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Diversity of Aminoglycoside Modifying Enzymes and 16S Rrna Methylases in Acinetobacter Baumannii and Acinetobacter Nosocomialis Species in Iran; Wide Distribution of Aada1 and Arma Publisher Pubmed



Salimizand H1, 2 ; Zomorodi AR3 ; Mansury D4, 5, 6 ; Khakshoor M7 ; Azizi O8 ; Khodaparast S9, 10 ; Baseri Z10 ; Karami P11, 12 ; Zamanlou S13 ; Farsiani H5, 6 ; Amini Y14, 15 ; Moradi B16 ; Meshkat Z5, 6 ; Salimizand H1, 2 Show All Authors
Authors
  1. Salimizand H1, 2
  2. Zomorodi AR3
  3. Mansury D4, 5, 6
  4. Khakshoor M7
  5. Azizi O8
  6. Khodaparast S9, 10
  7. Baseri Z10
  8. Karami P11, 12
  9. Zamanlou S13
  10. Farsiani H5, 6
  11. Amini Y14, 15
  12. Moradi B16
  13. Meshkat Z5, 6
  14. Salimizand H1, 2
  15. Hasanzadeh S4, 5, 6
  16. Sadeghian H5, 18
Show Affiliations
Authors Affiliations
  1. 1. Liver and Digestive Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
  2. 2. Department of Microbiology, Faculty of Medicine, Kurdistan University of medical Sciences, Sanandaj, Iran
  3. 3. Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi university of Mashhad, Mashhad, Iran
  4. 4. Student Research Committee, Faculty of Medicine, Mashhad, University of Medical Sciences, Mashhad, Iran
  5. 5. Antimicrobial Resistance Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
  6. 6. Department of Microbiology and Virology, Medical School, Mashhad University of medical Sciences, Mashhad, Iran
  7. 7. Microbiology Department, Faculty of science, Islamic Azad University of Tonekabon, Iran
  8. 8. Department of Laboratory Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
  9. 9. Department of bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  10. 10. Molecular laboratory, Shariati hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  11. 11. Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
  12. 12. Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
  13. 13. Department of Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  14. 14. Department of Microbiology, Medical school, Zahedan University of medical Sciences, Zahedan, Iran
  15. 15. Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran
  16. 16. Esfarayen University of Medical Sciences, Esfarayen, Iran
  17. 17. Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
  18. 18. Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran

Source: Infection# Genetics and Evolution Published:2018


Abstract

Purpose: Acinetobacter baumannii-calcoaceticus complex (ABC) make a great burden on health-care systems due to hospital-acquired infections and antibacterial resistance. Aminoglycoside in combination with other antibacterials used as treatment options. However, ABC species overcome this class of antibacterials in different ways. This study provides a comprehensive report on the distribution of aminoglycoside modifying enzymes (AMEs) and 16S rRNA methylase in Acinetobacter baumannii and Acinetobacter nosocomialis isolated from various provinces in Iran. Methods: During six month of study, from eight referral centers in seven provinces across the country, Iran, 178 A. baumannii and 43 A. nosocomialis isolates were collected. The minimum inhibitory concentration of amikacin, gentamicin, netilmicin, kanamycin and tobramycin were measured by microbroth dilution method. AMEs and 16S rRNA methylase variants were sought by PCR. Results: High rates of resistance were seen in all centers. MIC50 and MIC90 for all A. baumannii and A. nosocomialis isolates from different centers were > 512 mg/L. The most frequent AME was ant(3″)-Ia (aadA1) in both of A. baumannii (74.1%) and A. nosocomialis (86%). armA was detected in A. baumannii and A. nosocomialis at the frequency of 41.6% and 67.4%, respectively. rmtA, B, C, D, aac(3)-Ia (aacC1) and aac(6′)-Im were not detected, neither in A. baumannii nor A. nosocomialis. Moreover, aac(6′)-Ih was only found in A. baumannii isolates. The distribution of some of the ARGs was limited to a definite center. Conclusion: The overall high-level carriage of ARGs in Acinetobacter species may limited usage of this class of antibacterials as a treatment option. © 2018 Elsevier B.V.
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