Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Mesenchymal Stem Cell-Derived Extracellular Vesicles Alone or in Conjunction With a Sdkp-Conjugated Self-Assembling Peptide Improve a Rat Model of Myocardial Infarction Publisher Pubmed



Firoozi S1, 2 ; Pahlavan S3 ; Ghanian MH2 ; Rabbani S4 ; Barekat M5 ; Nazari A3 ; Pakzad M3 ; Shekari F3, 7 ; Hassani SN3 ; Moslem F3 ; Lahrood FN3 ; Soleimani M6 ; Baharvand H3, 7
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran
  2. 2. Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
  3. 3. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
  4. 4. Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Regenerative Medicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
  6. 6. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Developmental Biology, University of Science and Culture, Tehran, Iran

Source: Biochemical and Biophysical Research Communications Published:2020


Abstract

Purpose: The aim of this study was to investigate the cardiac repair effect of human bone marrow mesenchymal stromal cells-derived extracellular vesicles (MSC-EVs) after intramyocardial injection in free form or encapsulated within a self-assembling peptide hydrogel modified with SDKP motif, in a rat model of myocardial infarction (MI). Methods: MSC-EVs were isolated by ultracentrifuge and characterized for physical parameters and surface proteins. Furthermore, cellular uptake and cardioprotective effects of MSC-EVs were evaluated in vitro using neonatal mouse cardiomyocytes (NMCMs). In vivo effects of MSC-EVs on cardiac repair were studied in rat MI model by comparing the vehicle group (injected with PBS), EV group (injected with MSC-EVs) and Gel + EV group (injected with MSC-EVs encapsulated in (RADA)4-SDKP hydrogel) with respect to cardiac function and fibrotic area using echocardiography and Masson's trichrome staining, respectively. Histological sections were assessed by α-SMA and CD68 immunostaining to investigate the angiogenic and anti-inflammatory effects of the MSC-EVs. Results: We observed the uptake of MSC-EVs into NMCMs which led to NMCMs protection against H2O2-induced oxidative stress by substantial reduction of apoptosis. In myocardial infarcted rats, cardiac function was improved after myocardial injection of MSC-EVs alone or in conjunction with (RADA)4-SDKP hydrogel. This functional restoration coincided with promotion of angiogenesis and decrement of fibrosis and inflammation. Conclusion: These data demonstrated that MSC-EVs can be used alone as a potent therapeutic agent for improvement of myocardial infarction. © 2020 Elsevier Inc.
Related Docs
View other Related Docs
1. A Cell-Free Sdkp-Conjugated Self-Assembling Peptide Hydrogel Sufficient for Improvement of Myocardial Infarction, Biomolecules (2020)
2. Effectiveness of Exosome Mediated Mir-126 and Mir-146A Delivery on Cardiac Tissue Regeneration, Cell and Tissue Research (2022)
3. Mesenchymal Stem Cell-Derived Exosomes: A New Therapeutic Approach to Osteoarthritis?, Stem Cell Research and Therapy (2019)
4. Hydrogel-Mediated Sustained Systemic Delivery of Mesenchymal Stem Cell-Derived Extracellular Vesicles Improves Hepatic Regeneration in Chronic Liver Failure, ACS Applied Materials and Interfaces (2019)
5. Extracellular Micro/Nanovesicles Rescue Kidney From Ischemia-Reperfusion Injury, Journal of Cellular Physiology (2019)
6. Mesenchymal Stem Cells-Derived Exosomes for Wound Regeneration, Advances in Experimental Medicine and Biology (2018)
7. Exosomes, Autophagy and Er Stress Pathways in Human Diseases: Cross-Regulation and Therapeutic Approaches, Biochimica et Biophysica Acta - Molecular Basis of Disease (2022)
8. Advanced Micro-/Nanotechnologies for Exosome Encapsulation and Targeting in Regenerative Medicine, Clinical and Experimental Medicine (2023)
Experts (# of related papers)
View all Related Experts
Shahram Rabbani (5)
Bagher Larijani (3)
Other Related Docs
9. Extracellular Vesicles Derived From Human Es-Mscs Protect Retinal Ganglion Cells and Preserve Retinal Function in a Rodent Model of Optic Nerve Injury, Stem Cell Research and Therapy (2020)
10. The Relationship Between Molecular Content of Mesenchymal Stem Cells Derived Exosomes and Their Potentials: Opening the Way for Exosomes Based Therapeutics, Biochimie (2019)
11. A Paradigm Shift in Cell-Free Approach: The Emerging Role of Mscs-Derived Exosomes in Regenerative Medicine, Journal of Translational Medicine (2021)
12. Epigenetic Regulation in Myocardial Infarction: Non-Coding Rnas and Exosomal Non-Coding Rnas, Frontiers in Cardiovascular Medicine (2022)
13. Improvement, Scaling-Up, and Downstream Analysis of Exosome Production, Critical Reviews in Biotechnology (2020)
14. Exosome Loaded Alginate Hydrogel Promotes Tissue Regeneration in Full-Thickness Skin Wounds: An in Vivo Study, Journal of Biomedical Materials Research - Part A (2020)
15. Effects of Mesenchymal Stem Cell-Derived Exosomes on Angiogenesis in Regenerative Medicine, Current Regenerative Medicine (2017)
16. Contribution of Extracellular Vesicles and Molecular Chaperones in Age-Related Neurodegenerative Disorders of the Cns, International Journal of Molecular Sciences (2023)
17. Communication Between Stromal and Hematopoietic Stem Cell by Exosomes in Normal and Malignant Bone Marrow Niche, Biomedicine and Pharmacotherapy (2020)
18. Cardiac Tissue Regeneration by Microfluidic Generated Cardiac Cell-Laden Calcium Alginate Microgels and Mesenchymal Stem Cell Extracted Exosomes on Myocardial Infarction Model, International Journal of Biological Macromolecules (2025)
19. Whole-Heart Tissue Engineering and Cardiac Patches: Challenges and Promises, Bioengineering (2023)
20. The Safety and Efficacy of Adipose Tissue-Derived Exosomes in Treating Mild to Moderate Plaque Psoriasis: A Clinical Study, Life Sciences (2024)
21. Designer Exosomes: A New Platform for Biotechnology Therapeutics, BioDrugs (2020)
22. Amphotericin B-Loaded Extracellular Vesicles Derived From Leishmania Major Enhancing Cutaneous Leishmaniasis Treatment Through in Vitro and in Vivo Studies, Iranian Journal of Parasitology (2023)
23. Cell Type-Specific Extracellular Vesicles and Their Impact on Health and Disease, International Journal of Molecular Sciences (2024)
24. Effect of Circulating Exosomes Derived From Normal-Weight and Obese Women on Gluconeogenesis, Glycogenesis, Lipogenesis and Secretion of Fgf21 and Fetuin a in Hepg2 Cells, Diabetology and Metabolic Syndrome (2020)
25. Sirolimus-Loaded Exosomes As a Promising Vascular Delivery System for the Prevention of Post-Angioplasty Restenosis, Drug Delivery and Translational Research (2024)
26. Novel Cell-Based Therapies in Hepatic Disorders, Journal of Mazandaran University of Medical Sciences (2020)
27. Exosomes As a Next-Generation Drug Delivery System: An Update on Drug Loading Approaches, Characterization, and Clinical Application Challenges, Acta Biomaterialia (2020)
28. Extracellular Vesicles in Acute Leukemia: A Mesmerizing Journey With a Focus on Transferred Micrornas, Frontiers in Cell and Developmental Biology (2021)
29. Extracellular Vesicles in Bone and Periodontal Regeneration: Current and Potential Therapeutic Applications, Cell and Bioscience (2021)
30. Effect of Photobiomodulation on Structure and Function of Extracellular Vesicle Secreted From Mesenchymal Stem Cells, Photochemistry and Photobiology (2022)
31. Effects of Neural Stem Cell-Derived Extracellular Vesicles on Neuronal Protection and Functional Recovery in the Rat Model of Middle Cerebral Artery Occlusion, Cell Biochemistry and Function (2020)
32. Micrornas and Exosomes: Key Players in Hiv Pathogenesis, HIV Medicine (2020)
33. Development and Application of Mesenchymal Stem Cell-Derived Exosomes in Cartilage Tissue Repair, Journal of Military Medicine (2022)
34. Microfluidics for Detection of Exosomes and Micrornas in Cancer: State of the Art, Molecular Therapy Nucleic Acids (2022)
35. The Therapeutic Potential of Mesenchymal Stem Cell–Derived Exosomes in Treatment of Neurodegenerative Diseases, Molecular Neurobiology (2019)
36. Role of Non-Coding Rnas in Neuroblastoma, Cancer Gene Therapy (2023)
37. Regenerative Medicine for the Treatment of Ischemic Heart Disease; Status and Future Perspectives, Frontiers in Cell and Developmental Biology (2021)
38. Localized Administration of Mesenchymal Stem Cell-Derived Exosomes for the Treatment of Refractory Perianal Fistula in Patients With Crohn's Disease: A Phase Ii Clinical Trial, Diseases of the Colon and Rectum (2024)
39. Increasing the Sensitivity and Accuracy of Detecting Exosomes As Biomarkers for Cancer Monitoring Using Optical Nanobiosensors, Cancer Cell International (2024)
40. Detailed Role of Mesenchymal Stem Cell (Msc)-Derived Exosome Therapy in Cardiac Diseases, EXCLI Journal (2024)
41. Therapeutic Role of Mesenchymal Stem Cell-Derived Exosomes in Respiratory Disease, Stem Cell Research and Therapy (2022)
42. Recent Advances in Porphyrin-Based Nanocomposites for Effective Targeted Imaging and Therapy, Biomaterials (2020)
43. Application of Stem Cell-Derived Exosomes in Anterior Segment Eye Diseases: A Comprehensive Update Review, Ocular Surface (2025)
44. Exosomes As a Novel Cell-Free Therapeutic Approach in Gastrointestinal Diseases, Journal of Cellular Physiology (2019)
45. The Footprint of Exosomes in the Radiation-Induced Bystander Effects, Bioengineering (2022)
46. Mesenchymal Stem/Stromal Cell-Derived Exosomes in Regenerative Medicine and Cancer; Overview of Development, Challenges, and Opportunities, Stem Cell Research and Therapy (2021)
47. Preclinical and Clinical Studies on the Use of Extracellular Vesicles Derived From Mesenchymal Stem Cells in the Treatment of Chronic Wounds, Molecular Pharmaceutics (2024)
48. Emerging Role of Microbiota Derived Outer Membrane Vesicles to Preventive, Therapeutic and Diagnostic Proposes, Infectious Agents and Cancer (2023)
49. Exosomal Micrornas in Regulation of Tumor Cells Resistance to Apoptosis, Biochemistry and Biophysics Reports (2024)
50. Extracellular Vesicles Biogenesis, Isolation, Manipulation and Genetic Engineering for Potential in Vitro and in Vivo Therapeutics: An Overview, Frontiers in Bioengineering and Biotechnology (2022)