Scrophularia Striata Extract Incorporated Nanofibrous Scaffold Improved Osteogenic Differentiation of Mesenchymal Stem Cells

Scrophularia Striata Extract Incorporated Nanofibrous Scaffold Improved Osteogenic Differentiation of Mesenchymal Stem Cells Publisher

Abazari MF¹ ; Karizi SZ² ; Haghanisamani E³ ; Abazari D⁴ ; Zakidizaji M⁵ ; Enderami SE⁶ ; Young PA⁷ ; Le Gratiet KL⁸ ; Tackallou SH⁹ ; Aghapur N¹⁰ ; Mansouri V¹¹

Show Affiliations

1. Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Biology, Varamin Pishva Branch, Islamic Azad University, Pishva, Varamin, Iran
3. Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Science, Tehran, Iran
4. Department of Cellular & Physiological Sciences & the Brain Research Centre, University of British Columbia, 2350 Health Sciences Mall, Vancouver, V6T-1Z3, B.C., Canada
5. Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
6. Immunogenetics Research Center, Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran
7. Department of Biology, University of Victoria, Victoria, BC, Canada
8. Division of Medical Sciences, University of Victoria, Canada
9. Department of Biology, Faculty of Science, Central Tehran Branch, Islamic Azad University, Tehran, Iran
10. Immunogenetics Research Center, Mazandaran University of Medical Sciences, Sari, Iran
11. Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Journal of Drug Delivery Science and Technology Published:2023

Abstract

One of the dynamic challenges of bone tissue engineering has been designing biocompatible and bioactive scaffolds that are highly similar to bone tissue. Composite scaffolds are a combination of polymers and natural materials, and have a high chance of being used in tissue engineering. The use of plant compounds with antibacterial, healing and restorative properties is one of these options. In this study, a composite nanofibrous scaffold was fabricated by Polycaprolactone (PCL) and Scrophularia striata (S.S) extract using electrospinning method. After morphological examination of the constructed scaffolds by SEM, the osteogenic differentiation potential of the Adipose-derived mesenchymal stem cells (ASCs) was evaluated while cultured on the scaffolds. Biocompatibility and non-toxicity of the fabricated scaffolds were confirmed by protein adsorption, MTT and SEM assays. Evaluation of the Alkaline phosphatase activity and calcium production showed that the amount of these two factors in the ASCs cultured on the fabricated scaffold containing the extract (PCL-S.S) increased significantly compared to the cells cultured on the empty scaffolds (PCL). Furthermore, the results obtained by gene and protein expression assays indicated that the expression level of Runx-2, Col-I, osteonectin and osteocalcin genes, and osteocalcin protein increased significantly in the cultured ASCs on the PCL-S.S nanofibers compared to the ASCs cultured on the PCL nanofibers. Taken together, it can be concluded that the osteoinductive property of the PCL scaffolds improved significantly after combining with the S.S extract (PCL-S.S), and this composited-scaffold can be suggested as a promising candidate for use in bone tissue engineering. © 2023 Elsevier B.V.

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Style	Citing Format
MLA	Abazari MF, et al.. "Scrophularia Striata Extract Incorporated Nanofibrous Scaffold Improved Osteogenic Differentiation of Mesenchymal Stem Cells." Journal of Drug Delivery Science and Technology, vol. 89, no. , 2023, pp. -.
APA	Abazari MF, Karizi SZ, Haghanisamani E, Abazari D, Zakidizaji M, Enderami SE, Young PA, Le Gratiet KL, Tackallou SH, Aghapur N, Mansouri V (2023). Scrophularia Striata Extract Incorporated Nanofibrous Scaffold Improved Osteogenic Differentiation of Mesenchymal Stem Cells. Journal of Drug Delivery Science and Technology, 89(), -.
Chicago	Abazari MF, Karizi SZ, Haghanisamani E, Abazari D, Zakidizaji M, Enderami SE, Young PA, et al.. "Scrophularia Striata Extract Incorporated Nanofibrous Scaffold Improved Osteogenic Differentiation of Mesenchymal Stem Cells." Journal of Drug Delivery Science and Technology 89, no. (2023): -.
Harvard	Abazari MF et al. (2023) 'Scrophularia Striata Extract Incorporated Nanofibrous Scaffold Improved Osteogenic Differentiation of Mesenchymal Stem Cells', Journal of Drug Delivery Science and Technology, 89(), pp. -.
Vancouver	Abazari MF, Karizi SZ, Haghanisamani E, Abazari D, Zakidizaji M, Enderami SE, et al.. Scrophularia Striata Extract Incorporated Nanofibrous Scaffold Improved Osteogenic Differentiation of Mesenchymal Stem Cells. Journal of Drug Delivery Science and Technology. 2023;89():-.
BibTex	@article{ author = {Abazari MF and Karizi SZ and Haghanisamani E and Abazari D and Zakidizaji M and Enderami SE and Young PA and Le Gratiet KL and Tackallou SH and Aghapur N and Mansouri V}, title = {Scrophularia Striata Extract Incorporated Nanofibrous Scaffold Improved Osteogenic Differentiation of Mesenchymal Stem Cells}, journal = {Journal of Drug Delivery Science and Technology}, volume = {89}, number = {}, pages = {-}, year = {2023} }
RIS	TY - JOUR AU - Abazari MF AU - Karizi SZ AU - Haghanisamani E AU - Abazari D AU - Zakidizaji M AU - Enderami SE AU - Young PA AU - Le Gratiet KL AU - Tackallou SH AU - Aghapur N AU - Mansouri V TI - Scrophularia Striata Extract Incorporated Nanofibrous Scaffold Improved Osteogenic Differentiation of Mesenchymal Stem Cells JO - Journal of Drug Delivery Science and Technology VL - 89 IS - SP - EP - PY - 2023 ER -

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