Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Tissue-Engineered Regeneration of Hemisected Spinal Cord Using Human Endometrial Stem Cells, Poly Ε-Caprolactone Scaffolds, and Crocin As a Neuroprotective Agent Publisher Pubmed



Terraf P1 ; Kouhsari SM1 ; Ai J2 ; Babaloo H2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Cellular and Molecular Biology, School of Biology, University College of Science, University of Tehran, Tehran, Iran
  2. 2. Department of Tissue Engineering, Faculty of Advanced Medical Technologies, Tehran University of Medical Sciences and Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Molecular Neurobiology Published:2017


Abstract

Loss of motor and sensory function as a result of neuronal cell death and axonal degeneration are the hallmarks of spinal cord injury. To overcome the hurdles and achieve improved functional recovery multiple aspects, it must be taken into account. Tissue engineering approaches by coalescing biomaterials and stem cells offer a promising future for treating spinal cord injury. Here we investigated human endometrial stem cells (hEnSCs) as our cell source. Electrospun poly ε-caprolactone (PCL) scaffolds were used for hEnSC adhesion and growth. Scanning electron microscopy (SEM) confirmed the attachment and survival of stem cells on the PCL scaffolds. The scaffold-stem cell construct was transplanted into the hemisected spinal cords of adult male rats. Crocin, an ethanol-extractable component of Crocus sativus L., was administered to rats for 15 consecutive days post injury. Neurite outgrowth and axonal regeneration were investigated using immunohistochemical staining for neurofilament marker NF-H and luxol-fast blue (LFB) staining, respectively. TNF-α staining was performed to determine the inflammatory response in each group. Functional recovery was assessed via the Basso-Beattie-Bresnahan (BBB) scale. Results showed that PCL scaffolds seeded with hEnSCs restored the continuity of the damaged spinal cord and decreased cavity formation. Additionally, hEnSC-seeded scaffolds contributed to the functional recovery of the spinal cord. Hence, hEnSC-seeded PCL scaffolds may serve as promising transplants for spinal cord tissue engineering purposes. Furthermore, crocin had an augmenting effect on spinal cord regeneration and proved to exert neuroprotective effects on damaged neurons and may be further studied as a promising drug for spinal cord injury. © 2016, Springer Science+Business Media New York.
Related Docs
View other Related Docs
1. Pcl/Gelatin Nanofibrous Scaffolds With Human Endometrial Stem Cells/Schwann Cells Facilitate Axon Regeneration in Spinal Cord Injury, Journal of Cellular Physiology (2019)
2. Repair of Injured Spinal Cord Using Platelet-Rich Plasma- and Endometrial Stem Cells-Loaded Chitosan Scaffolds, International Journal of Polymeric Materials and Polymeric Biomaterials (2021)
3. Novel Neuroprotective Potential of Crocin in Neurodegenerative Disorders: An Illustrated Mechanistic Review, Neurochemical Research (2020)
4. Mesenchymal Endometrial Stem/Stromal Cells for Hard Tissue Engineering: A Review of in Vitro and in Vivo Evidence, Regenerative Medicine (2017)
5. Neural Stem/Progenitor Cell Transplantation for Spinal Cord Injury Treatment; a Systematic Review and Meta-Analysis, Neuroscience (2016)
6. Subventricular Zone-Derived Extracellular Vesicles Promote Functional Recovery in Rat Model of Spinal Cord Injury by Inhibition of Nlrp3 Inflammasome Complex Formation, Metabolic Brain Disease (2020)
7. Activation and Regulation of Nlrp3 Inflammasome by Intrathecal Application of Sdf-1A in a Spinal Cord Injury Model, Molecular Neurobiology (2016)
8. Application of Saffron As a Neuroprotective Agent, Saffron: Science# Technology and Health (2020)
Experts (# of related papers)
View all Related Experts
Somayeh Ebrahimi Barough (5)
Seyed Mahdi Rezayat Sorkhabadi (4)
Other Related Docs
9. Evaluation of Motor Neuron Differentiation Potential of Human Umbilical Cord Blood- Derived Mesenchymal Stem Cells, in Vitro, Journal of Chemical Neuroanatomy (2017)
10. Differentiation of Wharton’S Jelly-Derived Mesenchymal Stem Cells Into Motor Neuron-Like Cells on Three-Dimensional Collagen-Grafted Nanofibers, Molecular Neurobiology (2016)
11. Endometrial and Menstrual Blood Mesenchymal Stem/Stromal Cells: Biological Properties and Clinical Application, Frontiers in Cell and Developmental Biology (2020)
12. Inhibitor of Pi3k/Akt Signaling Pathway Small Molecule Promotes Motor Neuron Differentiation of Human Endometrial Stem Cells Cultured on Electrospun Biocomposite Polycaprolactone/Collagen Scaffolds, Molecular Neurobiology (2017)
13. Combined Application of Neural Stem/Progenitor Cells and Scaffolds on Locomotion Recovery Following Spinal Cord Injury in Rodents: A Systematic Review and Meta-Analysis, Neurosurgical Review (2022)
14. Mechano-Transduction Signals Derived From Self-Assembling Peptide Nanofibers Containing Long Motif of Laminin Influence Neurogenesis in In-Vitro and In-Vivo, Molecular Neurobiology (2017)
15. Crocin May Be Useful to Prevent or Treatment of Alcohol Induced Neurodegeneration and Neurobehavioral Sequels Via Modulation of Creb/Bdnf and Akt/Gsk Signaling Pathway, Medical Hypotheses (2019)
16. Human Embryonic Stem Cell-Derived Neural Stem Cells Encapsulated in Hyaluronic Acid Promotes Regeneration in a Contusion Spinal Cord Injured Rat, International Journal of Biological Macromolecules (2020)
17. The Effect of Noggin Supplementation in Matrigel Nanofiber-Based Cell Culture System for Derivation of Neural-Like Cells From Human Endometrial-Derived Stromal Cells, Journal of Biomedical Materials Research - Part A (2015)
18. Self-Assembling Peptide Nanofiber Containing Long Motif of Laminin Induces Neural Differentiation, Tubulin Polymerization, and Neurogenesis: In Vitro, Ex Vivo, and in Vivo Studies, Molecular Neurobiology (2016)
19. Combined Effects of Rat Schwann Cells and 17Β-Estradiol in a Spinal Cord Injury Model, Metabolic Brain Disease (2018)
20. Saffron As a Promising Therapy for Diabetes and Alzheimer's Disease: Mechanistic Insights, Metabolic Brain Disease (2023)
21. Sumatriptan Improves the Locomotor Activity and Neuropathic Pain by Modulating Neuroinflammation in Rat Model of Spinal Cord Injury, Neurological Research (2021)
22. Prospects of Saffron and Its Derivatives in Alzheimer's Disease, Archives of Iranian Medicine (2021)
23. Injectable Alginate Chitosan Hydrogel As a Promising Bioengineered Therapy for Acute Spinal Cord Injury, Scientific Reports (2024)
24. Acute Transplantation of Human Olfactory Mucosa-Derived Olfactory Ensheathing Cells Fails to Improve Locomotor Recovery in Rats, Acta Medica Iranica (2018)
25. Metformin’S Therapeutic Potential in Spinal Cord Injury: A Systematic Review and Meta-Analysis on Locomotor Recovery, Neuropathic Pain Alleviation, and Modulation of Secondary Injury Mechanisms, Acta Neurochirurgica (2025)
26. Evaluating the Anti-Neuropathic Effects of the Thymol-Loaded Nanofibrous Scaffold in a Rat Model of Spinal Cord Injury, Frontiers in Pharmacology (2025)
27. Pcl/Gelatin Scaffolds and Beta-Boswellic Acid Synergistically Increase the Efficiency of Cgr8 Stem Cells Differentiation Into Dopaminergic Neuron: A New Paradigm of Parkinson's Disease Cell Therapy, Journal of Biomedical Materials Research - Part A (2021)
28. Chimeric Self-Assembling Nanofiber Containing Bone Marrow Homing Peptide’S Motif Induces Motor Neuron Recovery in Animal Model of Chronic Spinal Cord Injury; an in Vitro and in Vivo Investigation, Molecular Neurobiology (2016)
29. Bridging Potential of Taurine-Loading Pcl Conduits Transplanted With Henscs on Resected Sciatic Nerves, Regenerative Therapy (2022)
30. Reversal Effects of Crocin on Amyloid Β-Induced Memory Deficit: Modification of Autophagy or Apoptosis Markers, Pharmacology Biochemistry and Behavior (2015)