A Facile Two Step Heat Treatment Strategy for Development of Bioceramic Scaffolds for Hard Tissue Engineering Applications

A Facile Two Step Heat Treatment Strategy for Development of Bioceramic Scaffolds for Hard Tissue Engineering Applications Publisher Pubmed

Farzin A^{1, 2, 3, 4} ; Hassan S^{3, 4} ; Ebrahimibarough S² ; Ai A⁵ ; Hasanzadeh E^{2, 6} ; Goodarzi A² ; Ai J²

Show Affiliations

1. Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Tissue Engineering and Applied Cell Science, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, 02139, MA, United States
4. Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 02139, MA, United States
5. School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
6. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Source: Materials Science and Engineering C Published:2019

Abstract

In the present study, a two-step sintering (TSS) method has been used to improve the mechanical properties, biocompatibility, drug release, and osteogenesis abilities of hardystonite (HT) ceramic scaffolds for tissue engineering and drug delivery applications. The average particle size of HT scaffold is kept lower than 80 nm and is reached higher than 130 nm by using two-step and conventional sintering methods, respectively. The compressive strengths of the prepared nanocrystalline HT scaffolds were found to be significantly higher than those of the micro-structure HT and currently available hydroxyapatite scaffolds. A comparative analysis of cell viability and live/dead staining of human mesenchymal stem cells (hMSCs) in nano- and micro-structured HT scaffolds and their drug release potentiation was carried out. The results showed that the nano-structured HT scaffolds have higher cell viability, biocompatibility and longer-term doxorubicin (DOX) release potential than the micro-structured ones. The results of quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) analyses showed that the expression of adhesion and differentiation supporting genes were significantly higher in nano-structured HT scaffolds as compared to the micro-structured ones. The results of qRT-PCR also showed that the mRNA expression level of ERK1/2 and P38 MAPK from hMSCs were significantly higher in nano-structured HT scaffolds than the micro-structured ones. These results potentially open new aspects for using nano-structured scaffolds in bone tissue engineering applications. © 2019 Elsevier B.V.

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Mahmoud Azami (2)

Somayeh Ebrahimi Barough (1)

Style	Citing Format
MLA	Farzin A, et al.. "A Facile Two Step Heat Treatment Strategy for Development of Bioceramic Scaffolds for Hard Tissue Engineering Applications." Materials Science and Engineering C, vol. 105, no. , 2019, pp. -.
APA	Farzin A, Hassan S, Ebrahimibarough S, Ai A, Hasanzadeh E, Goodarzi A, Ai J (2019). A Facile Two Step Heat Treatment Strategy for Development of Bioceramic Scaffolds for Hard Tissue Engineering Applications. Materials Science and Engineering C, 105(), -.
Chicago	Farzin A, Hassan S, Ebrahimibarough S, Ai A, Hasanzadeh E, Goodarzi A, Ai J. "A Facile Two Step Heat Treatment Strategy for Development of Bioceramic Scaffolds for Hard Tissue Engineering Applications." Materials Science and Engineering C 105, no. (2019): -.
Harvard	Farzin A et al. (2019) 'A Facile Two Step Heat Treatment Strategy for Development of Bioceramic Scaffolds for Hard Tissue Engineering Applications', Materials Science and Engineering C, 105(), pp. -.
Vancouver	Farzin A, Hassan S, Ebrahimibarough S, Ai A, Hasanzadeh E, Goodarzi A, et al.. A Facile Two Step Heat Treatment Strategy for Development of Bioceramic Scaffolds for Hard Tissue Engineering Applications. Materials Science and Engineering C. 2019;105():-.
BibTex	@article{ author = {Farzin A and Hassan S and Ebrahimibarough S and Ai A and Hasanzadeh E and Goodarzi A and Ai J}, title = {A Facile Two Step Heat Treatment Strategy for Development of Bioceramic Scaffolds for Hard Tissue Engineering Applications}, journal = {Materials Science and Engineering C}, volume = {105}, number = {}, pages = {-}, year = {2019} }
RIS	TY - JOUR AU - Farzin A AU - Hassan S AU - Ebrahimibarough S AU - Ai A AU - Hasanzadeh E AU - Goodarzi A AU - Ai J TI - A Facile Two Step Heat Treatment Strategy for Development of Bioceramic Scaffolds for Hard Tissue Engineering Applications JO - Materials Science and Engineering C VL - 105 IS - SP - EP - PY - 2019 ER -

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