burn wound infection. aPDT could provide a supplemental to the treatment of wound and tissue infection, and patients with burns may benefit from combined treatments. Declaration of interest: This research was supported by Vice Chancellor for Research & Technology of Hamadan University of Medical Sciences, Hamadan, IRAN (Grant no: 960129387). The authors have no conflicts of interest to declare. 2023 MA Healthcare Ltd. © 2023 MA Healthcare Ltd. All rights reserved.
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