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Rapid Determination of Ethanol in Non-Alcoholic Malt Beverage by Atr-Ft-Ir Spectroscopy and Headspace Gas Chromatography Confirmation



Mazdeh FZ1 ; Chalipour A2 ; Salami F1 ; Amini M3 ; Adli H1 ; Rostami A1 ; Germi SR2 ; Atigh MNQ1 ; Hajimahmoodi M1
Authors
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Authors Affiliations
  1. 1. Drug and Food Control Department, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Food and Drug Administration, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medical Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Agricultural Science and Technology Published:2023

Abstract

Malt beverage is one of the most popular drinks in the world. Recently, consumption of non-alcoholic beverages has expanded significantly in many countries. The permitted level of alcohol in malt beverage is less than 0.5% by Iranian National Standards. In this study, a method was developed to determine ethanol content in malt beverages by FTIR, equipped with Horizontal Attenuated Total Reflectance (H-ATR). Here, the Limit Of Detection (LOD) and Limit Of Quantification (LOQ) were 0.07% and 0.23%, respectively. The correlation coefficient of calibration curve was higher than 0.999. Fifty commercial malt beverages from six brands (five Iranian and one imported brand) and three types of flavored malt beverage (classic, equatorial, and lemon) were assessed. The average detected ethanol amount in samples was 0.19%, varying between 0.00-1.47percent. The results showed that the amount of ethanol in lemon malt drinks was more than the maximum permitted limit; that could be attributed to the usage of ethylene glycol for extraction of lemon flavor. © 2023, Tarbiat Modares University. All rights reserved.