Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Effect of Cryopreservation on Dna Methylation Patterns of the Chromosome 15Q11–Q13 Region in Human Spermatozoa Publisher Pubmed



Khosravizadeh Z1 ; Hassanzadeh G1 ; Tavakkoly Bazzaz J2 ; Alizadeh F3 ; Totonchi M4 ; Salehi E5, 6 ; Khodamoradi K1 ; Khanehzad M1 ; Hosseini SR7 ; Abolhassani F1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Faculty of Anatomy, School of Medicine, Tehran University of Medical Science, 16 Azar Avenue, Poor Sina Street, Tehran, Iran
  2. 2. Faculty of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, 16 Azar Avenue, Poor Sina Street, Tehran, Iran
  3. 3. Faculty of Genomic Psychiatry and Behavioral Genomics (DGPBG), Roozbeh Hospital, School of Medicine, Tehran University of Medical Science, South Kargar Avenue, Tehran, Iran
  4. 4. Faculty of Stem Cells and Developmental Biology, Royan Institute, Tehran, Iran
  5. 5. Faculty of Gynecology, School of Medicine, Fertility and Infertility Research Center, Bandar Abbas, Iran
  6. 6. Dr. Ali Shariati Hospital, Shahid Mohammadi Hospital, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
  7. 7. Faculty of Urology, Sina Hospital, Tehran University of Medical Science, Imam Khomeini Street, Tehran, Iran

Source: Cell and Tissue Banking Published:2020


Abstract

Human sperm cryopreservation is a common technique which is used in assisted reproductive technologies. Despite the existence of evidence supporting the production of ROS and DNA fragmentation during sperm cryopreservation, there is little and equivocal information about the cryopreservation effects on methylation of imprinted genes and imprinting control regions. In this study, we have investigated the effects of cryopreservation on DNA methylation in promoter regions of SNURF-SNRPN and UBE3A imprinted genes, PWS-ICR and AS-ICR in the chromosome 15q11–q13 region. Semen samples from 10 healthy normozoospermic men were collected and each sample was divided into four equal aliquots: fresh, cryoprotectant, cryopreservation, and H2O2. We measured the ROS levels and DNA fragmentation using DCFH-DA and TUNEL assay respectively by flow cytometry. DNA methylation in promoter regions of SNURF-SNRPN and UBE3A imprinted genes, PWS-ICR and AS-ICR in the chromosome 15q11–q13 region were evaluated by quantitative methylation-specific PCR technique. Intracellular levels of ROS and percentage of TUNEL-positive spermatozoa significantly increased in cryopreservation group compared to fresh group. Exposure to cryoprotectant had no significant effect on ROS levels and DNA fragmentation. Neither cryopreservation nor exposure to cryoprotectant significantly affected DNA methylation of the selected gene regions. However, DNA fragmentation had positive correlation with DNA methylation of AS-ICR. In conclusion, based on our study, clinical use of sperm cryopreservation for fertility treatments appear to be safe in regard to DNA methylation in the chromosome 15q11–q13 region. © 2020, Springer Nature B.V.
Related Docs
View other Related Docs
1. Cryoprotective Effect of Resveratrol on Dna Damage and Crucial Human Sperm Messenger Rnas, Possibly Through 5′ Amp-Activated Protein Kinase Activation, Cell and Tissue Banking (2018)
2. Effect of Sulforaphane on Apoptosis, Reactive Oxygen Species and Lipids Peroxidation of Human Sperm During Cryopreservation, Cryobiology (2021)
3. The Role of Antioxidants in Sperm Freezing: A Review, Cell and Tissue Banking (2016)
4. Epigenetic Control of Atherosclerosis Via Dna Methylation: A New Therapeutic Target?, Life Sciences (2020)
5. Protective Effects of Zinc on Rat Sperm Chromatin Integrity Involvement: Dna Methylation, Dna Fragmentation, Ubiquitination and Protamination After Bleomycin Etoposide and Cis-Platin Treatment, Theriogenology (2020)
6. Effect of Trolox on Sperm Quality in Normozospermia and Oligozospermia During Cryopreservation, Cryobiology (2016)
7. Genetic Aspects of Idiopathic Asthenozoospermia As a Cause of Male Infertility, Human Fertility (2020)
8. Resveratrol Alleviates Non-Alcoholic Fatty Liver Disease Through Epigenetic Modification of the Nrf2 Signaling Pathway, International Journal of Biochemistry and Cell Biology (2020)
Experts (# of related papers)
View all Related Experts
Fardin Amidi (7)
Maryam Shabani Nashtaee (6)
Other Related Docs
9. Cryopreservation and Its Effects on Motility and Gene Expression Patterns and Fertilizing Potential of Bovine Epididymal Sperm, Veterinary Medicine and Science (2021)
10. Protective Effects of Hesperetin on the Quality of Sperm, Apoptosis, Lipid Peroxidation, and Oxidative Stress During the Process of Cryopreservation: An Experimental Study, International Journal of Reproductive BioMedicine (2021)
11. Sperm Epigenetics and Male Infertility: Unraveling the Molecular Puzzle, Human Genomics (2024)
12. Does Myoinositol Supplement Improve Sperm Parameters and Dna Integrity in Patients With Oligoasthenoteratozoospermia After the Freezing–Thawing Process?, Cell and Tissue Banking (2020)
13. Aqueous Origanum Vulgare Extract Improves the Quality of Cryopreserved Human Spermatozoa Through Its Antioxidant Effects, Biopreservation and Biobanking (2020)
14. Nerve Growth Factor in Human Semen: Effect of Nerve Growth Factor on the Normozoospermic Men During Cryopreservation Process, Iranian Journal of Basic Medical Sciences (2015)
15. Controversy and Consensus on Indications for Sperm Dna Fragmentation Testing in Male Infertility: A Global Survey, Current Guidelines, and Expert Recommendations, World Journal of Men's Health (2023)
16. Supplementation of Freezing and Thawing Media With Brain-Derived Neurotrophic Factor Protects Human Sperm From Freeze-Thaw-Induced Damage, Fertility and Sterility (2016)
17. Effect of Nerve Growth Factor on Sperm Quality in Asthenozoosprmic Men During Cryopreservation, Reproductive Biology and Endocrinology (2016)
18. Melatonin Affects Membrane Integrity, Intracellular Reactive Oxygen Species, Caspase3 Activity and Akt Phosphorylation in Frozen Thawed Human Sperm, Cell and Tissue Research (2018)
19. The Effect of the Ovarian Varicose Vein on the Dna Methylation in the Rat’S Oocyte, Iranian Journal of Basic Medical Sciences (2017)
20. Leptin and Prolactin Reduce Cryodamage in Normozoospermic Human Semen Samples During Cryopreservation; [La Leptina Y La Prolactina Reducen El Criodano En Muestras De Semen Humano Normozoospermico Durante La Criopreservacion], Revista Internacional de Andrologia (2023)